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[185480_s_at]

Description F36H12.8 /REP_DB=WormBase Gene ID /WP=CE09979 /TR=O76712 /GB=AAC26932.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 98      4grp_total > 10      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 6.1
(-3)
A 		15.2
(-2)
A 		8.6
(-2.7)
A 		59.3
(3)
A 		53
B 35.7
(1)
A 		51.1
(3)
A 		4.9
(-3)
A 		38.7
(1.4)
A 		46
C 16.2
(-1.8)
A 		4.5
(-3)
A 		39.7
(0.5)
A 		64.6
(3)
A 		60
avr 19.3
(-2.7) 		23.6
(-2) 		17.7
(-3) 		54.2
(3) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 350
1 2 3 4 5 6 range
A 38
(-2.5)
A 		7.3
(-3)
A 		29.5
(-2.6)
A 		54
(-2.2)
A 		232.1
(0.9)
P 		354.6
(3)
P 		347
B 28.8
(-2.9)
A 		34.9
(-2.8)
A 		24.2
(-3)
A 		29.1
(-2.9)
A 		231.1
(1.6)
P 		296.2
(3)
P 		272
C 24.2
(-2.7)
A 		26.7
(-2.6)
A 		4.6
(-3)
A 		41.6
(-2.4)
A 		208.6
(0.6)
P 		349.1
(3)
P 		345
avr 30.3
(-2.8) 		23
(-2.9) 		19.4
(-3) 		41.6
(-2.6) 		223.9
(0.9) 		333.3
(3) 		314
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 2.2
(-3)
A 		3.3
(-2)
A 		2.2
(-3)
A 		8.7
(3)
A 		7
B 11.4
(2.4)
A 		12.6
(3)
A 		1.1
(-3)
A 		8.2
(0.7)
A 		12
C 5.3
(-1.1)
A 		1.3
(-3)
A 		13.8
(3)
A 		10.9
(1.6)
A 		13
avr 6.3
(-2) 		5.7
(-3) 		5.7
(-3) 		9.3
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 116
1 2 3 4 5 6 range
A 7
(-2.7)
A 		2.6
(-3)
A 		6.4
(-2.7)
A 		13.7
(-2.2)
A 		70
(1.8)
P 		86.3
(3)
P 		84
B 10
(-2.8)
A 		11.6
(-2.7)
A 		5.9
(-3)
A 		7.7
(-2.9)
A 		93.9
(2)
P 		110.5
(3)
P 		105
C 9
(-2.6)
A 		8.1
(-2.6)
A 		1.2
(-3)
A 		8.4
(-2.6)
A 		67.5
(0.4)
P 		116.8
(3)
P 		116
avr 8.7
(-2.7) 		7.4
(-2.8) 		4.5
(-3) 		9.9
(-2.7) 		77.1
(1.4) 		104.5
(3) 		100
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 185480_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10943
Sequence Type Exemplar sequence
Representative Public ID F36H12.8 NCBI
Target Description F36H12.8 /REP_DB=WormBase Gene ID /WP=CE09979 /TR=O76712 /GB=AAC26932.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068314(11), NM_068358(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068314 NCBI Caenorhabditis elegans R13H9.5 (R13H9.5) mRNA, complete cds. 11/11 None
NM_068358 NCBI Caenorhabditis elegans F36H12.8 (F36H12.8) mRNA, complete cds. 11/11 None
SNAP00000002638 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5258745:5259909:1 11/11 None
GENEFINDER00000002658 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5258745:5259909:1 11/11 None
SNAP00000029406 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5065742:5066906:-1 11/11 None
GENEFINDER00000029411 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5065742:5066906:-1 11/11 None
R13H9.5 ENSEMBL cdna:known chromosome:CEL140:IV:5065736:5066906:-1 gene:R13H9.5 11/11 None
F36H12.8 ENSEMBL cdna:known chromosome:CEL140:IV:5258704:5259961:1 gene:F36H12.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5258750-5259915(+) 100.0 100.0
chrIV:5065747-5066912(-) 99.68 99.68
Public Domain and Genome References
Gene Title

Ensembl R13H9.5
F36H12.8
Entrez Gene 177276 Entrez gene
177304 Entrez gene
SwissProt O76712 EMBL-EBI
Q966G2 EMBL-EBI
WormBase CE09979 Wormbase
CE07449 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK85494 Hypothetical protein R13H9.5 [Caenorhabditis elegans] ref|NP_500715.1| R13H9.5 [Caenorhabditis elegans] 1.0E-175
blast AAC26932 Hypothetical protein F36H12.8 [Caenorhabditis elegans] ref|NP_500759.1| F36H12.8 [Caenorhabditis elegans] 1.0E-175
blast AAC26932 Hypothetical protein F36H12.8 [Caenorhabditis elegans] ref|NP_500759.1| F36H12.8 [Caenorhabditis elegans] 1.0E-174
blast AAK85494 Hypothetical protein R13H9.5 [Caenorhabditis elegans] ref|NP_500715.1| R13H9.5 [Caenorhabditis elegans] 1.0E-174
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.3E-9
Sequence
Target Sequence 
>C. ELEGANS:185480_S_AT
caatgtttgagtctggcctgttctctcagtgtcggaatccaatccttggaagctcttgag
gatctccacaacattggatacctgcaccgtgacgtgaagcccggaaactatacgattggc
cgtgccgagttgaatgaacttcgtaaagtttatatcctcgacttcggaatggctcgcaag
ttcacggacaacaatggagtgatccgcaaaccaagagctgccgccggattccgtggaact
gttcgctatgcgccaatcgcttgccataagaatcaggagcttggaagaaaagatgacgtg
gaggtttggctctacatgcaagttgagctgactgttggacgtgttccatggaaagagatc
accgacatgaacgcagttggacaagccaagcaagcgatccgcaatactccagaaaagatg
tttgtcttcccgtgcccagccaacgagttgaaggaaattatgaagatggttgatagctgg
gactactttgccgatcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAATGTTTGAGTCTGGCCTGTTCTC 298 183 340 Antisense
CTGTTCTCTCAGTGTCGGAATCCAA 367 235 357 Antisense
GGAAGCTCTTGAGGATCTCCACAAC 316 531 387 Antisense
CAACATTGGATACCTGCACCGTGAC 417 189 408 Antisense
GCACCGTGACGTGAAGCCCGGAAAC 625 295 423 Antisense
TCGGAATGGCTCGCAAGTTCACGGA 141 607 503 Antisense
TGATCCGCAAACCAAGAGCTGCCGC 553 565 539 Antisense
GCGCCAATCGCTTGCCATAAGAATC 642 287 589 Antisense
GACGTGGAGGTTTGGCTCTACATGC 534 375 634 Antisense
TTGAGCTGACTGTTGGACGTGTTCC 36 707 662 Antisense
ATAGCTGGGACTACTTTGCCGATCC 482 21 812 Antisense
Sequence Source Affymetrix Proprietary Database