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[185451_at]

Description F35D11.3 /REP_DB=WormBase Gene ID /WP=CE02716 /TR=Q20035 /GB=AAA68758.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 90      6grp_high1_10_10 > 09      4grp_highest_8_8 > 30      4grp_high_8_8 > 04      6grp_highest_nonorm > 71      6grp_total > 29      4grp_total > 04      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 809
1 2 3 4 range
A 351.1
(-1.3)
P 		179.2
(-2.9)
P 		172
(-3)
P 		811.7
(3)
P 		640
B 333.6
(-1.5)
P 		139.1
(-3)
P 		155
(-2.9)
P 		910.8
(3)
P 		772
C 346.4
(-1.6)
P 		155.5
(-3)
P 		170.1
(-2.9)
P 		948.1
(3)
P 		793
avr 343.7
(-1.5) 		157.9
(-3) 		165.7
(-2.9) 		890.2
(3) 		732

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 157
1 2 3 4 5 6 range
A 393.6
(3)
P 		325.9
(-1.8)
P 		309.9
(-3)
P 		363.9
(0.9)
P 		388.3
(2.6)
P 		309.7
(-3)
P 		84
B 374.1
(-0.3)
P 		425.4
(3)
P 		332.6
(-3)
P 		361.3
(-1.1)
P 		403.3
(1.6)
P 		347
(-2.1)
P 		93
C 352
(3)
P 		349.7
(2.8)
P 		268.6
(-3)
P 		293.2
(-1.2)
P 		343.5
(2.4)
P 		326.4
(1.2)
P 		83
avr 373.2
(2.6) 		367
(2.1) 		303.7
(-3) 		339.5
(-0.1) 		378.4
(3) 		327.7
(-1.1) 		75
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 160
1 2 3 4 range
A 127.8
(3)
P 		38.9
(-3)
P 		45
(-2.6)
P 		118.8
(2.4)
P 		89
B 106.4
(-0.3)
P 		34.3
(-3)
P 		35.9
(-2.9)
P 		194.1
(3)
P 		160
C 114.7
(0.6)
P 		44.7
(-3)
P 		59.3
(-2.2)
P 		159.9
(3)
P 		115
avr 116.3
(0.9) 		39.3
(-3) 		46.7
(-2.6) 		157.6
(3) 		118

