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[185403_s_at]

Description F52C9.8A /REP_DB=WormBase Gene ID /WP=CE04620 /TR=Q20649 /GB=AAA81057.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 02      4grp_total > 58      6grp_cgc > 43      6grp_cgc_sub > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 104
1 2 3 4 range
A 90.3
(1.6)
A 		21.8
(-3)
A 		111.7
(3)
A 		40.3
(-1.8)
A 		90
B 21
(-2.5)
A 		108.6
(3)
A 		103.2
(2.7)
A 		12.6
(-3)
A 		96
C 66
(-0)
A 		116.4
(3)
A 		101.8
(2.1)
A 		16
(-3)
A 		100
avr 59.1
(-0.4) 		82.3
(1.3) 		105.6
(3) 		23
(-3) 		83

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 120
1 2 3 4 5 6 range
A 20.6
(-3)
A 		112.4
(3)
A 		68.1
(0.1)
A 		38.8
(-1.8)
A 		66.6
(0)
A 		78
(0.8)
A 		92
B 86.9
(1.6)
A 		106.7
(3)
A 		61.4
(-0.2)
A 		72.2
(0.6)
A 		21.3
(-3)
A 		43.4
(-1.4)
A 		85
C 140.9
(3)
A 		101.3
(1)
A 		23.4
(-3)
A 		22.9
(-3)
A 		105.6
(1.2)
A 		46.9
(-1.8)
A 		118
avr 82.8
(0.7) 		106.8
(3) 		51
(-2.4) 		44.6
(-3) 		64.5
(-1.1) 		56.1
(-1.9) 		62
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 33
1 2 3 4 range
A 32.9
(3)
A 		4.7
(-3)
A 		29.2
(2.2)
A 		5.9
(-2.7)
A 		28
B 6.7
(-2)
A 		26.8
(3)
A 		23.9
(2.3)
A 		2.7
(-3)
A 		24
C 21.8
(0.5)
A 		33.5
(2.6)
A 		35.5
(3)
A 		2.7
(-3)
A 		33
avr 20.5
(0.9) 		21.7
(1.2) 		29.5
(3) 		3.8
(-3) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 48
1 2 3 4 5 6 range
A 3.8
(-3)
A 		40
(3)
A 		14.8
(-1.2)
A 		9.8
(-2)
A 		20.1
(-0.3)
A 		19
(-0.5)
A 		36
B 30.2
(1.8)
A 		35.5
(3)
A 		14.9
(-1.6)
A 		19.1
(-0.7)
A 		8.7
(-3)
A 		16.2
(-1.3)
A 		27
C 52.2
(3)
A 		30.9
(0.3)
A 		6
(-2.8)
A 		4.6
(-3)
A 		34.2
(0.7)
A 		15.7
(-1.6)
A 		48
avr 28.7
(1.3) 		35.5
(3) 		11.9
(-2.8) 		11.2
(-3) 		21
(-0.6) 		17
(-1.6) 		24
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 185403_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6332
Sequence Type Exemplar sequence
Representative Public ID F52C9.8A NCBI
Target Description F52C9.8A /REP_DB=WormBase Gene ID /WP=CE04620 /TR=Q20649 /GB=AAA81057.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065734(11), NM_065736(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065734 NCBI Caenorhabditis elegans PolyQ (poly glutamine tract) toxicity Enhancer family member (pqe-1) (pqe-1) mRNA, complete cds. 11/11 A 
NM_065736 NCBI Caenorhabditis elegans PolyQ (poly glutamine tract) toxicity Enhancer family member (pqe-1) (pqe-1) mRNA, complete cds. 11/11 None
SNAP00000014751 ENSEMBL cdna:SNAP chromosome:CEL140:III:5311217:5316931:1 11/11 None
GENEFINDER00000014757 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5311217:5317275:1 11/11 None
F52C9.8a ENSEMBL cdna:known chromosome:CEL140:III:5311203:5316134:1 gene:F52C9.8 11/11 A 
F52C9.8b ENSEMBL cdna:known chromosome:CEL140:III:5311217:5321328:1 gene:F52C9.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
