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[185447_at]

Description F08D12.9 /REP_DB=WormBase Gene ID /WP=CE09242 /TR=P91232 /GB=AAB37931.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 56      4grp_total > 10      6grp_cgc > 46      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 67
1 2 3 4 range
A 31.9
(-2.2)
A 		33.9
(-2)
A 		23.6
(-3)
A 		85.2
(3)
A 		62
B 29.7
(-0.9)
A 		19.5
(-3)
A 		31.9
(-0.5)
A 		48.9
(3)
A 		29
C 18.4
(-3)
A 		35.2
(0.3)
A 		20.2
(-2.6)
A 		48.7
(3)
A 		30
avr 26.7
(-2.7) 		29.5
(-2.3) 		25.2
(-3) 		60.9
(3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 71
1 2 3 4 5 6 range
A 36.7
(-0.3)
A 		4.2
(-3)
A 		41.4
(0.1)
A 		9.3
(-2.6)
A 		5.6
(-2.9)
A 		75.4
(3)
A 		71
B 17.8
(-2.5)
A 		39.2
(1.4)
A 		24.5
(-1.3)
A 		16.7
(-2.7)
A 		15.1
(-3)
A 		48.2
(3)
A 		33
C 6.2
(-3)
A 		49.9
(3)
A 		38.9
(1.5)
A 		15.4
(-1.7)
A 		17.8
(-1.4)
A 		48.6
(2.8)
A 		44
avr 20.2
(-2) 		31.1
(-0.5) 		34.9
(-0) 		13.8
(-2.9) 		12.8
(-3) 		57.4
(3) 		45
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 11.6
(2.1)
A 		7.4
(-1.9)
A 		6.2
(-3)
A 		12.5
(3)
A 		6
B 9.5
(2)
A 		4.8
(-3)
A 		7.4
(-0.2)
A 		10.4
(3)
A 		6
C 6.1
(-3)
A 		10.1
(3)
A 		7
(-1.7)
A 		8.2
(0.1)
A 		4
avr 9.1
(0.8) 		7.4
(-2.1) 		6.9
(-3) 		10.4
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 6.8
(-1.1)
A 		1.5
(-3)
A 		9
(-0.3)
A 		2.4
(-2.7)
A 		1.7
(-2.9)
A 		18.3
(3)
A 		17
B 6.2
(-2.2)
A 		13
(0.8)
A 		5.9
(-2.3)
A 		4.4
(-3)
A 		6.1
(-2.3)
A 		18
(3)
A 		14
C 2.3
(-3)
A 		15.2
(2.5)
A 		9.9
(0.3)
A 		3.1
(-2.7)
A 		5.7
(-1.5)
A 		16.3
(3)
A 		14
avr 5.1
(-2.2) 		9.9
(-0.2) 		8.3
(-0.9) 		3.3
(-3) 		4.5
(-2.5) 		17.5
(3) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185447_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4669
Sequence Type Exemplar sequence
Representative Public ID F08D12.9 NCBI
Target Description F08D12.9 /REP_DB=WormBase Gene ID /WP=CE09242 /TR=P91232 /GB=AAB37931.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061957(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061957 NCBI Caenorhabditis elegans F08D12.9 (F08D12.9) mRNA, complete cds. 11/11 None
SNAP00000023715 ENSEMBL cdna:SNAP chromosome:CEL140:II:2771174:2773612:-1 11/11 None
GENEFINDER00000023730 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2771174:2772240:-1 11/11 None
F08D12.9 ENSEMBL cdna:known chromosome:CEL140:II:2771174:2772240:-1 gene:F08D12.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2771172-2772239(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol sdz-10
Ensembl F08D12.9
Entrez Gene 184179 Entrez gene
SwissProt P91232 EMBL-EBI
WormBase CE09242 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB37931 Hypothetical protein F08D12.9 [Caenorhabditis elegans] ref|NP_494358.1| F08D12.9 [Caenorhabditis elegans] 0.0
blast AAB37930 Hypothetical protein F08D12.10 [Caenorhabditis elegans] ref|NP_494359.1| F08D12.10 [Caenorhabditis elegans] 0.0
blast AAB37930 Hypothetical protein F08D12.10 [Caenorhabditis elegans] ref|NP_494359.1| F08D12.10 [Caenorhabditis elegans] 1.0E-118
blast AAB37934 Hypothetical protein F08D12.11 [Caenorhabditis elegans] ref|NP_494360.1| F08D12.11 [Caenorhabditis elegans] 9.0E-52
Protein Domains
Database ID Description E-Value
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 3.2E-8
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 3.4E-7
Sequence
Target Sequence 
>C. ELEGANS:185447_AT
tacatgttccctctggctaattcaagacagataagcaaagttttaaatggtttgaaagtg
aaaaacatggatttctggaactacacagaagatggagtatttaacatcagaagcaactta
gagattgttgccttaactctcgcattccccgactcagagcatttctctgaattgtctact
cgaaggattctttcggaaaaccagacaaacatgattcatcattcgatgccatttatggca
gaaatgggagtcgaccgggttttgtattcatgtattactgtgaacgacctcttagtgaac
aactgcacacaactcaccatatcaaggtctatattaacttccaaggacatgaacattttt
cttaagcactggatcaatggatccatcaagacactagaattcttggaactgcagacgtat
gtccaaacccagtggaatatgaagatcttgttgaaagacattgatcataaaatcccgtgt
agggtggagaatggagtgccacgaggagctaaaatgtttagaattccacggatcgattcg
tcaaaccttttcatgacttatatcgtac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TACATGTTCCCTCTGGCTAATTCAA 404 643 421 Antisense
AGATTGTTGCCTTAACTCTCGCATT 701 63 542 Antisense
GCATTTCTCTGAATTGTCTACTCGA 220 309 579 Antisense
AGTCGACCGGGTTTTGTATTCATGT 281 61 669 Antisense
TGTATTACTGTGAACGACCTCTTAG 404 561 691 Antisense
GACCTCTTAGTGAACAACTGCACAC 147 383 706 Antisense
GCACACAACTCACCATATCAAGGTC 214 317 725 Antisense
GCAGACGTATGTCCAAACCCAGTGG 560 289 831 Antisense
GGAGTGCCACGAGGAGCTAAAATGT 591 515 913 Antisense
GGATCGATTCGTCAAACCTTTTCAT 86 511 950 Antisense
AACCTTTTCATGACTTATATCGTAC 579 145 964 Antisense
Sequence Source Affymetrix Proprietary Database