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[185448_at]

Description H04J21.3 /REP_DB=WormBase Gene ID /WP=CE11566 /TR=O61208 /GB=AAB94969.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 73      6grp_high1_10_10 > 37      4grp_high_8_8 > 49      6grp_highest_nonorm > 80      6grp_total > 17      4grp_total > 62      6grp_highest_sub > Cu      6grp_cgc > 06      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 143
1 2 3 4 range
A 300.8
(3)
P 		232.2
(0.1)
P 		250.2
(0.9)
P 		157.6
(-3)
M 		143
B 246.8
(3)
P 		202.5
(-3)
P 		202.3
(-3)
P 		231.7
(1)
P 		45
C 270.5
(2.7)
P 		275.7
(3)
P 		251.3
(1.4)
P 		182.1
(-3)
P 		94
avr 272.7
(3) 		236.8
(0.4) 		234.6
(0.2) 		190.5
(-3) 		82

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 176
1 2 3 4 5 6 range
A 196.7
(1.1)
P 		217.4
(2.4)
P 		226.5
(3)
P 		133.8
(-3)
P 		167.3
(-0.8)
P 		211.3
(2)
P 		93
B 293.7
(3)
P 		191.3
(-2.2)
P 		176.5
(-3)
P 		193.1
(-2.2)
P 		198.6
(-1.9)
P 		206.3
(-1.5)
P 		117
C 310.1
(3)
P 		202.2
(-1.4)
P 		296.8
(2.5)
P 		164.5
(-3)
P 		189.2
(-2)
P 		247.4
(0.4)
P 		146
avr 266.8
(3) 		203.6
(-0.7) 		233.3
(1) 		163.8
(-3) 		185
(-1.8) 		221.7
(0.4) 		103
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 86
1 2 3 4 range
A 109.5
(3)
P 		50.4
(-1.1)
P 		65.4
(-0.1)
P 		23.1
(-3)
M 		86
B 78.7
(3)
P 		50
(-2.4)
P 		46.9
(-3)
P 		49.4
(-2.5)
P 		32
C 89.6
(3)
P 		79.3
(2)
P 		87.6
(2.8)
P 		30.7
(-3)
P 		59
avr 92.6
(3) 		59.9
(-0.4) 		66.6
(0.3) 		34.4
(-3) 		58

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 82
1 2 3 4 5 6 range
A 36.3
(-2.7)
P 		77.3
(3)
P 		49.3
(-0.9)
P 		33.9
(-3)
P 		50.5
(-0.7)
P 		51.4
(-0.6)
P 		43
B 102.2
(3)
P 		63.7
(-0.9)
P 		42.9
(-3)
P 		51.2
(-2.2)
P 		80.7
(0.8)
P 		76.9
(0.4)
P 		59
C 115
(3)
P 		61.7
(-0.9)
P 		75.5
(0.1)
P 		33.1
(-3)
P 		61.2
(-0.9)
P 		82.8
(0.6)
P 		82
avr 84.5
(3) 		67.6
(0.8) 		55.9
(-0.8) 		39.4
(-3) 		64.1
(0.3) 		70.4
(1.1) 		45
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185448_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7599
Sequence Type Exemplar sequence
Representative Public ID H04J21.3 NCBI
Target Description H04J21.3 /REP_DB=WormBase Gene ID /WP=CE11566 /TR=O61208 /GB=AAB94969.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027461(11), NM_065139(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027461 NCBI Caenorhabditis elegans Gamma-tubulin Interacting Protein family member (gip-1) (gip-1) mRNA, complete cds. 11/11 None
NM_065139 NCBI Caenorhabditis elegans Gamma-tubulin Interacting Protein family member (gip-1) (gip-1) mRNA, complete cds. 11/11 None
SNAP00000032511 ENSEMBL cdna:SNAP chromosome:CEL140:III:2347583:2361901:1 11/11 None
GENEFINDER00000032517 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2347583:2361901:1 11/11 None
H04J21.3a ENSEMBL cdna:known chromosome:CEL140:III:2347577:2362034:1 gene:H04J21.3 11/11 None
H04J21.3b ENSEMBL cdna:known chromosome:CEL140:III:2347579:2362040:1 gene:H04J21.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000032512 10/11 Negative Strand Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
175355 3 2 NM_001027461 185448_at (11)
NM_065139 185448_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2350317-2361901(+) 100.0 100.0
Public Domain and Genome References
Ensembl H04J21.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB94969 Gamma-tubulin interacting protein protein 1, isoform a [Caenorhabditis elegans] ref|NP_001022632.1| Gamma-tubulin Interacting Protein family member (gip-1) [Caenorhabditis elegans] gb|AAM34494.1| gamma-tubulin ring protein GRIP-1 [Caenorhabditis elegans] 0.0
blast AAN63399 Gamma-tubulin interacting protein protein 1, isoform b [Caenorhabditis elegans] ref|NP_497540.3| Gamma-tubulin Interacting Protein family member (gip-1) [Caenorhabditis elegans] 0.0
blast C88397 protein H04J21.3 [imported] - Caenorhabditis elegans 0.0
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497540.3 1 938-960
H04J21.3b 1 938-960
Sequence
Target Sequence 
>C. ELEGANS:185448_AT
gccacgtgtcttctgaatatgctcgcccacgtgttttccatcataaattccacgttgctc
cgtgtccgatgccacgtttctgtgcaattggctcgatttttggcccgttttttggcggcg
atcgacgaaaaatgtgtcgacgtggatgacgtcattgctgagcaccggcgatttgtgcat
cgtgcgacgtgtgtcgtttttctcaaaaattcggatcaaatcgaaaaagagctcgccact
cttgtccgttccacgtttgatgctcaagaattcacgcaggaattcggagccacttggcgt
gacgtcatcgagagcactg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCCACGTGTCTTCTGAATATGCTCG 672 279 1702 Antisense
TCCATCATAAATTCCACGTTGCTCC 398 591 1738 Antisense
TCCGATGCCACGTTTCTGTGCAATT 519 593 1766 Antisense
CAATTGGCTCGATTTTTGGCCCGTT 510 177 1786 Antisense
GGATGACGTCATTGCTGAGCACCGG 271 513 1845 Antisense
GCACCGGCGATTTGTGCATCGTGCG 480 315 1863 Antisense
GCGACGTGTGTCGTTTTTCTCAAAA 575 289 1885 Antisense
GAAAAAGAGCTCGCCACTCTTGTCC 319 355 1924 Antisense
TCCGTTCCACGTTTGATGCTCAAGA 166 595 1946 Antisense
ACGCAGGAATTCGGAGCCACTTGGC 184 87 1975 Antisense
TGGCGTGACGTCATCGAGAGCACTG 634 541 1996 Antisense
Sequence Source Affymetrix Proprietary Database