175731 6 2 NM_065734 185403_s_at (11), 185423_at (11), 193167_s_at (11)
NM_065735 185423_at (10), 193167_s_at (11)
NM_065736 185403_s_at (11), 185423_at (9)
NM_171119 185423_at (11), 193167_s_at (11)
NM_171120 193167_s_at (11)
NM_171864 185423_at (11), 193167_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5311516-5315488(+) 97.11 100.0
Public Domain and Genome References
Ensembl F52C9.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM45367 Polyq (poly glutamine tract) toxicity enhancer protein 1, isoform b [Caenorhabditis elegans] ref|NP_498135.2| PolyQ (poly glutamine tract) toxicity Enhancer family member (pqe-1) [Caenorhabditis elegans] sp|Q10124|PQE1_CAEEL PolyQ enhancer protein 0.0
blast AAM45368 Polyq (poly glutamine tract) toxicity enhancer protein 1, isoform c [Caenorhabditis elegans] ref|NP_498136.2| PolyQ (poly glutamine tract) toxicity Enhancer family member (pqe-1) [Caenorhabditis elegans] 0.0
blast AAM45369 Polyq (poly glutamine tract) toxicity enhancer protein 1, isoform d [Caenorhabditis elegans] ref|NP_741134.1| PolyQ (poly glutamine tract) toxicity Enhancer family member (pqe-1) [Caenorhabditis elegans] 0.0
blast AAM45366 Polyq (poly glutamine tract) toxicity enhancer protein 1, isoform a [Caenorhabditis elegans] ref|NP_498137.2| PolyQ (poly glutamine tract) toxicity Enhancer family member (pqe-1) [Caenorhabditis elegans] 0.0
blast T16420 hypothetical protein F52C9.8b - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006055 EMBL-EBI Exonuclease 4.7E-35
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000014757 1 1266-1288
Sequence
Target Sequence 
>C. ELEGANS:185403_S_AT
tccagagtacagtgctgcgagcccatcggaggatgaagatgatgacatcggaagctttgt
ggtcagtgataatgaggatgatgatgcagatagttttgtggttggtgatgatgaaccgat
agagtatgaggaagaagatgaggatgatatgatagaaagaagaagttcgagaaagaggag
agacactccgacaggcagcagaagcatgagaagcacttcaccgaatgatcgccgaaagtc
tcgagagactcctcccggcaacagaagcatgaggaggactagtccttccgatggcagaaa
atccagagacactccaaccgccagtagcagtatgagcagctctacgctttcctattgtaa
aaaatccaaagagactccgatgtcctatgaagaaattgagcagcagaaaaaagcgaaacg
tcagcgaaattgcaagacacgcgaggagaatcgtgagagaaagcggcttgcgcaactcga
agagctcgaatctagtgagacgacgggagtccgacggaccctcaggagtactcaggacaa
ctcggatccactggac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCAGAGTACAGTGCTGCGAGCCCA 690 587 2391 Antisense
ACACTCCGACAGGCAGCAGAAGCAT 218 109 2573 Antisense
GAAGCACTTCACCGAATGATCGCCG 309 341 2600 Antisense
GAAAGTCTCGAGAGACTCCTCCCGG 13 361 2624 Antisense
GACTAGTCCTTCCGATGGCAGAAAA 466 373 2667 Antisense
GAAAATCCAGAGACACTCCAACCGC 661 353 2687 Antisense
ACTCCAACCGCCAGTAGCAGTATGA 457 99 2701 Antisense
GAGAAAGCGGCTTGCGCAACTCGAA 254 393 2847 Antisense
TGAGACGACGGGAGTCCGACGGACC 702 411 2886 Antisense
ACGGACCCTCAGGAGTACTCAGGAC 380 95 2904 Antisense
TCAGGACAACTCGGATCCACTGGAC 90 623 2922 Antisense
Sequence Source Affymetrix Proprietary Database