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[185467_at]

Description Y23H5A.6 /REP_DB=WormBase Gene ID /WP=CE24191 /TR=O76613 /GB=AAC64632.2 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 10      6grp_high1_10_10 > 07      4grp_highest_8_8 > 23      4grp_high_8_8 > 13      6grp_highest_nonorm > 50      6grp_total > 04      4grp_total > 03      6grp_cgc > 40      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1546
1 2 3 4 range
A 539.2
(-2.6)
P 		432.8
(-3)
P 		445.7
(-2.9)
P 		1921.8
(3)
P 		1489
B 593.1
(-2.1)
P 		426.2
(-2.8)
P 		375.5
(-3)
P 		1756.3
(3)
P 		1381
C 561
(-2.2)
P 		569.2
(-2.2)
P 		428.2
(-3)
P 		1452.5
(3)
P 		1024
avr 564.4
(-2.3) 		476.1
(-2.7) 		416.5
(-3) 		1710.2
(3) 		1294

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 499
1 2 3 4 5 6 range
A 716.2
(3)
P 		532.3
(0)
P 		343.3
(-3)
P 		441.7
(-1.4)
P 		461.4
(-1.1)
P 		371
(-2.6)
P 		373
B 689.6
(3)
P 		624.8
(1.9)
P 		460.8
(-0.7)
P 		552.3
(0.8)
P 		362.3
(-2.3)
P 		320.8
(-3)
P 		369
C 736.3
(3)
P 		580.2
(1.1)
P 		466.5
(-0.2)
P 		606.5
(1.4)
P 		339
(-1.8)
P 		237.5
(-3)
P 		499
avr 714
(3) 		579.1
(1) 		423.5
(-1.3) 		533.5
(0.3) 		387.6
(-1.8) 		309.8
(-3) 		404
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 287
1 2 3 4 range
A 196.3
(0.3)
P 		94
(-3)
P 		116.5
(-2.3)
P 		281.3
(3)
P 		187
B 189.1
(-0.9)
P 		105.3
(-2.6)
P 		87
(-3)
P 		374.3
(3)
P 		287
C 185.7
(-0.7)
P 		163.6
(-2.1)
P 		149.2
(-3)
P 		244.9
(3)
P 		96
avr 190.4
(-0.6) 		121
(-2.9) 		117.6
(-3) 		300.2
(3) 		183

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 198
1 2 3 4 5 6 range
A 132
(-0)
P 		189.3
(3)
P 		74.8
(-3)
P 		112
(-1.1)
P 		139.2
(0.4)
P 		90.3
(-2.2)
P 		115
B 239.9
(3)
P 		208
(1.5)
P 		111.9
(-3)
P 		146.3
(-1.4)
P 		147.2
(-1.3)
P 		119.6
(-2.6)
P 		128
C 272.9
(3)
P 		177
(0)
P 		118.7
(-1.8)
P 		122
(-1.7)
P 		109.7
(-2.1)
P 		79.5
(-3)
P 		193
avr 214.9
(3) 		191.4
(1.8) 		101.8
(-2.7) 		126.8
(-1.5) 		132
(-1.2) 		96.5
(-3) 		118
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185467_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.308
Sequence Type Exemplar sequence
Representative Public ID Y23H5A.6 NCBI
Target Description Y23H5A.6 /REP_DB=WormBase Gene ID /WP=CE24191 /TR=O76613 /GB=AAC64632.2 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_170830(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_170830 NCBI Caenorhabditis elegans alpha-CaTuliN (catenin/vinculin related) family member (ctn-1) (ctn-1) mRNA, complete cds. 11/11 None
SNAP00000016933 ENSEMBL cdna:SNAP chromosome:CEL140:I:2606755:2613151:1 11/11 None
GENEFINDER00000016951 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:2606784:2613151:1 11/11 None
Y23H5A.5c ENSEMBL cdna:known chromosome:CEL140:I:2606835:2613275:1 gene:Y23H5A.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_170829 7/11 Cross Hyb Matching Probes A B 
NM_058607 7/11 Cross Hyb Matching Probes A B 
NM_058608 7/11 Cross Hyb Matching Probes A B 
Y23H5A.5d.2 7/11 Cross Hyb Matching Probes A B 
Y23H5A.5d.1 7/11 Cross Hyb Matching Probes A B 
Y23H5A.5b 7/11 Cross Hyb Matching Probes A B 
Y23H5A.5a 7/11 Cross Hyb Matching Probes A B 
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
171818 4 1 NM_058607 187858_at (11)
NM_058608 187858_at (10)
NM_170829 187858_at (11)
NM_170830 185467_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:2606834-2613151(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y23H5A.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_740814 alpha-CaTuliN (catenin/vinculin related) family member (ctn-1) [Caenorhabditis elegans] gb|AAM19065.1| Alpha-catulin (catenin/vinculin related) protein 1, isoform c [Caenorhabditis elegans] 0.0
blast NP_740813 alpha-CaTuliN (catenin/vinculin related) family member (ctn-1) [Caenorhabditis elegans] gb|AAM54172.1| Alpha-catulin (catenin/vinculin related) protein 1, isoform d [Caenorhabditis elegans] 0.0
blast NP_491009 alpha-CaTuliN (catenin/vinculin related) family member (ctn-1) [Caenorhabditis elegans] gb|AAL77190.1| Alpha-catulin (catenin/vinculin related) protein 1, isoform b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006077 EMBL-EBI Vinculin/alpha-catenin 8.4E-40
Pfam IPR006077 EMBL-EBI Vinculin/alpha-catenin 8.4E-40
Pfam IPR006077 EMBL-EBI Vinculin/alpha-catenin 8.4E-40
Sequence
Target Sequence 
>C. ELEGANS:185467_AT
agttccgggagcacgcggatcacatgcaagaggtatgccgcctcctgcatcatatctcaa
tcacagacgccctacacgttcacaccgggcatgtcgagcggaatatgagagccctcgccc
cattgacaattctcgctggccggacgctttgtgtgcacccatcttcgaggatagccaggg
agaacttggaggtgttctgtgacacctgggggcaggcggtgaatgatctctcgcgtgtcg
ccaaagaatccgatgtcgccgcaaatggaagagttgctgctgaaaagcaggcgtacatgt
cgctcccgcgaccaggggtaagtaaatcatcttgccgtcgatctgtacccaagcatgacg
ctgcttcttcttcttcatctaacgagcttttgccaaacggcggtcgacaccccaaacagg
ttggaatagcgccaactcgagtcgtcagtcagacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGTTCCGGGAGCACGCGGATCACAT 112 65 1310 Antisense
ATCATATCTCAATCACAGACGCCCT 39 31 1358 Antisense
TTCACACCGGGCATGTCGAGCGGAA 667 685 1388 Antisense
GAGCGGAATATGAGAGCCCTCGCCC 211 387 1405 Antisense
GGCGGTGAATGATCTCTCGCGTGTC 158 541 1524 Antisense
GCCAAAGAATCCGATGTCGCCGCAA 537 277 1549 Antisense
AAAAGCAGGCGTACATGTCGCTCCC 178 133 1592 Antisense
GTAAATCATCTTGCCGTCGATCTGT 54 459 1631 Antisense
GTCGATCTGTACCCAAGCATGACGC 420 473 1646 Antisense
GCTTTTGCCAAACGGCGGTCGACAC 624 301 1695 Antisense
GCCAACTCGAGTCGTCAGTCAGACC 707 275 1740 Antisense
Sequence Source Affymetrix Proprietary Database