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[185468_at]

Description Y75B8A.20 /REP_DB=WormBase Gene ID /WP=CE23031 /TR=Q9XW83 /GB=CAA22086.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 35      6grp_high1_10_10 > 43      6grp_highest_nonorm > 33      6grp_total > 20      4grp_total > 49      6grp_highest_sub > 03      6grp_cgc > 73      6grp_high1_sub > 02      6grp_cgc_sub > 02_03      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4765
1 2 3 4 range
A 93
(-3)
A 		4155.9
(2.9)
P 		4228.7
(3)
P 		166
(-2.9)
P 		4136
B 78.8
(-3)
A 		4364.6
(2.4)
P 		4843.6
(3)
P 		144.1
(-2.9)
P 		4765
C 100.9
(-3)
A 		3949.3
(2.9)
P 		3982.8
(3)
P 		142.9
(-2.9)
P 		3882
avr 90.9
(-3) 		4156.6
(2.7) 		4351.7
(3) 		151
(-2.9) 		4261

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2792
1 2 3 4 5 6 range
A 263.8
(-3)
P 		2838.6
(3)
P 		2524.3
(2.3)
P 		1831.4
(0.7)
P 		1295.5
(-0.6)
P 		565.2
(-2.3)
P 		2575
B 226.9
(-3)
P 		2929.7
(3)
P 		2494.3
(2)
P 		1864.8
(0.6)
P 		1312.2
(-0.6)
P 		562.2
(-2.3)
P 		2703
C 146
(-3)
P 		2937.6
(3)
P 		2026.4
(1)
P 		1842
(0.6)
P 		1208.2
(-0.7)
P 		612.7
(-2)
P 		2792
avr 212.2
(-3) 		2902
(3) 		2348.3
(1.8) 		1846.1
(0.6) 		1272
(-0.6) 		580
(-2.2) 		2690
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1364
1 2 3 4 range
A 33.8
(-2.9)
A 		902.1
(1.9)
P 		1105.5
(3)
P 		24.3
(-3)
P 		1081
B 25.1
(-3)
A 		1078
(2.8)
P 		1122.4
(3)
P 		30.7
(-3)
P 		1097
C 33.4
(-3)
A 		1135.5
(1.9)
P 		1388.1
(3)
P 		24.1
(-3)
P 		1364
avr 30.8
(-3) 		1038.5
(2.2) 		1205.3
(3) 		26.4
(-3) 		1179

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 961
1 2 3 4 5 6 range
A 48.6
(-3)
P 		1009.5
(3)
P 		549.7
(0.1)
P 		464.5
(-0.4)
P 		390.9
(-0.9)
P 		137.6
(-2.4)
P 		961
B 79
(-3)
P 		975.2
(3)
P 		605.7
(0.5)
P 		493.9
(-0.2)
P 		533.1
(0)
P 		209.6
(-2.1)
P 		896
C 54.1
(-3)
P 		896.3
(3)
P 		515.8
(0.3)
P 		370.4
(-0.7)
P 		390.9
(-0.6)
P 		205.1
(-1.9)
P 		842
avr 60.6
(-3) 		960.3
(3) 		557.1
(0.3) 		442.9
(-0.5) 		438.3
(-0.5) 		184.1
(-2.2) 		900
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185468_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6265
Sequence Type Exemplar sequence
Representative Public ID Y75B8A.20 NCBI
Target Description Y75B8A.20 /REP_DB=WormBase Gene ID /WP=CE23031 /TR=Q9XW83 /GB=CAA22086.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067191(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067191 NCBI Caenorhabditis elegans GRound-Like (grd related) family member (grl-15) (grl-15) mRNA, complete cds. 11/11 None
SNAP00000020083 ENSEMBL cdna:SNAP chromosome:CEL140:III:12224508:12235524:-1 11/11 None
GENEFINDER00000020186 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:12232604:12235524:-1 11/11 None
Y75B8A.20 ENSEMBL cdna:known chromosome:CEL140:III:12232526:12235576:-1 gene:Y75B8A.20 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:12232599-12235520(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol grl-15
Ensembl Y75B8A.20
Entrez Gene 190707 Entrez gene
SwissProt Q9XW83 EMBL-EBI
WormBase CE23031 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA22084 Hypothetical protein Y75B8A.17 [Caenorhabditis elegans] ref|NP_499589.1| GeMiNin homolog family member (gmn-1) [Caenorhabditis elegans] dbj|BAD98298.1| geminin homolog [Caenorhabditis elegans] 4.0E-88
blast CAA22086 Hypothetical protein Y75B8A.20 [Caenorhabditis elegans] ref|NP_499592.1| GRound-Like (grd related) family member (grl-15) [Caenorhabditis elegans] 3.0E-63
blast CAA22086 Hypothetical protein Y75B8A.20 [Caenorhabditis elegans] ref|NP_499592.1| GRound-Like (grd related) family member (grl-15) [Caenorhabditis elegans] 6.0E-62
blast CAE56552 Hypothetical protein CBG24285 [Caenorhabditis briggsae] 2.0E-48
blast CAE56552 Hypothetical protein CBG24285 [Caenorhabditis briggsae] 3.0E-47
blast CAE58345 Hypothetical protein CBG01466 [Caenorhabditis briggsae] 6.0E-11
Protein Domains
Database ID Description E-Value
Pfam IPR007284 EMBL-EBI Protein of unknown function DUF398 6.6E-27
Sequence
Target Sequence 
>C. ELEGANS:185468_AT
aacggtttcgtgcacatttttcaatggaggatgtggatgtcaacctccaccaatttgcct
tccacctccaccaccaatgtgtttcactgaaattcaattgccgccaattcggattccaat
aatcccgttgccacgtatcgaacttccacagccgtgttgcccaacttgtgcgtgcggtgg
aagaaagaagagagaaatcgacgcaggtgtccaagaggaagaatcagttagtaccaagga
tgtctcttgcaatgatgactctcttttggcaatcatgaaaaaggaaatgacaaccggaga
gtcttcagctgtcaagattgcactagttgaggctgccgagcaagagctgggcggtagatt
cactgtagtttgtagtcaaggtgcattctcatttgtcacttctacaacaagttactgcct
tcactca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACGGTTTCGTGCACATTTTTCAAT 106 143 57 Antisense
GATGTGGATGTCAACCTCCACCAAT 136 409 86 Antisense
AATTGCCGCCAATTCGGATTCCAAT 306 177 152 Antisense
CAATAATCCCGTTGCCACGTATCGA 227 179 173 Antisense
CACGTATCGAACTTCCACAGCCGTG 521 195 188 Antisense
GCAATGATGACTCTCTTTTGGCAAT 109 323 305 Antisense
ACAACCGGAGAGTCTTCAGCTGTCA 318 113 346 Antisense
TTGCACTAGTTGAGGCTGCCGAGCA 521 703 374 Antisense
GAGCTGGGCGGTAGATTCACTGTAG 285 387 400 Antisense
CAAGGTGCATTCTCATTTGTCACTT 109 185 433 Antisense
TACAACAAGTTACTGCCTTCACTCA 226 643 459 Antisense
Sequence Source Affymetrix Proprietary Database