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[185439_at]

Description H20J04.1 /REP_DB=WormBase Gene ID /WP=CE23811 /TR=Q9N5L7 /GB=AAF39887.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 97      4grp_total > 43      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 47
1 2 3 4 range
A 15.9
(-0.9)
A 		38.2
(3)
A 		6.1
(-2.6)
A 		3.5
(-3)
A 		35
B 6.2
(-2.3)
A 		23.2
(3)
A 		14.1
(0.2)
A 		3.9
(-3)
A 		19
C 31
(-1.1)
A 		50.5
(3)
A 		29
(-1.5)
A 		22.1
(-3)
A 		28
avr 17.7
(-1.3) 		37.3
(3) 		16.4
(-1.6) 		9.8
(-3) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 56
1 2 3 4 5 6 range
A 6.2
(-2.6)
A 		3.8
(-3)
A 		3.6
(-3)
A 		4.2
(-2.9)
A 		20
(-0.7)
A 		45.7
(3)
A 		42
B 5.2
(-2.9)
A 		4.5
(-3)
A 		4.4
(-3)
A 		30.6
(0.1)
A 		34.2
(0.5)
A 		55
(3)
A 		51
C 2.7
(-3)
A 		20.8
(-1)
A 		44.7
(1.5)
A 		8.2
(-2.4)
A 		53.5
(2.5)
A 		58.2
(3)
A 		56
avr 4.7
(-3) 		9.7
(-2.4) 		17.6
(-1.4) 		14.3
(-1.8) 		35.9
(0.9) 		53
(3) 		48
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 5.8
(1.1)
A 		8.3
(3)
A 		1.6
(-2.2)
A 		0.5
(-3)
A 		8
B 2
(-1.5)
A 		5.7
(3)
A 		3.3
(0.1)
A 		0.8
(-3)
A 		5
C 10.3
(0.7)
A 		14.5
(3)
A 		10.1
(0.6)
A 		3.7
(-3)
A 		11
avr 6
(0.3) 		9.5
(3) 		5
(-0.5) 		1.7
(-3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 1.1
(-2.8)
A 		1.3
(-2.7)
A 		0.8
(-3)
A 		1.1
(-2.8)
A 		6
(0)
A 		11.1
(3)
A 		10
B 1.8
(-2.8)
A 		1.5
(-2.9)
A 		1.1
(-3)
A 		8.1
(-0.8)
A 		13.9
(1)
A 		20.5
(3)
A 		19
C 1
(-3)
A 		6.3
(-1.3)
A 		11.4
(0.4)
A 		1.7
(-2.8)
A 		17.3
(2.3)
A 		19.5
(3)
A 		19
avr 1.3
(-3) 		3
(-2.4) 		4.4
(-1.8) 		3.6
(-2.1) 		12.4
(1.2) 		17
(3) 		16
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185439_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4824
Sequence Type Exemplar sequence
Representative Public ID H20J04.1 NCBI
Target Description H20J04.1 /REP_DB=WormBase Gene ID /WP=CE23811 /TR=Q9N5L7 /GB=AAF39887.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062368(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062368 NCBI Caenorhabditis elegans H20J04.1 (H20J04.1) mRNA, complete cds. 11/11 None
SNAP00000035721 ENSEMBL cdna:SNAP chromosome:CEL140:II:4358120:4358809:1 11/11 None
GENEFINDER00000035734 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4358120:4358809:1 11/11 None
H20J04.1 ENSEMBL cdna:known chromosome:CEL140:II:4355627:4358809:1 gene:H20J04.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4355663-4358808(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl H20J04.1
Entrez Gene 173770 Entrez gene
SwissProt Q9N5L7 EMBL-EBI
WormBase CE23811 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF39887 Hypothetical protein H20J04.1 [Caenorhabditis elegans] ref|NP_494769.1| H20J04.1 [Caenorhabditis elegans] 0.0
blast AAF39887 Hypothetical protein H20J04.1 [Caenorhabditis elegans] ref|NP_494769.1| H20J04.1 [Caenorhabditis elegans] 1.0E-109
blast CAE57880 Hypothetical protein CBG00925 [Caenorhabditis briggsae] 4.0E-49
blast T15308 hypothetical protein B0286.2 - Caenorhabditis elegans 1.0E-34
blast T15308 hypothetical protein B0286.2 - Caenorhabditis elegans 1.0E-33
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494769.1 1 802-824
H20J04.1 1 802-824
SNAP00000035721 1 172-194
GENEFINDER00000035734 1 172-194
Sequence
Target Sequence 
>C. ELEGANS:185439_AT
gaaactggaaatgctctttgtctgaaagtggtgaacacgagttccgaagatgtcccacgg
aatgaatttttgaagaaattggatgttctgaatacaacaactcatcgaatcaaggatctt
cttgctcatgctttgaaactcaaaaaaagatatacagacgtgtcaaatacggatttattg
catgcaagattccttgtcgataaccttgtgaaccttccaaatccagaatataaagttgaa
gattggacaaaatttgttgatatcttgaatggaacaaatatggcaactgcatccactgaa
atctctgaattctccgaagcaatcgaatccacttttgatctggactttactgctcatcag
actgaaattattggtacttattggaatcttcgtgcattgttgagacttcatagatcactt
gtggctattgatcatgtttcacaaaaatccttctgggaacgttacaatcattggattcag
ttgtcgatgctcgttggtattctcagtttaattaatatcgttgtcctggctctaatca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAACTGGAAATGCTCTTTGTCTGA 667 357 1948 Antisense
AACACGAGTTCCGAAGATGTCCCAC 508 135 1981 Antisense
ATCTTCTTGCTCATGCTTTGAAACT 552 31 2063 Antisense
AGATTCCTTGTCGATAACCTTGTGA 695 59 2134 Antisense
AACCTTGTGAACCTTCCAAATCCAG 690 143 2149 Antisense
GCAACTGCATCCACTGAAATCTCTG 95 321 2230 Antisense
GAAGCAATCGAATCCACTTTTGATC 705 339 2263 Antisense
TGATCTGGACTTTACTGCTCATCAG 661 565 2283 Antisense
GAGACTTCATAGATCACTTGTGGCT 285 393 2349 Antisense
GATGCTCGTTGGTATTCTCAGTTTA 108 415 2433 Antisense
AATATCGTTGTCCTGGCTCTAATCA 447 171 2461 Antisense
Sequence Source Affymetrix Proprietary Database