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[185436_at]

Description F35H10.5 /REP_DB=WormBase Gene ID /WP=CE04505 /TR=Q20071 /GB=AAA81681.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 79      6grp_high1_10_10 > 97      4grp_highest_8_8 > 14      4grp_high_8_8 > 23      6grp_highest_nonorm > 92      6grp_total > 96      4grp_total > 16      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 168
1 2 3 4 range
A 129.4
(-3)
P 		156.2
(-1.9)
P 		234.3
(1.3)
P 		275
(3)
P 		146
B 150.5
(-3)
P 		151
(-2.9)
P 		199.1
(3)
P 		188.4
(1.7)
P 		49
C 158.6
(-1.8)
P 		195.1
(-0.5)
P 		123.9
(-3)
P 		291.5
(3)
P 		168
avr 146.2
(-3) 		167.4
(-1.8) 		185.8
(-0.7) 		251.6
(3) 		105

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 346
1 2 3 4 5 6 range
A 150.9
(-2.4)
P 		131.6
(-3)
P 		236
(0.1)
P 		271.1
(1.1)
P 		250
(0.5)
P 		336.7
(3)
P 		205
B 152.9
(-2.4)
P 		170.2
(-2)
P 		120.5
(-3)
P 		182.4
(-1.8)
P 		358.4
(1.6)
P 		429.2
(3)
P 		309
C 146.6
(-3)
P 		166
(-2.6)
P 		173.3
(-2.5)
P 		266.5
(-0.8)
P 		320.3
(0.3)
P 		466.5
(3)
P 		320
avr 150.1
(-3) 		155.9
(-2.9) 		176.6
(-2.4) 		240
(-0.9) 		309.6
(0.7) 		410.8
(3) 		261
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 27
1 2 3 4 range
A 47.1
(-0.1)
P 		33.9
(-3)
P 		61.2
(3)
P 		40.3
(-1.6)
P 		27
B 48
(3)
P 		37.3
(-3)
P 		46.1
(1.9)
P 		40.2
(-1.4)
P 		11
C 52.5
(1.3)
P 		56.1
(3)
P 		43.2
(-3)
P 		49.2
(-0.2)
P 		13
avr 49.2
(2.2) 		42.4
(-3) 		50.2
(3) 		43.2
(-2.4) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 132
1 2 3 4 5 6 range
A 27.8
(-3)
P 		46.8
(-0.9)
P 		51.4
(-0.4)
P 		68.8
(1.5)
P 		75.4
(2.3)
P 		82
(3)
P 		54
B 53.2
(-1.9)
P 		56.6
(-1.7)
P 		29.3
(-3)
P 		48.3
(-2.1)
P 		145.6
(2.3)
P 		160
(3)
P 		131
C 54.3
(-2.5)
P 		50.6
(-2.7)
P 		44.1
(-3)
P 		53.6
(-2.5)
P 		103.6
(0.2)
P 		156.2
(3)
P 		112
avr 45.1
(-2.8) 		51.3
(-2.4) 		41.6
(-3) 		56.9
(-2) 		108.2
(1.4) 		132.7
(3) 		91
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185436_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9453
Sequence Type Exemplar sequence
Representative Public ID F35H10.5 NCBI
Target Description F35H10.5 /REP_DB=WormBase Gene ID /WP=CE04505 /TR=Q20071 /GB=AAA81681.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068997(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068997 NCBI Caenorhabditis elegans F35H10.5 (F35H10.5) mRNA, complete cds. 11/11 None
SNAP00000039425 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8300760:8301081:1 11/11 None
GENEFINDER00000039435 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8300760:8301081:1 11/11 None
F35H10.5 ENSEMBL cdna:known chromosome:CEL140:IV:8300760:8301081:1 gene:F35H10.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8300765-8301087(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F35H10.5
Entrez Gene 185330 Entrez gene
SwissProt Q20071 EMBL-EBI
WormBase CE04505 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA81681 Hypothetical protein F35H10.5 [Caenorhabditis elegans] ref|NP_501398.1| F35H10.5 [Caenorhabditis elegans] 3.0E-35
blast CAE58453 Hypothetical protein CBG01590 [Caenorhabditis briggsae] 2.0E-29
Sequence
Target Sequence 
>C. ELEGANS:185436_AT
aatgcacatcaaacagtctacagtaagatcgatgttgattgtggctcggcgaggcaagaa
acggttgaacttggatccggatgcactagctgttctcacagctcttatcaatttgcttgc
acaggagtcggtagctcgagccgcacagtccgctgccaacactggaagccgccacgtcac
aaaagaacact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATGCACATCAAACAGTCTACAGTA 48 165 18 Antisense
ACAGTCTACAGTAAGATCGATGTTG 275 107 30 Antisense
ATCGATGTTGATTGTGGCTCGGCGA 628 35 45 Antisense
TGTGGCTCGGCGAGGCAAGAAACGG 525 553 57 Antisense
GAAACGGTTGAACTTGGATCCGGAT 63 339 75 Antisense
TTGGATCCGGATGCACTAGCTGTTC 108 709 88 Antisense
CTGTTCTCACAGCTCTTATCAATTT 105 239 107 Antisense
GCTCTTATCAATTTGCTTGCACAGG 125 301 118 Antisense
AATTTGCTTGCACAGGAGTCGGTAG 328 173 127 Antisense
CACAGGAGTCGGTAGCTCGAGCCGC 445 193 137 Antisense
AGCCGCCACGTCACAAAAGAACACT 141 83 184 Antisense
Sequence Source Affymetrix Proprietary Database