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[185441_at]

Description H20J04.7 /REP_DB=WormBase Gene ID /WP=CE20972 /TR=Q9N5L8 /GB=AAF39885.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 81      4grp_total > 26      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 55
1 2 3 4 range
A 13.6
(-2.7)
A 		33.3
(1.9)
A 		12.2
(-3)
A 		38.1
(3)
A 		26
B 4.7
(-3)
A 		14.1
(3)
A 		12.1
(1.7)
A 		6.6
(-1.8)
A 		9
C 4.3
(-3)
A 		43.9
(1.3)
A 		28.7
(-0.3)
A 		59.5
(3)
P 		55
avr 7.5
(-3) 		30.4
(2.1) 		17.7
(-0.8) 		34.7
(3) 		27

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 52
1 2 3 4 5 6 range
A 2.2
(-3)
A 		14.9
(0)
A 		19.2
(1.1)
A 		8.5
(-1.5)
A 		27.3
(3)
A 		3.6
(-2.7)
A 		25
B 24.7
(-0.4)
A 		11
(-2)
A 		15.8
(-1.4)
A 		53.6
(3)
A 		35
(0.8)
P 		2.1
(-3)
A 		52
C 23.8
(1.2)
A 		28.1
(2.1)
A 		32.4
(3)
A 		30.8
(2.7)
A 		13.9
(-0.9)
A 		3.9
(-3)
A 		29
avr 16.9
(-0) 		18
(0.2) 		22.5
(1.2) 		31
(3) 		25.4
(1.8) 		3.2
(-3) 		28
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 5
(-0.3)
A 		7.2
(3)
A 		3.2
(-3)
A 		5.6
(0.6)
A 		4
B 1.5
(-2.7)
A 		3.5
(3)
A 		2.8
(1)
A 		1.4
(-3)
A 		2
C 1.4
(-3)
A 		12.6
(3)
A 		10
(1.6)
A 		10
(1.6)
P 		11
avr 2.6
(-3) 		7.8
(3) 		5.3
(0.1) 		5.7
(0.6) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 0.4
(-3)
A 		5.3
(0.7)
A 		4.2
(-0.1)
A 		2.2
(-1.6)
A 		8.3
(3)
A 		0.9
(-2.6)
A 		8
B 8.6
(0.5)
A 		3.6
(-1.7)
A 		3.8
(-1.7)
A 		14.2
(3)
A 		14.2
(3)
P 		0.8
(-3)
A 		13
C 8.8
(3)
A 		8.6
(2.8)
A 		8.2
(2.5)
A 		6.2
(0.9)
A 		4.5
(-0.4)
A 		1.3
(-3)
A 		8
avr 5.9
(0.7) 		5.8
(0.6) 		5.4
(0.3) 		7.5
(1.9) 		9
(3) 		1
(-3) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185441_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4635
Sequence Type Exemplar sequence
Representative Public ID H20J04.7 NCBI
Target Description H20J04.7 /REP_DB=WormBase Gene ID /WP=CE20972 /TR=Q9N5L8 /GB=AAF39885.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062367(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062367 NCBI Caenorhabditis elegans H20J04.7 (H20J04.7) mRNA, complete cds. 11/11 None
GENEFINDER00000017497 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4353215:4355311:-1 11/11 None
H20J04.7 ENSEMBL cdna:known chromosome:CEL140:II:4353215:4355311:-1 gene:H20J04.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000017484 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4353213-4355310(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl H20J04.7
Entrez Gene 186757 Entrez gene
SwissProt Q9N5L8 EMBL-EBI
WormBase CE20972 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF39885 Hypothetical protein H20J04.7 [Caenorhabditis elegans] ref|NP_494768.1| H20J04.7 [Caenorhabditis elegans] 1.0E-172
blast AAF39885 Hypothetical protein H20J04.7 [Caenorhabditis elegans] ref|NP_494768.1| H20J04.7 [Caenorhabditis elegans] 1.0E-162
blast NP_504960 C50E3.7 [Caenorhabditis elegans] gb|AAB04882.1| Hypothetical protein C50E3.7 [Caenorhabditis elegans] 1.0E-25
blast NP_504960 C50E3.7 [Caenorhabditis elegans] gb|AAB04882.1| Hypothetical protein C50E3.7 [Caenorhabditis elegans] 4.0E-24
blast CAA22112 Hypothetical protein Y75B8A.34 [Caenorhabditis elegans] ref|NP_499606.1| Y75B8A.34 [Caenorhabditis elegans] 6.0E-24
blast CAA22112 Hypothetical protein Y75B8A.34 [Caenorhabditis elegans] ref|NP_499606.1| Y75B8A.34 [Caenorhabditis elegans] 5.0E-20
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494768.1 6 41-63,76-98,121-143,150-172,206-228,249-271
H20J04.7 6 41-63,76-98,121-143,150-172,206-228,249-271
GENEFINDER00000017497 6 42-64,71-89,109-131,138-160,194-216,237-259
Sequence
Target Sequence 
>C. ELEGANS:185441_AT
gtcgttaaaccaattgttctgctttttttctaaaacttggaatcatcgaatatttggcaa
cgtgcaaattgttattcttattctattcagtgcatctttttctattactattggaatact
tggtttgagaaatttcaaaataaaacgaatatttatcttgtggttgggattcttcgaaaa
cccctggaacggacacggcgatatttacagtgataagagtatccatcgcatgtttcattt
gtttccagtagcatcaataatttgttatatgattttgtactatttgaagagacagaaaaa
taaaaaggttcttataaataccacaaatatgtctagagaggagaaaagtgttcatataga
aatacttatcacatctattttatatttaattataataattccatacgacatccttatgaa
gacaaaatatttcgaacaagattctgaggaaggcgatgctcgtttccatttcgcatcaac
atttcaaattttcaatcatttcccggaaatatccatgccattactgaatctgtactttag
tggaatattcagaaataaaattgcaccgggacctgagcatgggaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCGTTAAACCAATTGTTCTGCTTT 441 473 411 Antisense
ATTCTTATTCTATTCAGTGCATCTT 532 11 484 Antisense
GTGCATCTTTTTCTATTACTATTGG 157 481 500 Antisense
GGAACGGACACGGCGATATTTACAG 581 525 596 Antisense
GAGTATCCATCGCATGTTTCATTTG 164 399 627 Antisense
GTTTCATTTGTTTCCAGTAGCATCA 30 451 642 Antisense
GAGGAAGGCGATGCTCGTTTCCATT 327 403 856 Antisense
TTTCCATTTCGCATCAACATTTCAA 598 669 873 Antisense
CATTTCCCGGAAATATCCATGCCAT 135 215 907 Antisense
TCCATGCCATTACTGAATCTGTACT 118 589 922 Antisense
TTGCACCGGGACCTGAGCATGGGAA 225 703 971 Antisense
Sequence Source Affymetrix Proprietary Database