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[185423_at]

Description F52C9.8B /REP_DB=WormBase Gene ID /WP=CE04621 /TR=Q20650 /GB=AAA81058.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 26      4grp_total > 31      6grp_cgc > 24      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 112
1 2 3 4 range
A 226
(3)
P 		156.4
(-1.8)
A 		139.8
(-3)
A 		221
(2.7)
A 		86
B 202.9
(3)
P 		113.8
(-3)
A 		173.6
(1)
A 		131.3
(-1.8)
A 		89
C 213.5
(3)
A 		126.2
(-3)
A 		150.4
(-1.3)
A 		173.4
(0.2)
A 		87
avr 214.1
(3) 		132.1
(-3) 		154.6
(-1.4) 		175.2
(0.2) 		82

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 177
1 2 3 4 5 6 range
A 199.1
(1.4)
A 		234
(3)
A 		150.5
(-0.8)
A 		149.1
(-0.9)
A 		102.7
(-3)
A 		187.6
(0.9)
P 		131
B 176.4
(3)
P 		147.7
(0.5)
A 		144.8
(0.3)
A 		111.3
(-2.6)
A 		107
(-3)
A 		141.9
(0)
A 		69
C 280
(3)
P 		198.4
(0.1)
A 		163.9
(-1.1)
A 		108.9
(-3)
A 		141.3
(-1.9)
A 		114.1
(-2.8)
A 		171
avr 218.5
(3) 		193.4
(1.5) 		153.1
(-0.9) 		123.1
(-2.6) 		117
(-3) 		147.9
(-1.2) 		102
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 54
1 2 3 4 range
A 82.3
(3)
P 		34
(-2.8)
A 		36.6
(-2.5)
A 		32.4
(-3)
A 		50
B 64.7
(3)
P 		28.1
(-3)
A 		40.2
(-1)
A 		28
(-3)
A 		37
C 70.7
(3)
A 		36.3
(-2)
A 		52.4
(0.4)
A 		29.2
(-3)
A 		42
avr 72.6
(3) 		32.8
(-2.6) 		43.1
(-1.1) 		29.9
(-3) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 82
1 2 3 4 5 6 range
A 36.7
(-2.3)
A 		83.2
(3)
A 		32.8
(-2.8)
A 		37.8
(-2.2)
A 		31
(-3)
A 		45.7
(-1.3)
P 		52
B 61.4
(3)
P 		49.2
(0.7)
A 		35.2
(-1.9)
A 		29.5
(-3)
A 		43.5
(-0.4)
A 		52.9
(1.4)
A 		32
C 103.8
(3)
P 		60.5
(-0.2)
A 		41.7
(-1.5)
A 		21.9
(-3)
A 		45.7
(-1.3)
A 		38.2
(-1.8)
A 		82
avr 67.3
(3) 		64.3
(2.5) 		36.6
(-1.9) 		29.7
(-3) 		40.1
(-1.3) 		45.6
(-0.5) 		38
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185423_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6316
Sequence Type Exemplar sequence
Representative Public ID F52C9.8B NCBI
Target Description F52C9.8B /REP_DB=WormBase Gene ID /WP=CE04621 /TR=Q20650 /GB=AAA81058.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065735(10), NM_065734(11), NM_065736(9), NM_171864(11), NM_171119(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065735 NCBI Caenorhabditis elegans PolyQ (poly glutamine tract) toxicity Enhancer family member (pqe-1) (pqe-1) mRNA, complete cds. 10/11 A 
NM_065734 NCBI Caenorhabditis elegans PolyQ (poly glutamine tract) toxicity Enhancer family member (pqe-1) (pqe-1) mRNA, complete cds. 11/11 A 
NM_065736 NCBI Caenorhabditis elegans PolyQ (poly glutamine tract) toxicity Enhancer family member (pqe-1) (pqe-1) mRNA, complete cds. 9/11 None
NM_171864 NCBI Caenorhabditis elegans PolyQ (poly glutamine tract) toxicity Enhancer family member (pqe-1) (pqe-1) mRNA, complete cds. 11/11 A 
NM_171119 NCBI Caenorhabditis elegans PolyQ (poly glutamine tract) toxicity Enhancer family member (pqe-1) (pqe-1) mRNA, complete cds. 11/11 A 
SNAP00000014751 ENSEMBL cdna:SNAP chromosome:CEL140:III:5311217:5316931:1 10/11 None
GENEFINDER00000014757 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5311217:5317275:1 10/11 None
