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[185416_at]

Description F35D11.4 /REP_DB=WormBase Gene ID /WP=CE02717 /TR=Q20034 /GB=AAA68750.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 45      6grp_high1_10_10 > 54      4grp_highest_8_8 > 17      4grp_high_8_8 > 61      6grp_highest_nonorm > 72      6grp_total > 54      4grp_total > 59      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 764
1 2 3 4 range
A 500.9
(-0.2)
P 		793
(2.5)
P 		842.8
(3)
P 		210.6
(-3)
P 		632
B 652.5
(0.2)
P 		853.5
(2)
P 		974.4
(3)
P 		273.2
(-3)
P 		701
C 561.4
(0.3)
P 		814.9
(3)
P 		754.8
(2.4)
P 		251.6
(-3)
P 		563
avr 571.6
(0.2) 		820.5
(2.6) 		857.3
(3) 		245.1
(-3) 		612

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 251
1 2 3 4 5 6 range
A 279.9
(-3)
P 		530.7
(3)
P 		513
(2.6)
P 		447.9
(1)
P 		346.3
(-1.4)
P 		429.3
(0.6)
P 		251
B 451.6
(1.3)
P 		505.9
(3)
P 		480.8
(2.2)
P 		319
(-3)
P 		375.8
(-1.2)
P 		347.6
(-2.1)
P 		187
C 424.2
(-1.2)
P 		389.7
(-3)
P 		478.9
(1.7)
P 		426
(-1.1)
P 		404.3
(-2.2)
P 		503.7
(3)
P 		114
avr 385.2
(-2.5) 		475.4
(2.2) 		490.9
(3) 		397.6
(-1.9) 		375.5
(-3) 		426.9
(-0.3) 		115
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 232
1 2 3 4 range
A 182.4
(1.8)
P 		172.1
(1.5)
P 		220.3
(3)
P 		30.8
(-3)
P 		190
B 208.1
(2.4)
P 		210.8
(2.5)
P 		225.8
(3)
P 		58.2
(-3)
P 		168
C 185.8
(0.9)
P 		234.3
(2.2)
P 		263.1
(3)
P 		42.4
(-3)
P 		221
avr 192.1
(1.6) 		205.7
(2) 		236.4
(3) 		43.8
(-3) 		193

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 137
1 2 3 4 5 6 range
A 51.6
(-3)
P 		188.7
(3)
P 		111.7
(-0.4)
P 		113.6
(-0.3)
P 		104.5
(-0.7)
P 		104.5
(-0.7)
P 		137
B 157.1
(2.2)
P 		168.4
(3)
P 		116.7
(-0.7)
P 		84.5
(-3)
P 		152.7
(1.9)
P 		129.6
(0.2)
P 		84
C 157.2
(2.2)
P 		118.9
(-0.6)
P 		121.9
(-0.4)
P 		85.7
(-3)
P 		130.8
(0.3)
P 		168.6
(3)
P 		83
avr 122
(-0.4) 		158.7
(3) 		116.8
(-0.9) 		94.6
(-3) 		129.3
(0.2) 		134.2
(0.7) 		64
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185416_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4486
Sequence Type Exemplar sequence
Representative Public ID F35D11.4 NCBI
Target Description F35D11.4 /REP_DB=WormBase Gene ID /WP=CE02717 /TR=Q20034 /GB=AAA68750.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062410(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062410 NCBI Caenorhabditis elegans F35D11.4 (F35D11.4) mRNA, complete cds. 11/11 None
SNAP00000013539 ENSEMBL cdna:SNAP chromosome:CEL140:II:4603421:4604053:1 11/11 None
GENEFINDER00000013555 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4603306:4604053:1 10/11 None
F35D11.4 ENSEMBL cdna:known chromosome:CEL140:II:4603414:4604098:1 gene:F35D11.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4603304-4604052(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F35D11.4
Entrez Gene 173797 Entrez gene
SwissProt Q20034 EMBL-EBI
WormBase CE32648 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1631048_AT dm
DROSGENOME1:144625_AT dm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6171 cAMP biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4016 adenylate cyclase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA68750 Hypothetical protein F35D11.4 [Caenorhabditis elegans] ref|NP_494811.2| F35D11.4 [Caenorhabditis elegans] 2.0E-94
blast AAA68750 Hypothetical protein F35D11.4 [Caenorhabditis elegans] ref|NP_494811.2| F35D11.4 [Caenorhabditis elegans] 9.0E-93
blast CAE67852 Hypothetical protein CBG13441 [Caenorhabditis briggsae] 1.0E-88
blast CAE67852 Hypothetical protein CBG13441 [Caenorhabditis briggsae] 6.0E-87
blast EAA06833 ENSANGP00000019035 [Anopheles gambiae str. PEST] ref|XP_311209.2| ENSANGP00000019035 [Anopheles gambiae str. PEST] 2.0E-34
Protein Domains
Database ID Description E-Value
Pfam IPR008172 EMBL-EBI Adenylate cyclase 1.8E-32
Pfam IPR008172 EMBL-EBI Adenylate cyclase 5.9E-33
Sequence
Target Sequence 
>C. ELEGANS:185416_AT
aacagccgactatcttgaagcagcatgacatcttttacgagtcaccgaatggccgcctga
agatgagaagcgttgaggagaacggagtggcccacacagagcttatttggtacgatagat
ctgacgtggctggcccgaagctcagtaatttcaacaaattcgacgttccatctgaagttc
ttgatgctctgaagctctccctccaatcctcaatgggagtcaaaggagaggtgaaaaaga
cgagaactctggtgctccacggtcaaacacgaattcatattgatcgcgtcgatgggctcg
gtgattttatggagttggaagtgtgcctgtcgcctgaagagactccagagcacggagaga
agatagctcatgagattcgggagctgctcgccgtgccagagaccgatttgctcac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACAGCCGACTATCTTGAAGCAGCA 358 135 161 Antisense
TTTTACGAGTCACCGAATGGCCGCC 559 671 193 Antisense
TGAGGAGAACGGAGTGGCCCACACA 617 567 234 Antisense
ACGATAGATCTGACGTGGCTGGCCC 548 93 272 Antisense
TGGCTGGCCCGAAGCTCAGTAATTT 169 545 287 Antisense
ATTCGACGTTCCATCTGAAGTTCTT 137 9 318 Antisense
AGTTCTTGATGCTCTGAAGCTCTCC 153 65 336 Antisense
TCGCGTCGATGGGCTCGGTGATTTT 310 597 444 Antisense
TGGAGTTGGAAGTGTGCCTGTCGCC 603 549 470 Antisense
TCATGAGATTCGGGAGCTGCTCGCC 414 621 528 Antisense
CCGTGCCAGAGACCGATTTGCTCAC 652 263 551 Antisense
Sequence Source Affymetrix Proprietary Database