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[185407_at]

Description Y39B6B.DD /REP_DB=WormBase Gene ID /WP=CE21676 /TR=Q9NET0 /GB=CAB60935.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 4grp_high_8_8 > 42      6grp_total > 15      4grp_total > 45      6grp_cgc > 22      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 241
1 2 3 4 range
A 303.5
(3)
P 		241
(0.6)
P 		149.8
(-3)
P 		152.7
(-2.9)
A 		154
B 298.9
(3)
P 		136.8
(-1.8)
P 		150.1
(-1.5)
A 		98.3
(-3)
P 		201
C 339.5
(3)
P 		303.8
(1.8)
P 		158.2
(-3)
A 		204.4
(-1.5)
A 		181
avr 314
(3) 		227.2
(-0.2) 		152.7
(-3) 		151.8
(-3) 		162

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 316
1 2 3 4 5 6 range
A 193.7
(1.5)
A 		228.3
(3)
P 		122.3
(-1.5)
M 		148.6
(-0.4)
M 		86.5
(-3)
A 		103
(-2.3)
M 		142
B 205.9
(2.9)
P 		208
(3)
P 		127.6
(-1.3)
A 		103.1
(-2.7)
A 		96.8
(-3)
M 		113
(-2.1)
P 		111
C 402.8
(3)
P 		210.9
(-0.7)
P 		243.1
(-0)
A 		88.1
(-3)
A 		138.1
(-2)
P 		95.3
(-2.9)
M 		315
avr 267.5
(3) 		215.7
(1.1) 		164.3
(-0.8) 		113.3
(-2.7) 		107.1
(-2.9) 		103.8
(-3) 		164
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 92
1 2 3 4 range
A 110.5
(3)
P 		52.3
(-1)
P 		39.2
(-1.9)
P 		22.3
(-3)
A 		88
B 95.3
(3)
P 		33.8
(-2)
P 		34.8
(-1.9)
A 		20.9
(-3)
P 		74
C 112.4
(3)
P 		87.4
(1.1)
P 		55.1
(-1.4)
A 		34.5
(-3)
A 		78
avr 106.1
(3) 		57.8
(-0.6) 		43
(-1.7) 		25.9
(-3) 		80

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 132
1 2 3 4 5 6 range
A 35.7
(-1.9)
A 		81.2
(3)
P 		26.6
(-2.8)
M 		37.7
(-1.7)
M 		26.1
(-2.9)
A 		25.1
(-3)
M 		56
B 71.6
(3)
P 		69.2
(2.7)
P 		31
(-2.5)
A 		27.3
(-3)
A 		39.3
(-1.4)
M 		42.2
(-1)
P 		44
C 149.3
(3)
P 		64.4
(-0.9)
P 		61.9
(-1)
A 		17.7
(-3)
A 		44.7
(-1.8)
P 		31.9
(-2.4)
M 		132
avr 85.5
(3) 		71.6
(1.6) 		39.8
(-1.7) 		27.6
(-3) 		36.7
(-2.1) 		33.1
(-2.4) 		58
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185407_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19421
Sequence Type Exemplar sequence
Representative Public ID Y39B6B.DD NCBI
Target Description Y39B6B.DD /REP_DB=WormBase Gene ID /WP=CE21676 /TR=Q9NET0 /GB=CAB60935.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171605(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171605 NCBI Caenorhabditis elegans Homolog of Odr-2(Two) family member (hot-2) (hot-2) mRNA, complete cds. 9/11 None
GENEFINDER00000030708 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:19185693:19190240:1 10/11 None
Y39B6A.48 ENSEMBL cdna:known chromosome:CEL140:V:19187728:19190472:1 gene:Y39B6A.48 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
AF324054 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:19180569-19190240(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol hot-2
Ensembl Y39B6A.48
Entrez Gene 180261 Entrez gene
SwissProt Q8I0Z9 EMBL-EBI
WormBase CE36958 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAD57707 Hypothetical protein Y39B6A.48 [Caenorhabditis elegans] ref|NP_741695.2| Homolog of Odr-2(Two) family member (hot-2) [Caenorhabditis elegans] 1.0E-106
blast T45056 hypothetical protein Y39B6B.dd [imported] - Caenorhabditis elegans 1.0E-103
blast CAE73145 Hypothetical protein CBG20535 [Caenorhabditis briggsae] 3.0E-88
blast AAK01415 Ly-6-related protein HOT-2 [Caenorhabditis elegans] 7.0E-81
blast CAD57707 Hypothetical protein Y39B6A.48 [Caenorhabditis elegans] ref|NP_741695.2| Homolog of Odr-2(Two) family member (hot-2) [Caenorhabditis elegans] 2.0E-63
blast AAK01415 Ly-6-related protein HOT-2 [Caenorhabditis elegans] 2.0E-58
Protein Domains
Database ID Description E-Value
Pfam IPR010558 EMBL-EBI Caenorhabditis elegans ly-6-related 4.8E-87
Pfam IPR010558 EMBL-EBI Caenorhabditis elegans ly-6-related 1.1E-62
Sequence
Target Sequence 
>C. ELEGANS:185407_AT
atggctcagaaggtcgggggttcgacccccaagccggcaagcctccctcctcctaaaaat
tattttcttgcaggcgccggtgctccaatcccacgacaagtcgaatgttactcgtgcatg
tcgctgtcttaccagactagttggaaatacttacaaactacgtatatttatcctaaaacc
tcagaattttgagaaaatcggaaaattcgaaaaattgaagaaaaaaatttgccatttttt
tttgcaactttcgaatttttaccaattttcaaaaaatatggtcttcaccgacagatgccg
cgacccgaatagtgaacgtggaatgcctactgtaatgtgtggaagtgtatgtgtatcgct
gatggagcccgatgtggaagctggagtatttatcggcttcaaacatatccgaggatgctt
ggaccgagttttgcggcacggatttaaccaatcagcgctccgaactcaccggtttcatca
gaataaccactgccgaacgctttcccgctcagccctcttcaacccagcccggcagactga
ccccccggcactcggcgatgttcaactgtgctcgtgttacggtgaccgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGGCTCAGAAGGTCGGGGGTTCGA 475 53 48 Antisense
AAATTATTTTCTTGCAGGCGCCGGT 305 115 104 Antisense
GTCGAATGTTACTCGTGCATGTCGC 150 475 147 Antisense
GCATGTCGCTGTCTTACCAGACTAG 90 311 163 Antisense
ATGCCGCGACCCGAATAGTGAACGT 278 37 342 Antisense
GTGTATCGCTGATGGAGCCCGATGT 163 487 398 Antisense
GAGGATGCTTGGACCGAGTTTTGCG 165 403 458 Antisense
CGAACTCACCGGTTTCATCAGAATA 267 253 508 Antisense
CAGAATAACCACTGCCGAACGCTTT 440 203 526 Antisense
CACTCGGCGATGTTCAACTGTGCTC 580 197 596 Antisense
ACTGTGCTCGTGTTACGGTGACCGT 414 95 612 Antisense
Sequence Source Affymetrix Proprietary Database