F52C9.8a ENSEMBL cdna:known chromosome:CEL140:III:5311203:5316134:1 gene:F52C9.8 9/11 A 
F52C9.8c ENSEMBL cdna:known chromosome:CEL140:III:5311203:5321689:1 gene:F52C9.8 11/11 A 
F52C9.8b ENSEMBL cdna:known chromosome:CEL140:III:5311217:5321328:1 gene:F52C9.8 11/11 A 
F52C9.8d ENSEMBL cdna:known chromosome:CEL140:III:5315570:5321328:1 gene:F52C9.8 11/11 A 
F52C9.8e ENSEMBL cdna:known chromosome:CEL140:III:5315633:5321328:1 gene:F52C9.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
175731 6 5 NM_065734 185403_s_at (11), 185423_at (11), 193167_s_at (11)
NM_065735 185423_at (10), 193167_s_at (11)
NM_065736 185403_s_at (11), 185423_at (9)
NM_171119 185423_at (11), 193167_s_at (11)
NM_171120 193167_s_at (11)
NM_171864 185423_at (11), 193167_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5311516-5316231(+) 97.51 100.0
Public Domain and Genome References
Ensembl F52C9.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM45368 Polyq (poly glutamine tract) toxicity enhancer protein 1, isoform c [Caenorhabditis elegans] ref|NP_498136.2| PolyQ (poly glutamine tract) toxicity Enhancer family member (pqe-1) [Caenorhabditis elegans] 0.0
blast AAM45367 Polyq (poly glutamine tract) toxicity enhancer protein 1, isoform b [Caenorhabditis elegans] ref|NP_498135.2| PolyQ (poly glutamine tract) toxicity Enhancer family member (pqe-1) [Caenorhabditis elegans] sp|Q10124|PQE1_CAEEL PolyQ enhancer protein 0.0
blast AAM45369 Polyq (poly glutamine tract) toxicity enhancer protein 1, isoform d [Caenorhabditis elegans] ref|NP_741134.1| PolyQ (poly glutamine tract) toxicity Enhancer family member (pqe-1) [Caenorhabditis elegans] 0.0
blast AAM45366 Polyq (poly glutamine tract) toxicity enhancer protein 1, isoform a [Caenorhabditis elegans] ref|NP_498137.2| PolyQ (poly glutamine tract) toxicity Enhancer family member (pqe-1) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006055 EMBL-EBI Exonuclease 4.7E-35
Pfam IPR006055 EMBL-EBI Exonuclease 4.7E-35
Pfam IPR006055 EMBL-EBI Exonuclease 4.7E-35
Pfam IPR006055 EMBL-EBI Exonuclease 4.7E-35
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000014757 1 1266-1288
Sequence
Target Sequence 
>C. ELEGANS:185423_AT
tgaataagcgaccgacatcctcagcgtcggtggattcaaacgatgatggggtgcacattc
cggcgaaaagaatggctcacgcgagttctgtaccagggccttcgagatcaaaaccaccga
tgatcggagctgtcaagaatcgtccaaatcacactgaaatgcttgacaaaaggaataaag
agagcgaggaaaagaggcgaaaagatagagatgaacttgaacggcttcggaacaagaaac
acaccaccgaagaggaaaagataaagatggctcggctccaaaacgcactgaaagttgttg
gaaaagcggcaggattgaaagcaactgtaaagaaggagttaactggaagccctgccaaaa
agcaaaaaccggcaccgctgtgccga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGAATAAGCGACCGACATCCTCAGC 23 577 3095 Antisense
GACATCCTCAGCGTCGGTGGATTCA 69 371 3108 Antisense
AACGATGATGGGGTGCACATTCCGG 439 145 3133 Antisense
AGAATGGCTCACGCGAGTTCTGTAC 27 75 3163 Antisense
GTACCAGGGCCTTCGAGATCAAAAC 534 451 3184 Antisense
GATCGGAGCTGTCAAGAATCGTCCA 480 417 3216 Antisense
GATGAACTTGAACGGCTTCGGAACA 316 411 3304 Antisense
GAACAAGAAACACACCACCGAAGAG 314 351 3324 Antisense
GATAAAGATGGCTCGGCTCCAAAAC 310 419 3354 Antisense
GGAGTTAACTGGAAGCCCTGCCAAA 18 517 3429 Antisense
GCAAAAACCGGCACCGCTGTGCCGA 33 319 3456 Antisense
Sequence Source Affymetrix Proprietary Database