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[185391_at]

Description H06I04.2 /REP_DB=WormBase Gene ID /WP=CE23794 /TR=Q9N5N8 /GB=AAF39867.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 09      4grp_total > 44      6grp_cgc > 07      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 167
1 2 3 4 range
A 201
(3)
P 		163.9
(-0.1)
P 		170.1
(0.4)
P 		128.3
(-3)
A 		73
B 233.3
(0.6)
P 		295.7
(3)
P 		208.2
(-0.4)
P 		142.8
(-3)
A 		153
C 225
(3)
P 		222.6
(2.8)
P 		167.1
(-3)
P 		223
(2.8)
A 		58
avr 219.8
(2.3) 		227.4
(3) 		181.8
(-1.4) 		164.7
(-3) 		63

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 112
1 2 3 4 5 6 range
A 225.7
(3)
A 		160.6
(-1.9)
P 		148.6
(-2.7)
A 		145.2
(-3)
P 		189.5
(0.3)
A 		200.7
(1.1)
A 		81
B 155
(1.7)
A 		113.6
(-3)
A 		165.8
(2.9)
M 		144
(0.4)
A 		166.9
(3)
P 		163.2
(2.6)
P 		53
C 154.2
(1.1)
M 		130.2
(-2.7)
A 		158.9
(1.9)
P 		165.9
(3)
A 		160.6
(2.1)
P 		128.5
(-3)
P 		37
avr 178.3
(3) 		134.8
(-3) 		157.8
(0.2) 		151.7
(-0.7) 		172.3
(2.2) 		164.1
(1) 		44
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 56
1 2 3 4 range
A 73.2
(3)
P 		35.6
(-1.1)
P 		44.5
(-0.2)
P 		18.8
(-3)
A 		54
B 74.4
(3)
P 		73
(2.8)
P 		48.2
(-0.6)
P 		30.4
(-3)
A 		44
C 74.5
(3)
P 		64
(1.3)
P 		58.2
(0.3)
P 		37.6
(-3)
A 		37
avr 74
(3) 		57.5
(0.8) 		50.3
(-0.2) 		28.9
(-3) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 35
1 2 3 4 5 6 range
A 41.6
(-0.8)
A 		57.1
(3)
P 		32.4
(-3)
A 		36.8
(-1.9)
P 		57.2
(3)
A 		48.9
(1)
A 		25
B 53.9
(0.2)
A 		37.8
(-3)
A 		40.3
(-2.5)
M 		38.1
(-2.9)
A 		67.8
(3)
P 		60.8
(1.6)
P 		30
C 57.1
(3)
M 		39.7
(-1.4)
A 		40.4
(-1.2)
P 		33.4
(-3)
A 		52
(1.7)
P 		43
(-0.6)
P 		24
avr 50.9
(0.9) 		44.9
(-0.7) 		37.7
(-2.6) 		36.1
(-3) 		59
(3) 		50.9
(0.9) 		23
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185391_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6765
Sequence Type Exemplar sequence
Representative Public ID H06I04.2 NCBI
Target Description H06I04.2 /REP_DB=WormBase Gene ID /WP=CE23794 /TR=Q9N5N8 /GB=AAF39867.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065256(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065256 NCBI Caenorhabditis elegans SurFeit homolog family member (sft-1) (sft-1) mRNA, complete cds. 11/11 None
GENEFINDER00000025129 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:3088179:3100109:1 11/11 None
H06I04.2 ENSEMBL cdna:known chromosome:CEL140:III:3086692:3091646:1 gene:H06I04.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3086691-3091638(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol sft-1
Ensembl H06I04.2
Entrez Gene 175415 Entrez gene
SwissProt Q9N5N8 EMBL-EBI
WormBase CE23794 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:258164_AT surfeit 1 (SURF1) at
CANINE:1586859_AT similar to Surfeit locus protein 1 cfa
DROSOPHILA_2:1625163_AT Surfeit 1 dm
DROSGENOME1:148292_AT Surfeit 1 dm
CHICKEN:GGA.11482.1.S1_AT surfeit 1 gga
CHICKEN:GGA.20013.1.S1_S_AT surfeit 1 gga
HG-U133_PLUS_2:204295_AT surfeit 1 hs
HG-U133A:204295_AT surfeit 1 hs
HG-FOCUS:204295_AT surfeit 1 hs
HG-U133A_2:204295_AT surfeit 1 hs
HUGENEFL:Z35093_AT surfeit 1 hs
U133_X3P:G4507318_3P_AT surfeit 1 hs
U133_X3P:HS.257150.0.A1_3P_AT surfeit 1 hs
HG-U133A_2:217646_AT surfeit 1 hs
HG-U133_PLUS_2:217646_AT surfeit 1 hs
HG-U133A:217646_AT surfeit 1 hs
