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[185489_at]

Description R12C12.4 /REP_DB=WormBase Gene ID /WP=CE02849 /TR=Q21958 /GB=AAC46783.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 55      4grp_total > 36      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 50
1 2 3 4 range
A 53.5
(3)
A 		44.6
(0.6)
A 		31.7
(-3)
A 		39
(-1)
A 		22
B 22
(-3)
A 		49
(3)
A 		48
(2.8)
A 		30.8
(-1)
A 		27
C 27.5
(3)
A 		10.3
(-1.2)
A 		3.1
(-3)
A 		25.9
(2.6)
A 		24
avr 34.3
(2.7) 		34.6
(3) 		27.6
(-3) 		31.9
(0.7) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 43
1 2 3 4 5 6 range
A 31.9
(1.3)
A 		31.7
(1.2)
A 		42.4
(3)
A 		7.8
(-2.7)
A 		5.8
(-3)
A 		21.7
(-0.4)
A 		37
B 27.8
(0)
A 		12.7
(-2.3)
A 		39.5
(1.8)
A 		15.7
(-1.8)
A 		8
(-3)
A 		47
(3)
A 		39
C 4.3
(-3)
A 		16.8
(1)
A 		12.7
(-0.3)
A 		23.1
(3)
A 		14.3
(0.2)
A 		13.2
(-0.2)
A 		19
avr 21.3
(0.2) 		20.4
(-0) 		31.5
(3) 		15.5
(-1.3) 		9.4
(-3) 		27.3
(1.9) 		22
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 19.5
(3)
A 		9.7
(-1.3)
A 		8.3
(-1.9)
A 		5.7
(-3)
A 		14
B 7
(-2.6)
A 		12.1
(3)
A 		11.1
(1.9)
A 		6.6
(-3)
A 		6
C 9.1
(3)
A 		3
(-1.6)
A 		1.1
(-3)
A 		4.4
(-0.5)
A 		8
avr 11.9
(3) 		8.3
(-0.4) 		6.8
(-1.9) 		5.6
(-3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 5.9
(-0.4)
A 		11.3
(3)
A 		9.2
(1.7)
A 		2
(-2.9)
A 		1.8
(-3)
A 		5.3
(-0.8)
A 		10
B 9.7
(-0.3)
A 		4.2
(-2.6)
A 		9.6
(-0.3)
A 		4.2
(-2.6)
A 		3.3
(-3)
A 		17.5
(3)
A 		14
C 1.6
(-3)
A 		5.1
(3)
A 		3.2
(-0.3)
A 		4.6
(2.1)
A 		4.6
(2.1)
A 		4.4
(1.8)
A 		4
avr 5.7
(-0.5) 		6.9
(0.8) 		7.3
(1.2) 		3.6
(-2.6) 		3.2
(-3) 		9.1
(3) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185489_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4908
Sequence Type Exemplar sequence
Representative Public ID R12C12.4 NCBI
Target Description R12C12.4 /REP_DB=WormBase Gene ID /WP=CE02849 /TR=Q21958 /GB=AAC46783.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062804(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062804 NCBI Caenorhabditis elegans R12C12.4 (R12C12.4) mRNA, complete cds. 11/11 None
R12C12.4 ENSEMBL cdna:known chromosome:CEL140:II:6039300:6039606:-1 gene:R12C12.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6039296-6039603(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R12C12.4
Entrez Gene 187839 Entrez gene
SwissProt Q21958 EMBL-EBI
WormBase CE02849 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_495205 R12C12.4 [Caenorhabditis elegans] gb|AAC46783.1| Hypothetical protein R12C12.4 [Caenorhabditis elegans] 1.0E-36
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495205.1 1 26-48
R12C12.4 1 26-48
Sequence
Target Sequence 
>C. ELEGANS:185489_AT
atgagaaccttcccaaaaatgacacgattaagaaccaaagcagcccatcgagttggcgtc
atgaagcttaagcgttttcttattttcttctctcctccgtttgttttccatcttcttcca
atggtagtggagtgcgggggttggtcactgcactcccaccacaatgtttattttcgcacc
gaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGAGAACCTTCCCAAAAATGACAC 368 41 13 Antisense
GACACGATTAAGAACCAAAGCAGCC 324 367 33 Antisense
CAAAGCAGCCCATCGAGTTGGCGTC 624 191 48 Antisense
GCCCATCGAGTTGGCGTCATGAAGC 241 287 55 Antisense
GTTGGCGTCATGAAGCTTAAGCGTT 12 437 64 Antisense
ATGAAGCTTAAGCGTTTTCTTATTT 17 45 73 Antisense
TTTTCCATCTTCTTCCAATGGTAGT 697 677 116 Antisense
ATGGTAGTGGAGTGCGGGGGTTGGT 206 23 133 Antisense
GAGTGCGGGGGTTGGTCACTGCACT 22 395 142 Antisense
GCACTCCCACCACAATGTTTATTTT 206 315 162 Antisense
CCACAATGTTTATTTTCGCACCGAA 684 275 171 Antisense
Sequence Source Affymetrix Proprietary Database