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[185421_at]

Description Y53F4B.10 /REP_DB=WormBase Gene ID /WP=CE22398 /TR=Q9NAC2 /GB=CAB61079.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 07      4grp_total > 01      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 158
1 2 3 4 range
A 35.5
(-2.1)
A 		11.5
(-3)
A 		21.7
(-2.6)
A 		167.9
(3)
A 		156
B 33.9
(-0.9)
A 		9.9
(-3)
A 		55.1
(1)
A 		77.6
(3)
A 		68
C 59.4
(3)
A 		47.9
(-0)
A 		36.7
(-3)
A 		58.6
(2.8)
A 		23
avr 42.9
(-1.5) 		23.1
(-3) 		37.8
(-1.9) 		101.4
(3) 		78

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 115
1 2 3 4 5 6 range
A 128.8
(3)
A 		44.6
(-1.6)
A 		20.1
(-3)
A 		55.4
(-1.1)
A 		75.4
(0.1)
A 		45.9
(-1.6)
A 		109
B 127.9
(3)
A 		51.6
(-1.8)
A 		72.8
(-0.5)
A 		32.3
(-3)
A 		35.7
(-2.8)
A 		32.8
(-3)
A 		96
C 81.9
(1.3)
A 		109
(3)
A 		49.5
(-0.8)
A 		34.2
(-1.7)
A 		14.1
(-3)
A 		37
(-1.6)
A 		95
avr 112.9
(3) 		68.4
(-0.6) 		47.5
(-2.3) 		40.6
(-2.8) 		41.7
(-2.7) 		38.6
(-3) 		74
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 22
1 2 3 4 range
A 12.9
(-0.2)
A 		2.5
(-3)
A 		5.7
(-2.1)
A 		24.6
(3)
A 		22
B 10.8
(0.6)
A 		2.4
(-3)
A 		12.8
(1.4)
A 		16.5
(3)
A 		14
C 19.7
(3)
A 		13.8
(-0.6)
A 		12.8
(-1.2)
A 		9.9
(-3)
A 		10
avr 14.5
(1.6) 		6.2
(-3) 		10.4
(-0.7) 		17
(3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 40
1 2 3 4 5 6 range
A 23.7
(3)
A 		15.9
(0.6)
A 		4.4
(-3)
A 		14
(-0)
A 		22.7
(2.7)
A 		11.2
(-0.9)
A 		19
B 44.5
(3)
A 		17.2
(-1.6)
A 		17.7
(-1.5)
A 		8.6
(-3)
A 		14.5
(-2)
A 		12.2
(-2.4)
A 		36
C 30.3
(2.4)
A 		33.2
(3)
A 		12.6
(-1.3)
A 		6.9
(-2.5)
A 		4.6
(-3)
A 		12.4
(-1.4)
A 		29
avr 32.8
(3) 		22.1
(0.2) 		11.6
(-2.5) 		9.8
(-3) 		13.9
(-1.9) 		11.9
(-2.5) 		23
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185421_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4033
Sequence Type Exemplar sequence
Representative Public ID Y53F4B.10 NCBI
Target Description Y53F4B.10 /REP_DB=WormBase Gene ID /WP=CE22398 /TR=Q9NAC2 /GB=CAB61079.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064693(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064693 NCBI Caenorhabditis elegans Y53F4B.10 (Y53F4B.10) mRNA, complete cds. 11/11 None
SNAP00000043045 ENSEMBL cdna:SNAP chromosome:CEL140:II:14999703:15012515:-1 11/11 None
GENEFINDER00000043117 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:15001854:15010301:-1 11/11 None
Y53F4B.10 ENSEMBL cdna:known chromosome:CEL140:II:15001985:15003400:-1 gene:Y53F4B.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:15001981-15003397(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y53F4B.10
Entrez Gene 190212 Entrez gene
SwissProt Q9NAC2 EMBL-EBI
WormBase CE22398 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB61079 Hypothetical protein Y53F4B.10 [Caenorhabditis elegans] ref|NP_497094.1| Y53F4B.10 [Caenorhabditis elegans] 0.0
blast CAB61080 Hypothetical protein Y53F4B.9 [Caenorhabditis elegans] ref|NP_497095.1| Y53F4B.9 [Caenorhabditis elegans] 0.0
blast CAE73538 Hypothetical protein CBG21002 [Caenorhabditis briggsae] 0.0
blast AAC48140 Hypothetical protein C41D11.6 [Caenorhabditis elegans] ref|NP_491376.1| C41D11.6 [Caenorhabditis elegans] 4.0E-35
Sequence
Target Sequence 
>C. ELEGANS:185421_AT
attttgacgcctctaacagtgcctacaaaactacttggtgaatcttgtcgaggggtggtt
cgagcttttctgccacaagcggccccgggcttgacgctgagctctctgaaaccaggagtt
ctatacgaagaagttagaagacgcacggctcatcttggcagtcactgggaagcgatgtcg
ccgcaagttcttgatcttgaggtggttgataatggaagtatgctatcccgacttgttgaa
gaatgcatcgatgtgaatacggaagctattgcaacattatatgaagatcgtggcactcat
cccactaaagttgaaaaggttacctgcggtctccgtaccgttactattcaatttacaatc
ggtactccatcttattccgcagctattcgaaaatggaaaaaaggaactaggtgctcgttg
gaaactgctgcgggtgatattctttctgtgttggtggagaatgctatcccattaaaatct
cgggttatcatcacagcaagca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTTTGACGCCTCTAACAGTGCCTA 459 13 841 Antisense
GGCAGTCACTGGGAAGCGATGTCGC 1 537 997 Antisense
GATGTCGCCGCAAGTTCTTGATCTT 233 409 1014 Antisense
GTATGCTATCCCGACTTGTTGAAGA 495 455 1058 Antisense
TGAAGATCGTGGCACTCATCCCACT 416 575 1122 Antisense
TGAAAAGGTTACCTGCGGTCTCCGT 603 571 1152 Antisense
GTCTCCGTACCGTTACTATTCAATT 12 471 1169 Antisense
TACAATCGGTACTCCATCTTATTCC 219 641 1194 Antisense
TCCATCTTATTCCGCAGCTATTCGA 396 591 1206 Antisense
TGCTGCGGGTGATATTCTTTCTGTG 245 583 1266 Antisense
TCTCGGGTTATCATCACAGCAAGCA 670 611 1318 Antisense
Sequence Source Affymetrix Proprietary Database