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[185410_s_at]

Description Y113G7A.9 /REP_DB=WormBase Gene ID /WP=CE23280 /TR=Q9U2Y7 /GB=CAB60481.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 99      6grp_high1_10_10 > 99      4grp_highest_8_8 > 11      4grp_high_8_8 > 47      6grp_highest_nonorm > 75      6grp_total > 95      4grp_total > 40      6grp_cgc > 78      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S5      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 404
1 2 3 4 range
A 583.3
(-3)
P 		786.2
(0)
P 		987.2
(3)
P 		655.3
(-1.9)
P 		404
B 645.7
(-3)
P 		834.8
(0.8)
P 		942.6
(3)
P 		789.2
(-0.1)
P 		297
C 632.2
(-3)
P 		832.2
(0.9)
P 		938.3
(3)
P 		794.2
(0.2)
P 		306
avr 620.4
(-3) 		817.7
(0.5) 		956
(3) 		746.2
(-0.8) 		336

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 473
1 2 3 4 5 6 range
A 847
(0.2)
P 		686.2
(-3)
P 		907.2
(1.4)
P 		855.4
(0.3)
P 		989.4
(3)
P 		975.4
(2.7)
P 		303
B 866.4
(-3)
P 		875.4
(-2.8)
P 		866.9
(-3)
P 		950.4
(-1.3)
P 		1158.9
(3)
P 		997
(-0.3)
P 		293
C 791.7
(-2.1)
P 		846.4
(-1.2)
P 		740.4
(-3)
P 		993.7
(1.3)
P 		979.3
(1.1)
P 		1090.3
(3)
P 		350
avr 835
(-2.2) 		802.7
(-3) 		838.2
(-2.1) 		933.2
(0.3) 		1042.5
(3) 		1020.9
(2.5) 		240
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 231
1 2 3 4 range
A 212.4
(1.3)
P 		170.7
(-0.2)
P 		258.1
(3)
P 		95.9
(-3)
P 		162
B 205.9
(1.5)
P 		206.2
(1.5)
P 		218.4
(3)
P 		168.2
(-3)
P 		50
C 209.3
(-0.7)
P 		239.3
(0.3)
P 		327
(3)
P 		133.9
(-3)
P 		193
avr 209.2
(0.4) 		205.4
(0.2) 		267.8
(3) 		132.7
(-3) 		135

