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[185432_at]

Description Y50D7A.8 /REP_DB=WormBase Gene ID /WP=CE26145 /TR=Q9N3K5 /GB=AAF59643.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 51      6grp_high1_10_10 > 15      4grp_highest_8_8 > 55      4grp_high_8_8 > 19      6grp_highest_nonorm > 90      6grp_total > 16      4grp_total > 29      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 124
1 2 3 4 range
A 158.8
(2.2)
P 		168.2
(3)
P 		97
(-3)
P 		149.2
(1.4)
P 		71
B 172.7
(3)
P 		137
(-1)
P 		119.8
(-3)
P 		150.5
(0.5)
P 		53
C 164.6
(0)
P 		128.1
(-1.9)
P 		107.9
(-3)
P 		221.1
(3)
P 		113
avr 165.4
(2.2) 		144.4
(0.3) 		108.2
(-3) 		173.6
(3) 		65

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 99
1 2 3 4 5 6 range
A 193.5
(1.4)
P 		211.9
(3)
P 		144.2
(-3)
P 		152.8
(-2.2)
P 		163.6
(-1.3)
P 		153.8
(-2.1)
P 		68
B 175
(3)
P 		167.4
(2)
P 		148.1
(-0.7)
P 		159.1
(0.8)
P 		134.1
(-2.6)
P 		131
(-3)
P 		44
C 214.1
(3)
P 		175.5
(0.7)
P 		153.4
(-0.7)
P 		115
(-3)
P 		153.6
(-0.7)
P 		145
(-1.2)
P 		99
avr 194.2
(3) 		184.9
(1.9) 		148.6
(-2.3) 		142.3
(-3) 		150.4
(-2.1) 		143.3
(-2.9) 		52
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 36
1 2 3 4 range
A 57.8
(3)
P 		36.5
(-0.6)
P 		25.3
(-2.4)
P 		21.8
(-3)
P 		36
B 55.1
(3)
P 		33.8
(-1.7)
P 		27.8
(-3)
P 		32.1
(-2.1)
P 		27
C 54.5
(3)
P 		36.8
(-3)
P 		37.6
(-2.7)
P 		37.3
(-2.8)
P 		18
avr 55.8
(3) 		35.7
(-1.7) 		30.2
(-3) 		30.4
(-3) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 56
1 2 3 4 5 6 range
A 35.7
(-2.4)
P 		75.4
(3)
P 		31.4
(-3)
P 		38.8
(-2)
P 		49.4
(-0.5)
P 		37.5
(-2.2)
P 		44
B 60.9
(3)
P 		55.7
(1.7)
P 		36
(-3)
P 		42.1
(-1.5)
P 		54.5
(1.5)
P 		48.8
(0.1)
P 		25
C 79.3
(3)
P 		53.6
(0.3)
P 		39.1
(-1.3)
P 		23.1
(-3)
P 		49.7
(-0.2)
P 		48.5
(-0.3)
P 		56
avr 58.6
(2.3) 		61.6
(3) 		35.5
(-2.8) 		34.7
(-3) 		51.2
(0.7) 		44.9
(-0.7) 		27
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185432_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8322
Sequence Type Exemplar sequence
Representative Public ID Y50D7A.8 NCBI
Target Description Y50D7A.8 /REP_DB=WormBase Gene ID /WP=CE26145 /TR=Q9N3K5 /GB=AAF59643.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064785
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064785 NCBI Caenorhabditis elegans Y50D7A.8 (Y50D7A.8) mRNA, complete cds. A 
Y50D7A.8 ENSEMBL cdna:known chromosome:CEL140:III:335955:343240:-1 gene:Y50D7A.8 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:333671-336946(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y50D7A.8
Entrez Gene 175194 Entrez gene
SwissProt Q9N3K5 EMBL-EBI
WormBase CE30029 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF59643 Hypothetical protein Y50D7A.8 [Caenorhabditis elegans] ref|NP_497186.2| Y50D7A.8 [Caenorhabditis elegans] 1.0E-144
blast C88379 protein W07B3.1 [imported] - Caenorhabditis elegans pir||T33657 hypothetical protein W07B3.1 - Caenorhabditis elegans (fragment) 3.0E-24
blast CAE69500 Hypothetical protein CBG15708 [Caenorhabditis briggsae] 3.0E-16
Sequence
Target Sequence 
>C. ELEGANS:185432_AT
gaagcagtcactcaggattggcatgttggatcctccacgtcatcaattccaccgcattat
acgcaccaccatcccactgcagaagacatggattatgatgatattggtgaagaagctcta
acccaagacgaaataaagctaaaatgtctgaaagcacggcgagccgagcgagcaagagcc
cgatatcatcgaatgtcagaggagaagcgtcgagaacagaatgctcgacgggccctgatg
ctccgaaaagcccgtgcccgagacgatgaacttctgaaaattgctgaaacaacgccgatg
gatgaaattgaggaatcgattgcggcggcgattcgagatgctcaacaaagaagagcacga
aaagctgaacaggcgagaagcaagtatcgacgaatgactgatgaggagaaacggagattt
aatgcagctagggatgcacataaacggggcaaaaaggaatccgaaacttcactgcctcca
gctcctccaacagacccagaactatcacaatcaccactcgaagaaccatctacgtcatca
cctggtgacgtcatagatcctgatgacgcgtcaagcttcttctat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGCAGTCACTCAGGATTGGCATG 636 339 352 Antisense
TCATCAATTCCACCGCATTATACGC 24 621 391 Antisense
ATGTCTGAAAGCACGGCGAGCCGAG 241 49 495 Antisense
GAGCGAGCAAGAGCCCGATATCATC 462 385 517 Antisense
GTGCCCGAGACGATGAACTTCTGAA 469 477 605 Antisense
GATTGCGGCGGCGATTCGAGATGCT 557 433 669 Antisense
AAGGAATCCGAAACTTCACTGCCTC 259 159 805 Antisense
CAGACCCAGAACTATCACAATCACC 663 203 842 Antisense
AATCACCACTCGAAGAACCATCTAC 238 171 860 Antisense
ATCACCTGGTGACGTCATAGATCCT 138 29 888 Antisense
TGATGACGCGTCAAGCTTCTTCTAT 93 567 912 Antisense
Sequence Source Affymetrix Proprietary Database