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[185429_at]

Description C25E10.7 /REP_DB=WormBase Gene ID /WP=CE06869 /TR=Q18156 /GB=AAA92312.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 82      6grp_high1_10_10 > 38      4grp_high_8_8 > 03      6grp_highest_nonorm > 90      6grp_total > 27      4grp_total > 21      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 185
1 2 3 4 range
A 98.2
(-0.8)
P 		62.8
(-2)
P 		31
(-3)
A 		216
(3)
P 		185
B 77.5
(-0.8)
P 		82.2
(-0.3)
M 		58.6
(-3)
A 		110.5
(3)
P 		52
C 109.8
(3)
P 		69.2
(-2)
A 		61.2
(-3)
P 		64.7
(-2.6)
P 		49
avr 95.2
(0.4) 		71.4
(-1.4) 		50.3
(-3) 		130.4
(3) 		80

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 75
1 2 3 4 5 6 range
A 104.7
(3)
P 		51.8
(-2.9)
P 		57.8
(-2.2)
P 		51
(-3)
P 		54.3
(-2.6)
P 		83.4
(0.6)
P 		54
B 106.9
(2.2)
P 		92.4
(0.6)
P 		59.1
(-3)
P 		58.9
(-3)
P 		114.6
(3)
P 		101.4
(1.6)
P 		56
C 82.9
(1.8)
P 		93.4
(3)
P 		87.3
(2.3)
P 		69.5
(0.3)
P 		39.5
(-3)
P 		64.5
(-0.2)
P 		54
avr 98.2
(3) 		79.2
(0) 		68.1
(-1.7) 		59.8
(-3) 		69.5
(-1.5) 		83.1
(0.6) 		38
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 28
1 2 3 4 range
A 35.8
(3)
P 		13.6
(-1.8)
P 		8.1
(-3)
A 		31.6
(2.1)
P 		28
B 24.7
(3)
P 		20.3
(0.6)
M 		13.6
(-3)
A 		23.5
(2.4)
P 		11
C 36.3
(3)
P 		19.9
(-0.9)
A 		21.3
(-0.5)
P 		10.9
(-3)
P 		25
avr 32.3
(3) 		17.9
(-1.8) 		14.3
(-3) 		22
(-0.4) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 34
1 2 3 4 5 6 range
A 19.3
(2.2)
P 		18.4
(1.5)
P 		12.6
(-3)
P 		12.9
(-2.8)
P 		16.4
(-0)
P 		20.3
(3)
P 		8
B 37.2
(1.3)
P 		30.7
(0)
P 		14.3
(-3)
P 		15.6
(-2.8)
P 		46.6
(3)
P 		37.8
(1.4)
P 		32
C 30.7
(3)
P 		28.5
(2.3)
P 		22.2
(0.2)
P 		14
(-2.6)
P 		12.8
(-3)
P 		21.6
(-0.1)
P 		18
avr 29.1
(3) 		25.9
(1.7) 		16.4
(-2.1) 		14.2
(-3) 		25.3
(1.5) 		26.6
(2) 		15
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185429_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11757
Sequence Type Exemplar sequence
Representative Public ID C25E10.7 NCBI
Target Description C25E10.7 /REP_DB=WormBase Gene ID /WP=CE06869 /TR=Q18156 /GB=AAA92312.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072943(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072943 NCBI Caenorhabditis elegans C25E10.7 (C25E10.7) mRNA, complete cds. 11/11 None
SNAP00000038737 ENSEMBL cdna:SNAP chromosome:CEL140:V:9052713:9053566:-1 11/11 None
GENEFINDER00000038748 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9052713:9053566:-1 11/11 None
C25E10.7 ENSEMBL cdna:known chromosome:CEL140:V:9052713:9053566:-1 gene:C25E10.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9052713-9053567(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C25E10.7
Entrez Gene 182891 Entrez gene
SwissProt Q18156 EMBL-EBI
WormBase CE06869 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA92312 Hypothetical protein C25E10.7 [Caenorhabditis elegans] ref|NP_505344.1| C25E10.7 [Caenorhabditis elegans] 1.0E-89
blast CAE72084 Hypothetical protein CBG19171 [Caenorhabditis briggsae] 1.0E-63
blast CAE72086 Hypothetical protein CBG19174 [Caenorhabditis briggsae] 2.0E-33
Protein Domains
Database ID Description E-Value
Pfam IPR002919 EMBL-EBI Trypsin inhibitor-like, cysteine-rich TIL region 1.1E-20
Pfam IPR002919 EMBL-EBI Trypsin inhibitor-like, cysteine-rich TIL region 1.7E-21
Sequence
Target Sequence 
>C. ELEGANS:185429_AT
cacaatggatcacaccaggagccccgtttttaccagaatgtgggaaaaaccaaaaacgag
tggcttgtggttatgattgtgaaccacaatgcggatttgatccaactgtatgcagtttgg
agtgtaaaccgaacgcttgtgtttgtaaggatggctacgtgaggaacacaaaaaatgatt
gtgtccgtcgacttgaatgcactgcagaaacatccagatgtccagaagatgaagtattcc
agacttgtggaacactttgtcaaccaacttgtgatgacccgtacccaacatcttgtgaac
atgatcgatgcatccggaatgtctgccgatgccttccaggattggtcagg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CACAATGGATCACACCAGGAGCCCC 223 193 26 Antisense
GCGGATTTGATCCAACTGTATGCAG 578 293 116 Antisense
GGAGTGTAAACCGAACGCTTGTGTT 354 515 144 Antisense
TCCGTCGACTTGAATGCACTGCAGA 158 595 209 Antisense
GCACTGCAGAAACATCCAGATGTCC 284 315 224 Antisense
GTGGAACACTTTGTCAACCAACTTG 19 487 272 Antisense
TCAACCAACTTGTGATGACCCGTAC 316 629 285 Antisense
CCCGTACCCAACATCTTGTGAACAT 101 261 303 Antisense
GTGAACATGATCGATGCATCCGGAA 67 483 320 Antisense
GCATCCGGAATGTCTGCCGATGCCT 353 309 335 Antisense
CCGATGCCTTCCAGGATTGGTCAGG 518 263 351 Antisense
Sequence Source Affymetrix Proprietary Database