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[185445_s_at]

Description C27A12.6 /REP_DB=WormBase Gene ID /WP=CE08415 /TR=O01963 /GB=AAB93643.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 78      6grp_high1_10_10 > 87      4grp_high_8_8 > 42      6grp_highest_nonorm > 91      6grp_total > 87      4grp_total > 54      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 132
1 2 3 4 range
A 160.8
(3)
P 		127
(0.2)
P 		103.5
(-1.7)
P 		87.6
(-3)
P 		73
B 177.5
(3)
P 		103.4
(-2.7)
A 		99.4
(-3)
A 		112
(-2)
P 		78
C 145.3
(3)
P 		135.2
(2.4)
P 		121.3
(1.6)
P 		45.1
(-3)
A 		100
avr 161.2
(3) 		121.9
(0) 		108.1
(-1) 		81.6
(-3) 		80

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 242
1 2 3 4 5 6 range
A 143.6
(-2.9)
P 		140.1
(-3)
P 		181.8
(-1.3)
P 		228.4
(0.5)
P 		227.4
(0.5)
P 		290.4
(3)
P 		150
B 195.6
(-2.2)
P 		200.9
(-2.1)
P 		179.6
(-2.7)
P 		167.5
(-3)
P 		260.5
(-0.4)
P 		381.8
(3)
P 		214
C 218.2
(-2.3)
P 		236.8
(-1.7)
P 		197.5
(-3)
P 		201.2
(-2.9)
P 		281.2
(-0.2)
P 		379.5
(3)
P 		182
avr 185.8
(-3) 		192.6
(-2.8) 		186.3
(-3) 		199
(-2.5) 		256.4
(-0.4) 		350.6
(3) 		165
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 51
1 2 3 4 range
A 58.6
(3)
P 		27.6
(-1.1)
P 		27.1
(-1.1)
P 		12.8
(-3)
P 		46
B 56.6
(3)
P 		25.5
(-2.6)
A 		23
(-3)
A 		23.9
(-2.8)
P 		34
C 48.1
(3)
P 		38.9
(1.6)
P 		42.3
(2.1)
P 		7.6
(-3)
A 		41
avr 54.4
(3) 		30.7
(-0.6) 		30.8
(-0.6) 		14.8
(-3) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 116
1 2 3 4 5 6 range
A 26.5
(-3)
P 		49.8
(0.2)
P 		39.6
(-1.2)
P 		57.9
(1.3)
P 		68.6
(2.7)
P 		70.7
(3)
P 		44
B 68.1
(-1.5)
P 		66.9
(-1.6)
P 		43.6
(-3)
P 		44.4
(-3)
P 		105.8
(0.8)
P 		142.4
(3)
P 		99
C 80.9
(-0.2)
P 		72.3
(-0.8)
P 		50.3
(-2.3)
P 		40.5
(-3)
P 		91
(0.5)
P 		127
(3)
P 		87
avr 58.5
(-1.8) 		63
(-1.4) 		44.5
(-3) 		47.6
(-2.7) 		88.5
(0.8) 		113.4
(3) 		69
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 185445_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2283
Sequence Type Exemplar sequence
Representative Public ID C27A12.6 NCBI
Target Description C27A12.6 /REP_DB=WormBase Gene ID /WP=CE08415 /TR=O01963 /GB=AAB93643.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059346(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059346 NCBI Caenorhabditis elegans C27A12.6 (C27A12.6) mRNA, complete cds. 11/11 None
SNAP00000016771 ENSEMBL cdna:SNAP chromosome:CEL140:I:6062230:6063955:-1 11/11 None
GENEFINDER00000016777 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6062230:6063955:-1 11/11 None
C27A12.6 ENSEMBL cdna:known chromosome:CEL140:I:6061477:6063956:-1 gene:C27A12.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6062229-6063955(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C27A12.6
Entrez Gene 172282 Entrez gene
SwissProt O01963 EMBL-EBI
WormBase CE08415 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:258230_S_AT zinc finger protein-related at
Protein Similarities
Method ID Description E-Value
blast AAB93643 Hypothetical protein C27A12.6 [Caenorhabditis elegans] ref|NP_491747.1| C27A12.6 [Caenorhabditis elegans] 0.0
blast AAB93645 Hypothetical protein C27A12.8 [Caenorhabditis elegans] ref|NP_491749.2| ARI (ubiquitin ligase Ariadne) homolog family member (ari-1) [Caenorhabditis elegans] 0.0
blast AAB93644 Hypothetical protein C27A12.7a [Caenorhabditis elegans] ref|NP_491748.1| C27A12.7a [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002867 EMBL-EBI Zn-finger, cysteine-rich C6HC 1.9E-30
Pfam IPR002867 EMBL-EBI Zn-finger, cysteine-rich C6HC 7.7E-4
Sequence
Target Sequence 
>C. ELEGANS:185445_S_AT
tgctctgtacaaattctggctgccgatacgagttctgttggatgtgccttgagccatgga
ccaaacacggataccaatatgcctgcaacggatatgatgagactgctgtcaaaaaccctc
aggacgctcaggaaatatcacgtgccaacttgaagcgatatcttttctacttcaatcggt
acatggggcacgaacagagtttgcagcttgagggaaagctcaatataaaagttgcgaaga
aaatggaacagatggagaatatgtccatgtcatggattgaagttcaattcctgcgaaaag
ctgtcgatattctatcagaatgccgtcgtactttgatgtatacatatgcatttgcatttt
atttgaagaaagataataattcgattatttttgaatcaaatcaagcaaatctcgaaatgg
aaaccgaacagttatccggattcttggagagagatcttgaagatgaagatttagttactc
ttaagcaaaaggtccaagacaaatatcgctacgtagaacaacgccgcaaagtgcttttag
atcattgcgctga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCTCTGTACAAATTCTGGCTGCCG 305 581 899 Antisense
GCTGCCGATACGAGTTCTGTTGGAT 105 297 917 Antisense
GTGCCTTGAGCCATGGACCAAACAC 609 477 942 Antisense
GAGACTGCTGTCAAAAACCCTCAGG 458 393 997 Antisense
AAAACCCTCAGGACGCTCAGGAAAT 511 131 1010 Antisense
ATATCACGTGCCAACTTGAAGCGAT 471 19 1033 Antisense
GATATCTTTTCTACTTCAATCGGTA 513 425 1055 Antisense
ATTCTATCAGAATGCCGTCGTACTT 506 11 1207 Antisense
GAAACCGAACAGTTATCCGGATTCT 694 357 1318 Antisense
ATCGCTACGTAGAACAACGCCGCAA 88 37 1403 Antisense
AAGTGCTTTTAGATCATTGCGCTGA 514 157 1427 Antisense
Sequence Source Affymetrix Proprietary Database