HG-U95AV2:35646_AT surfeit 1 hs
MOE430A:1450561_A_AT surfeit gene 1 mm
MOUSE430_2:1450561_A_AT surfeit gene 1 mm
MOUSE430A_2:1450561_A_AT surfeit gene 1 mm
MU11KSUBA:M14689_CDS1_S_AT surfeit gene 1 mm
MU11KSUBB:MSA.1192.0_S_AT surfeit gene 1 mm
MG-U74AV2:101031_AT surfeit gene 1 mm
MU19KSUBA:TC14908_AT Surfeit gene 1, mRNA (cDNA clone MGC:6703 IMAGE:3584373) mm
MU19KSUBC:TC36255_AT Surfeit gene 1, mRNA (cDNA clone MGC:6703 IMAGE:3584373) mm
MU19KSUBC:TC36255_G_AT Surfeit gene 1, mRNA (cDNA clone MGC:6703 IMAGE:3584373) mm
RG-U34C:RC_AI104283_AT surfeit 1 rn
RAE230A:1398857_AT surfeit 1 rn
RAT230_2:1398857_AT surfeit 1 rn
YEAST_2:1773521_AT Mitochondrial inner membrane protein required for normal respiration, possible chaperone involved in assembly of cytochrome c oxidase; similar to SURF1 from mammals, chickens, and D. melanogaster Sc
Protein Similarities
Method ID Description E-Value
blast AAM48540 Hypothetical protein H06I04.1b [Caenorhabditis elegans] ref|NP_741104.1| H06I04.1b [Caenorhabditis elegans] 0.0
blast AAF39866 Hypothetical protein H06I04.1a [Caenorhabditis elegans] ref|NP_741103.1| H06I04.1a [Caenorhabditis elegans] 0.0
blast AAF39867 Surfeit homolog protein 1 [Caenorhabditis elegans] ref|NP_497657.1| SurFeit homolog family member (sft-1) [Caenorhabditis elegans] 1.0E-176
blast AAF39867 Surfeit homolog protein 1 [Caenorhabditis elegans] ref|NP_497657.1| SurFeit homolog family member (sft-1) [Caenorhabditis elegans] 1.0E-145
blast CAE74667 Hypothetical protein CBG22468 [Caenorhabditis briggsae] 1.0E-121
blast NP_003163 surfeit 1 [Homo sapiens] emb|CAI12837.1| surfeit 1 [Homo sapiens] gb|AAH28314.1| Surfeit 1 [Homo sapiens] sp|Q15526|SURF1_HUMAN Surfeit locus protein 1 gb|AAH71658.1| Surfeit 1 [Homo sapiens] emb|CAA84476.1| SURF-1 [Homo sapiens] 8.0E-50
Protein Domains
Database ID Description E-Value
Pfam IPR002994 EMBL-EBI Surf1 protein 3.3E-50
Pfam IPR002994 EMBL-EBI Surf1 protein 1.5E-54
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497657.1 2 81-103,302-319
H06I04.2 2 81-103,302-319
GENEFINDER00000025129 1 56-78
Sequence
Target Sequence 
>C. ELEGANS:185391_AT
aagggcgactgaatcaaactgcacaggaattacctgaagatttgagttgtgaatccctcg
aacccctcgaatactgtagggttactgtaaccggcgagtttctccacgaaaaggagttca
tcatcagtccccgaggtcgtttcgaccccggaaagaagaccagcgccgccgccggctcaa
tgttgtctgaaaatgaaatgagcagtcatggaggacacctaatcactccgtttagactga
aaaattcgggaaaaataatcctaatcaaccgtggatggcttccaagcttctactttgacc
cggaaacccgccaaaaaaccaatccacgtggcacccttaccctgccggcaatcgtccgaa
aaaccgagaaacggccccaattcgtaggccaaaatgtgccggaacaaggtgtttggtact
atcgggacctgaatcaaatggctaaacattatggaacagagccagtgttgttagatgcag
cttatgaaactacagtgccgggtggtccaattggtggtcaaaccaatatcaacgttcgga
atgagcatttgaactatttgacgactt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGGGCGACTGAATCAAACTGCACA 640 159 362 Antisense
TTTGAGTTGTGAATCCCTCGAACCC 231 667 402 Antisense
CCTCGAACCCCTCGAATACTGTAGG 281 265 417 Antisense
TTACTGTAACCGGCGAGTTTCTCCA 370 661 443 Antisense
AAAAGGAGTTCATCATCAGTCCCCG 603 131 470 Antisense
CGCCGGCTCAATGTTGTCTGAAAAT 546 257 531 Antisense
GAGCAGTCATGGAGGACACCTAATC 348 387 561 Antisense
GACACCTAATCACTCCGTTTAGACT 532 367 575 Antisense
AACCGTGGATGGCTTCCAAGCTTCT 175 147 628 Antisense
CTGCCGGCAATCGTCCGAAAAACCG 217 233 703 Antisense
GAGCATTTGAACTATTTGACGACTT 205 389 904 Antisense
Sequence Source Affymetrix Proprietary Database