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[185453_at]

Description H14A12.5 /REP_DB=WormBase Gene ID /WP=CE11586 /TR=O17215 /GB=AAB70981.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 86      4grp_total > 31      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 89
1 2 3 4 range
A 127.8
(3)
A 		69.7
(-1.9)
A 		56.6
(-3)
A 		86.1
(-0.5)
A 		71
B 98
(3)
A 		43.9
(-3)
A 		68.1
(-0.3)
A 		86.4
(1.7)
A 		54
C 122.9
(3)
A 		38.9
(-3)
A 		45.2
(-2.6)
A 		83.5
(0.2)
A 		84
avr 116.2
(3) 		50.8
(-3) 		56.6
(-2.5) 		85.3
(0.2) 		65

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 149
1 2 3 4 5 6 range
A 68.2
(-3)
A 		104.2
(-1.6)
A 		217.2
(3)
P 		107
(-1.4)
A 		177.2
(1.4)
P 		180.5
(1.5)
P 		149
B 90.9
(-3)
A 		143.4
(0.1)
A 		151.5
(0.6)
P 		102.2
(-2.3)
A 		192.1
(3)
P 		175.5
(2)
P 		101
C 70.8
(-3)
A 		130.8
(-0.2)
P 		88.6
(-2.2)
A 		199.7
(3)
P 		147.5
(0.6)
P 		177.5
(2)
P 		129
avr 76.6
(-3) 		126.1
(-0.1) 		152.4
(1.5) 		136.3
(0.5) 		172.3
(2.7) 		177.8
(3) 		101
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 36
1 2 3 4 range
A 46.5
(3)
A 		15.1
(-2.6)
A 		14.8
(-2.6)
A 		12.6
(-3)
A 		34
B 31.3
(3)
A 		10.8
(-3)
A 		15.8
(-1.5)
A 		18.4
(-0.8)
A 		21
C 40.7
(3)
A 		11.2
(-3)
A 		15.7
(-2.1)
A 		14.1
(-2.4)
A 		30
avr 39.5
(3) 		12.4
(-3) 		15.4
(-2.3) 		15
(-2.4) 		27

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 66
1 2 3 4 5 6 range
A 12.6
(-3)
A 		37.1
(0.6)
A 		47.3
(2.1)
P 		27.1
(-0.9)
A 		53.5
(3)
P 		44
(1.6)
P 		41
B 31.6
(-2.5)
A 		47.7
(-0.6)
A 		36.8
(-1.9)
P 		27.1
(-3)
A 		78.1
(3)
P 		65.4
(1.5)
P 		51
C 26.2
(-2.4)
A 		39.9
(-0.2)
P 		22.5
(-3)
A 		40.2
(-0.1)
P 		47.7
(1.1)
P 		59.4
(3)
P 		37
avr 23.5
(-3) 		41.6
(-0) 		35.5
(-1) 		31.5
(-1.7) 		59.8
(3) 		56.3
(2.4) 		36
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185453_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6973
Sequence Type Exemplar sequence
Representative Public ID H14A12.5 NCBI
Target Description H14A12.5 /REP_DB=WormBase Gene ID /WP=CE11586 /TR=O17215 /GB=AAB70981.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066242(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066242 NCBI Caenorhabditis elegans H14A12.5 (H14A12.5) mRNA, complete cds. 11/11 None
SNAP00000033736 ENSEMBL cdna:SNAP chromosome:CEL140:III:7467515:7468009:-1 11/11 None
H14A12.5 ENSEMBL cdna:known chromosome:CEL140:III:7467515:7468009:-1 gene:H14A12.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000033741 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7467512-7468007(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl H14A12.5
Entrez Gene 186742 Entrez gene
SwissProt O17215 EMBL-EBI
WormBase CE11586 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB70981 Hypothetical protein H14A12.5 [Caenorhabditis elegans] ref|NP_498643.1| H14A12.5 [Caenorhabditis elegans] 1.0E-71
blast CAE64463 Hypothetical protein CBG09179 [Caenorhabditis briggsae] 4.0E-55
Sequence
Target Sequence 
>C. ELEGANS:185453_AT
gcatgacgtcatggagctacggaatctatcgatggttgatctgcaaacttttaatacaca
attttccaagataactgatcgatattggagagaaattttcagaaggcaaaatgattccca
tcatcctgatcatattaatgtgtcacggttacaaactgcgaatattattactgttctaga
tgagaatctacctcaaggagctattcaggaactcaccataactggtctaataccaaaacc
attttcctctcgacggtgtgaagtaatgcgatacctgagagagcggctatcgtgttctcc
ggatgtcgaattaagctttcaaggcgatcaacttaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCATGACGTCATGGAGCTACGGAAT 517 293 15 Antisense
GGAATCTATCGATGGTTGATCTGCA 67 533 35 Antisense
TGATTCCCATCATCCTGATCATATT 135 565 126 Antisense
AATGTGTCACGGTTACAAACTGCGA 268 163 151 Antisense
GAGAATCTACCTCAAGGAGCTATTC 393 391 196 Antisense
GGAGCTATTCAGGAACTCACCATAA 521 521 211 Antisense
TAATACCAAAACCATTTTCCTCTCG 91 635 242 Antisense
CATTTTCCTCTCGACGGTGTGAAGT 629 215 254 Antisense
ATGCGATACCTGAGAGAGCGGCTAT 511 41 280 Antisense
CTATCGTGTTCTCCGGATGTCGAAT 669 223 301 Antisense
AAGCTTTCAAGGCGATCAACTTAGA 438 147 327 Antisense
Sequence Source Affymetrix Proprietary Database