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 315
1 2 3 4 5 6 range
A 156.1
(-3)
P 		244.1
(0.7)
P 		197.6
(-1.3)
P 		216.9
(-0.4)
P 		298.5
(3)
P 		237.5
(0.4)
P 		142
B 301.5
(-0.9)
P 		291.4
(-1.1)
P 		210.5
(-3)
P 		251.7
(-2.1)
P 		470.8
(3)
P 		371.8
(0.7)
P 		260
C 293.5
(0.6)
P 		258.3
(-0.6)
P 		188.4
(-3)
P 		199.8
(-2.6)
P 		316.9
(1.4)
P 		365
(3)
P 		177
avr 250.4
(-1.1) 		264.6
(-0.6) 		198.8
(-3) 		222.8
(-2.1) 		362.1
(3) 		324.8
(1.6) 		163
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 185410_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13648
Sequence Type Exemplar sequence
Representative Public ID Y113G7A.9 NCBI
Target Description Y113G7A.9 /REP_DB=WormBase Gene ID /WP=CE23280 /TR=Q9U2Y7 /GB=CAB60481.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075475(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075475 NCBI Caenorhabditis elegans DeCapping Scavenger enzyme homolog family member (dcs-1) (dcs-1) mRNA, complete cds. 11/11 None
GENEFINDER00000016742 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:20085900:20088739:-1 11/11 None
Y113G7A.9 ENSEMBL cdna:known chromosome:CEL140:V:20085845:20088739:-1 gene:Y113G7A.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:20085899-20088739(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol dcs-1
Ensembl Y113G7A.9
Entrez Gene 180315 Entrez gene
SwissProt Q9U2Y7 EMBL-EBI
WormBase CE23280 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.18558.1.S1_S_AT similar to mRNA decapping enzyme cfa
DROSOPHILA_2:1632438_AT dm
DROSGENOME1:151994_AT dm
CHICKEN:GGAAFFX.703.1.S1_AT similar to HINT-5 gga
HG-U133_PLUS_2:218774_AT decapping enzyme, scavenger hs
HG-U133A:218774_AT decapping enzyme, scavenger hs
HG-U133A_2:218774_AT decapping enzyme, scavenger hs
HU35KSUBA:RC_AA491001_F_AT decapping enzyme, scavenger hs
HU35KSUBA:RC_AA491001_I_AT decapping enzyme, scavenger hs
HU35KSUBD:RC_H04142_F_AT decapping enzyme, scavenger hs
U133_X3P:G7661733_3P_AT decapping enzyme, scavenger hs
HG-U95B:54630_AT decapping enzyme, scavenger hs
HU35KSUBA:RC_AA411228_AT Decapping enzyme, scavenger hs
HU35KSUBC:RC_Z39740_AT Decapping enzyme, scavenger hs
U133_X3P:HS.22590.1.A1_3P_AT Decapping enzyme, scavenger hs
U133_X3P:HS.22590.0.A1_3P_AT Decapping enzyme, scavenger hs
HG-U133_PLUS_2:227925_AT Decapping enzyme, scavenger hs
HG-U133B:227925_AT Decapping enzyme, scavenger hs
HG-U133_PLUS_2:230999_AT Decapping enzyme, scavenger hs
HG-U133B:230999_AT Decapping enzyme, scavenger hs
HG-U95B:43011_AT Decapping enzyme, scavenger hs
HG-U95B:46960_AT Decapping enzyme, scavenger hs
MOUSE430A_2:1456571_AT decapping enzyme, scavenger mm
MOE430A:1456571_AT decapping enzyme, scavenger mm
MOUSE430_2:1456571_AT decapping enzyme, scavenger mm
MU11KSUBA:AA473067_S_AT decapping enzyme, scavenger mm
MG-U74AV2:94067_AT decapping enzyme, scavenger mm
MG-U74CV2:168872_AT decapping enzyme, scavenger mm
MOE430A:1424080_AT decapping enzyme, scavenger mm
MOUSE430A_2:1424080_AT decapping enzyme, scavenger mm
MOUSE430_2:1424080_AT decapping enzyme, scavenger mm
MOE430B:1446979_AT Decapping enzyme, scavenger (Dcps), mRNA mm
MOUSE430_2:1446979_AT Decapping enzyme, scavenger (Dcps), mRNA mm
MOE430B:1459135_AT Decapping enzyme, scavenger (Dcps), mRNA mm
MOUSE430_2:1459135_AT Decapping enzyme, scavenger (Dcps), mRNA mm
MU19KSUBA:TC19410_AT Decapping enzyme, scavenger (Dcps), mRNA mm
RG-U34C:RC_AI072775_AT mRNA decapping enzyme rn
RAE230A:1390185_AT mRNA decapping enzyme rn
RAT230_2:1390185_AT mRNA decapping enzyme rn
RAE230B:1381527_AT MRNA decapping enzyme rn
RAT230_2:1381527_AT MRNA decapping enzyme rn
YEAST_2:1772009_AT Non-essential hydrolase involved in mRNA decapping, may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p Sc
Protein Similarities
Method ID Description E-Value
blast CAB60481 Hypothetical protein Y113G7A.9 [Caenorhabditis elegans] ref|NP_507876.1| DeCapping Scavenger enzyme homolog family member (dcs-1) [Caenorhabditis elegans] gb|AAL86013.1| heat shock-like protein [Caenorhabditis elegans] 1.0E-171
blast CAE67441 Hypothetical protein CBG12933 [Caenorhabditis briggsae] 1.0E-154
blast NP_991151 mRNA decapping enzyme [Danio rerio] gb|AAH94967.1| MRNA decapping enzyme [Danio rerio] 8.0E-63
Protein Domains
Database ID Description E-Value
Pfam IPR008594 EMBL-EBI Scavenger mRNA decapping enzyme 6.1E-93
Sequence
Target Sequence 
>C. ELEGANS:185410_S_AT
gatcgtctcattgcaaaataccgtcaggaggagaaattcgtgattcgagagactccagag
ctttatgagactgttacaaggccgtatattgagaaatatcagctgaatctcaattgggtg
tacaattgcctggaaaagcgctcggaagtcgataaaatcgtgttcgaagaccccgacaat
gaaaatggcttcgtacttcttcaggatatcaaatgggacggaaaaaccttggaaaatctc
tatgttctcgctatttgtcaccgccacggcctgaaatctgtccgggatttgactggagat
gatttggagatgctgtacaacatgcgtgacaagtccctcgaggcaataaatcagaaatat
ggcctaaaaaccgatcaaatcaagtgctatttccattatcaaccgtctttttatcatctt
cacgttcattttatcaacctaaaatacgatgccccggcgagcactacaatgtcggctatc
ctgctcgatgacgtcatcaacaatttagagcttaacccggagcattataagaaatccacg
ttgacatttacccga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCGTCTCATTGCAAAATACCGTC 118 419 343 Antisense
AATTGCCTGGAAAAGCGCTCGGAAG 155 179 466 Antisense
AATCGTGTTCGAAGACCCCGACAAT 199 169 498 Antisense
AAAATGGCTTCGTACTTCTTCAGGA 347 133 524 Antisense
ATCTCTATGTTCTCGCTATTTGTCA 350 33 578 Antisense
CCACGGCCTGAAATCTGTCCGGGAT 641 273 606 Antisense
GTACAACATGCGTGACAAGTCCCTC 614 459 657 Antisense
ATCAACCGTCTTTTTATCATCTTCA 193 27 740 Antisense
TATCATCTTCACGTTCATTTTATCA 685 659 754 Antisense
GCACTACAATGTCGGCTATCCTGCT 38 317 803 Antisense
GAAATCCACGTTGACATTTACCCGA 666 361 873 Antisense
Sequence Source Affymetrix Proprietary Database