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[185427_s_at]

Description H21P03.3B /REP_DB=WormBase Gene ID /WP=CE23816 /TR=Q9U3D4 /GB=CAB54269.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 88      6grp_high1_10_10 > 89      4grp_highest_8_8 > 39      4grp_high_8_8 > 12      6grp_highest_nonorm > 84      6grp_total > 89      4grp_total > 12      6grp_highest_sub > Da      6grp_cgc > 79      6grp_high1_sub > Cu      6grp_cgc_sub > Da      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 931
1 2 3 4 range
A 941.9
(-0.2)
P 		688
(-2.8)
P 		669.1
(-3)
P 		1257.1
(3)
P 		588
B 823.4
(-1.6)
P 		774.4
(-1.9)
P 		599.6
(-3)
P 		1530.2
(3)
P 		931
C 781.3
(-1.8)
P 		712.4
(-2.6)
P 		682.6
(-3)
P 		1176.1
(3)
P 		494
avr 848.9
(-1.2) 		724.9
(-2.3) 		650.4
(-3) 		1321.1
(3) 		671

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 529
1 2 3 4 5 6 range
A 471.6
(-2.9)
P 		611.3
(-1)
P 		504.8
(-2.4)
P 		464.8
(-3)
P 		813.7
(1.9)
P 		895.9
(3)
P 		431
B 520
(-1.9)
P 		603
(-1)
P 		423.2
(-3)
P 		612.4
(-0.9)
P 		777.1
(1)
P 		952.1
(3)
P 		529
C 544.3
(-1)
P 		563.2
(-0.7)
P 		527.8
(-1.4)
P 		449.7
(-3)
P 		729.2
(2.8)
P 		739.8
(3)
P 		290
avr 512
(-2.6) 		592.5
(-1.3) 		485.3
(-3) 		509
(-2.6) 		773.3
(1.6) 		862.6
(3) 		377
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 204
1 2 3 4 range
A 342.9
(3)
P 		149.4
(-3)
P 		174.9
(-2.2)
P 		184
(-1.9)
P 		194
B 262.6
(1)
P 		191.3
(-1.3)
P 		138.9
(-3)
P 		326.1
(3)
P 		187
C 258.6
(3)
P 		204.8
(-2.4)
P 		237.9
(0.9)
P 		198.3
(-3)
P 		60
avr 288
(3) 		181.8
(-3) 		183.9
(-2.9) 		236.1
(0.1) 		106

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 268
1 2 3 4 5 6 range
A 86.9
(-3)
P 		217.4
(1.9)
P 		109.9
(-2.1)
P 		117.9
(-1.8)
P 		245.5
(3)
P 		218.1
(2)
P 		159
B 180.9
(-1.1)
P 		200.7
(-0.7)
P 		102.8
(-3)
P 		162.2
(-1.6)
P 		315.7
(2.1)
P 		355
(3)
P 		252
C 201.8
(1.2)
P 		171.8
(0.1)
P 		134.3
(-1.3)
P 		90.4
(-3)
P 		236
(2.6)
P 		247.7
(3)
P 		157
avr 156.5
(-1.4) 		196.6
(0.1) 		115.7
(-3) 		123.5
(-2.7) 		265.7
(2.7) 		273.6
(3) 		158
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 185427_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10023
Sequence Type Exemplar sequence
Representative Public ID H21P03.3B NCBI
Target Description H21P03.3B /REP_DB=WormBase Gene ID /WP=CE23816 /TR=Q9U3D4 /GB=CAB54269.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069768(11), NM_069767(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069768 NCBI Caenorhabditis elegans SphingoMyelin Synthase family member (sms-1) (sms-1) mRNA, complete cds. 11/11 None
NM_069767 NCBI Caenorhabditis elegans SphingoMyelin Synthase family member (sms-1) (sms-1) mRNA, complete cds. 11/11 None
SNAP00000044460 ENSEMBL cdna:SNAP chromosome:CEL140:IV:11483200:11487872:-1 9/11 None
GENEFINDER00000044465 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:11483200:11498138:-1 9/11 None
H21P03.3a ENSEMBL cdna:known chromosome:CEL140:IV:11482693:11487965:-1 gene:H21P03.3 11/11 A 
H21P03.3b ENSEMBL cdna:known chromosome:CEL140:IV:11482975:11498138:-1 gene:H21P03.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:11482980-11498144(-) 100.0 100.0
Public Domain and Genome References
Ensembl H21P03.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB09114 Hypothetical protein H21P03.3a [Caenorhabditis elegans] ref|NP_502169.1| SphingoMyelin Synthase family member (sms-1) [Caenorhabditis elegans] 0.0
blast CAB54269 Hypothetical protein H21P03.3b [Caenorhabditis elegans] ref|NP_502168.1| SphingoMyelin Synthase family member (sms-1) [Caenorhabditis elegans] sp|Q9U3D4|SMS1_CAEEL Putative phosphatidylcholine:ceramide cholinephosphotransferase 1 (Sphingomyelin synthase 1) 0.0
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_502169.1 6 146-168,188-210,217-239,263-280,285-307,317-339
NP_502168.1 6 186-208,228-250,257-279,303-320,325-347,357-379
H21P03.3a 6 146-168,188-210,217-239,263-280,285-307,317-339
H21P03.3b 6 186-208,228-250,257-279,303-320,325-347,357-379
SNAP00000044460 6 144-166,186-208,215-237,261-278,283-305,315-337
GENEFINDER00000044465 6 158-180,200-222,229-251,275-292,297-319,329-351
Sequence
Target Sequence 
>C. ELEGANS:185427_S_AT
aagatttcgaatgctcgccacgtttcggtgaaaatgccacgttctcgttgattgtgatgc
gaggtgtttggagcatgttcgggctcggtctcaacttattcgacaatcaaaaagtcgttt
tgtgtggagactacatttacagtggacacacacttgtgctcgtcgtatcggctcttttca
ttggagaatactctcctcgacgattctacattcttcactggctgtcatggttagtgtgtt
ccgttggtgtgatctttctagttctatcacacggacactacactattgatgtgatacttt
catatttcgcttgtacacgagtattctgggcatatcatacacaggctgcacatccatcca
ttcggctttctgtacaaaatcatcaagccaaagagttttggttcccattgttgcgatggt
ttgaaggagacatacggcgacctgttccacgaagattcgattgtccaatctcgtattcac
aaacgcccgtagattattctttcttaccttggttttcatttttctttcaatccaacccga
tgatgttcccgggtgctttcacgttttatcacactc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGATTTCGAATGCTCGCCACGTTT 490 155 872 Antisense
GGCTCGGTCTCAACTTATTCGACAA 115 539 953 Antisense
TGGACACACACTTGTGCTCGTCGTA 345 547 1014 Antisense
GTCGTATCGGCTCTTTTCATTGGAG 57 473 1033 Antisense
TCTACATTCTTCACTGGCTGTCATG 143 615 1076 Antisense
GTTCCGTTGGTGTGATCTTTCTAGT 280 439 1109 Antisense
ATCATACACAGGCTGCACATCCATC 555 27 1205 Antisense
GAGTTTTGGTTCCCATTGTTGCGAT 280 399 1264 Antisense
GCGACCTGTTCCACGAAGATTCGAT 259 289 1308 Antisense
CAATCCAACCCGATGATGTTCCCGG 548 169 1399 Antisense
GGTGCTTTCACGTTTTATCACACTC 583 501 1423 Antisense
Sequence Source Affymetrix Proprietary Database