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 105
1 2 3 4 5 6 range
A 72.6
(-2.4)
P 		115.9
(2.8)
P 		67.5
(-3)
P 		92.3
(-0)
P 		117.2
(3)
P 		75.4
(-2)
P 		50
B 130.2
(0.6)
P 		141.6
(1.4)
P 		80.8
(-3)
P 		95.7
(-1.9)
P 		163.9
(3)
P 		129.4
(0.5)
P 		83
C 130.5
(3)
P 		106.7
(1)
P 		68.4
(-2.2)
P 		59
(-3)
P 		111.1
(1.4)
P 		109.3
(1.2)
P 		72
avr 111.1
(1) 		121.4
(2) 		72.2
(-3) 		82.3
(-2) 		130.7
(3) 		104.7
(0.3) 		59
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185451_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4671
Sequence Type Exemplar sequence
Representative Public ID F35D11.3 NCBI
Target Description F35D11.3 /REP_DB=WormBase Gene ID /WP=CE02716 /TR=Q20035 /GB=AAA68758.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062411(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062411 NCBI Caenorhabditis elegans F35D11.3 (F35D11.3) mRNA, complete cds. 11/11 A 
SNAP00000013540 ENSEMBL cdna:SNAP chromosome:CEL140:II:4609086:4611929:1 11/11 None
GENEFINDER00000013553 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4608405:4612309:1 10/11 None
F35D11.3.2 ENSEMBL cdna:known chromosome:CEL140:II:4608357:4612026:1 gene:F35D11.3 11/11 None
F35D11.3.1 ENSEMBL cdna:known chromosome:CEL140:II:4608359:4612026:1 gene:F35D11.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4609007-4611236(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F35D11.3
Entrez Gene 173798 Entrez gene
SwissProt Q20035 EMBL-EBI
WormBase CE31803 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.23276.1.S1_S_AT similar to transmembrane and coiled-coil domains 4 cfa
CANINE_2:CFAAFFX.23281.1.S1_AT similar to transmembrane and coiled-coil domains 4 cfa
CANINE_2:CFAAFFX.23283.1.S1_S_AT similar to transmembrane and coiled-coil domains 4 cfa
CHICKEN:GGAAFFX.2522.1.S1_AT similar to hypothetical protein LOC255104 gga
CHICKEN:GGAAFFX.2522.2.S1_S_AT similar to hypothetical protein LOC255104 gga
CHICKEN:GGAAFFX.2522.3.S1_S_AT similar to hypothetical protein LOC255104 gga
HU35KSUBB:RC_R06078_AT transmembrane and coiled-coil domains 4 hs
U133_X3P:HS2.18331.2.A1_3P_A_AT transmembrane and coiled-coil domains 4 hs
U133_X3P:HS.18331.0.A1_3P_AT transmembrane and coiled-coil domains 4 hs
HG-U133_PLUS_2:1560292_A_AT transmembrane and coiled-coil domains 4 hs
HG-U133_PLUS_2:227007_AT transmembrane and coiled-coil domains 4 hs
HG-U133B:227007_AT transmembrane and coiled-coil domains 4 hs
HG-U95B:59025_AT transmembrane and coiled-coil domains 4 hs
HG-U133_PLUS_2:233107_AT Transmembrane and coiled-coil domains 4 hs
HG-U133B:233107_AT Transmembrane and coiled-coil domains 4 hs
U133_X3P:HS.287744.0.S1_3P_X_AT Transmembrane and coiled-coil domains 4 hs
HG-U133_PLUS_2:235738_AT Transmembrane and coiled-coil domains 4 hs
HG-U133B:235738_AT Transmembrane and coiled-coil domains 4 hs
HG-U95B:46411_AT Transmembrane and coiled-coil domains 4 hs
HU35KSUBC:RC_N21548_AT Transmembrane and coiled-coil domains 4 hs
U133_X3P:HS.43018.0.A1_3P_AT Transmembrane and coiled-coil domains 4 hs
MOUSE430_2:1431810_A_AT ransmembrane and coiled-coil domains 4 mm
MOE430A:1431810_A_AT ransmembrane and coiled-coil domains 4 mm
MOUSE430A_2:1431810_A_AT ransmembrane and coiled-coil domains 4 mm
MG-U74BV2:109318_AT ransmembrane and coiled-coil domains 4 mm
MOE430B:1447636_X_AT ransmembrane and coiled-coil domains 4 mm
MOUSE430_2:1447636_X_AT ransmembrane and coiled-coil domains 4 mm
RAE230B:1391818_AT similar to RIKEN cDNA 4632413C14 rn
RAT230_2:1391818_AT similar to RIKEN cDNA 4632413C14 rn
RG-U34B:RC_AI059095_AT similar to RIKEN cDNA 4632413C14 rn
Protein Similarities
Method ID Description E-Value
blast AAA68758 Hypothetical protein F35D11.3 [Caenorhabditis elegans] ref|NP_494812.2| F35D11.3 [Caenorhabditis elegans] 0.0
blast CAE67869 Hypothetical protein CBG13461 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR007941 EMBL-EBI Protein of unknown function DUF726 1.0E-126
Pfam IPR007941 EMBL-EBI Protein of unknown function DUF726 1.0E-126
Pfam IPR007941 EMBL-EBI Protein of unknown function DUF726 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494812.2 2 218-240,255-277
F35D11.3.2 2 218-240,255-277
F35D11.3.1 2 218-240,255-277
SNAP00000013540 2 204-226,241-263
GENEFINDER00000013553 2 249-271,286-308
Sequence
Target Sequence 
>C. ELEGANS:185451_AT
tcggaaacgccatcgagtatctcatgagttttgcggtttccgtcgccatccagcaaacgc
tactggaaactgcgttggcaggtcttgtttccgctgttgcctggcctgtggctttgatgt
ccgtatcctcggtgctcgataatccatggaatgtgtgcgtttcccgtgctgctgaagttg
gggagcaattggcagaagtcttgttgagcagatctcatggaaagcgacctatcacattga
tcgggtttagtctgggtgcaagagtcatatttcattgtctcctaacaatgagcaagcgat
ctgaaagtgttggaattattgaagatgtgattctactcggagcccctgtaacagcttctc
ctaaagaatggtcaaaggtgtgtactgtagtcagtggacgagttatcaatgggtattgtg
agactgattggttattgagattcctctaccggacaatgagcgctcagtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCGGAAACGCCATCGAGTATCTCAT 299 605 1055 Antisense
TCTCATGAGTTTTGCGGTTTCCGTC 244 613 1074 Antisense
CTGCGTTGGCAGGTCTTGTTTCCGC 346 237 1124 Antisense
TATCCTCGGTGCTCGATAATCCATG 681 655 1178 Antisense
CGTTTCCCGTGCTGCTGAAGTTGGG 11 247 1212 Antisense
TGGAAAGCGACCTATCACATTGATC 607 547 1272 Antisense
TGATCGGGTTTAGTCTGGGTGCAAG 590 565 1292 Antisense
AGTCATATTTCATTGTCTCCTAACA 310 61 1317 Antisense
GAAGATGTGATTCTACTCGGAGCCC 277 339 1375 Antisense
GGTTATTGAGATTCCTCTACCGGAC 329 505 1484 Antisense
TCTACCGGACAATGAGCGCTCAGTT 637 613 1499 Antisense
Sequence Source Affymetrix Proprietary Database