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[185371_at]

Description F38H12.5 /REP_DB=WormBase Gene ID /WP=CE10084 /TR=O16345 /GB=AAC25897.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 78      4grp_total > 04      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 69
1 2 3 4 range
A 20.1
(-2.1)
A 		13.8
(-2.7)
A 		10.6
(-3)
A 		72.9
(3)
A 		62
B 20.4
(-2.4)
A 		16.6
(-3)
P 		18.7
(-2.7)
A 		53.3
(3)
A 		37
C 45.3
(-0.3)
A 		23.1
(-2.4)
P 		17.2
(-3)
A 		79.2
(3)
P 		62
avr 28.6
(-1.5) 		17.8
(-2.7) 		15.5
(-3) 		68.5
(3) 		53

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 41
1 2 3 4 5 6 range
A 26.3
(0.6)
A 		1.7
(-3)
A 		12.7
(-1.4)
A 		13.6
(-1.3)
A 		18.9
(-0.5)
P 		43.1
(3)
P 		41
B 21.6
(-3)
A 		28.1
(-0.2)
P 		24
(-2)
P 		26.7
(-0.8)
A 		35.6
(3)
P 		33.6
(2.1)
P 		14
C 6.7
(-3)
A 		21.9
(3)
A 		16.6
(0.9)
A 		15.1
(0.3)
A 		12.9
(-0.6)
A 		19.9
(2.2)
A 		15
avr 18.2
(-2.6) 		17.2
(-3) 		17.8
(-2.8) 		18.5
(-2.5) 		22.5
(-0.9) 		32.2
(3) 		15
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 7.3
(0.4)
A 		3
(-2.8)
A 		2.8
(-3)
A 		10.7
(3)
A 		8
B 6.5
(-1)
A 		4.1
(-3)
P 		4.3
(-2.8)
A 		11.4
(3)
A 		7
C 15
(3)
A 		6.6
(-2.6)
P 		6
(-3)
A 		13.4
(1.9)
P 		9
avr 9.6
(1.2) 		4.6
(-2.8) 		4.4
(-3) 		11.8
(3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 4.8
(-0.5)
A 		0.6
(-3)
A 		2.8
(-1.7)
A 		3.5
(-1.2)
A 		5.7
(0.1)
P 		10.5
(3)
P 		10
B 7.5
(-1.8)
A 		9.3
(-0.6)
P 		5.8
(-3)
P 		7.1
(-2.1)
A 		14.5
(3)
P 		12.5
(1.6)
P 		9
C 2.5
(-3)
A 		6.7
(3)
A 		4.2
(-0.6)
A 		3
(-2.3)
A 		4.2
(-0.6)
A 		6.7
(3)
A 		4
avr 4.9
(-2.4) 		5.5
(-1.7) 		4.3
(-3) 		4.5
(-2.8) 		8.1
(1.1) 		9.9
(3) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185371_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15113
Sequence Type Exemplar sequence
Representative Public ID F38H12.5 NCBI
Target Description F38H12.5 /REP_DB=WormBase Gene ID /WP=CE10084 /TR=O16345 /GB=AAC25897.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071737(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071737 NCBI Caenorhabditis elegans F38H12.5 (F38H12.5) mRNA, complete cds. 9/11 None
GENEFINDER00000014197 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:4081045:4082865:-1 9/11 None
F38H12.5 ENSEMBL cdna:known chromosome:CEL140:V:4081273:4082665:-1 gene:F38H12.5 9/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:4081272-4083023(-) 86.79 100.0
Public Domain and Genome References
Gene Title
Ensembl F38H12.5
Entrez Gene 185484 Entrez gene
WormBase CE39099 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC25897 Hypothetical protein F38H12.5 [Caenorhabditis elegans] 1.0E-132
blast NP_504138 F38H12.5 [Caenorhabditis elegans] 1.0E-74
blast AAC25897 Hypothetical protein F38H12.5 [Caenorhabditis elegans] 3.0E-52
blast NP_504138 F38H12.5 [Caenorhabditis elegans] 2.0E-51
blast CAE58196 Hypothetical protein CBG01290 [Caenorhabditis briggsae] 3.0E-50
blast CAE58200 Hypothetical protein CBG01294 [Caenorhabditis briggsae] 2.0E-17
Protein Domains
Database ID Description E-Value
Pfam IPR002892 EMBL-EBI Protein of unknown function DUF40 1.1E-25
Pfam IPR002892 EMBL-EBI Protein of unknown function DUF40 1.1E-59
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504138.2 2 32-54,94-113
F38H12.5 2 32-54,94-113
GENEFINDER00000014197 6 5-27,47-69,84-106,119-141,181-203,215-237
Sequence
Target Sequence 
>C. ELEGANS:185371_AT
ggcctaggcaaactattccatttcaatttatgaaggaagccggaaatttagcacaaacac
tacaaaacagaaatcaaggaaaaacatgtatttttctggccacctatttccctctttact
ttttcaattatcgaagttacatcccaaacatgttcattgttgctctcgttttcatgtatc
ccttaatagatggctcaatgttgtttatttactgcgaaaatgtcatacaaatttctccag
gatgcaaaaatgcagcttgtgttttgtcggaatgctattcactttattggctagcctatg
aaaaagtagtgtatacttcaattatcgcattatcattgttgctgtctttga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCCTAGGCAAACTATTCCATTTCA 465 535 29 Antisense
CATGTATTTTTCTGGCCACCTATTT 699 209 113 Antisense
GAAGTTACATCCCAAACATGTTCAT 79 337 161 Antisense
ACATGTTCATTGTTGCTCTCGTTTT 497 107 176 Antisense
CTCGTTTTCATGTATCCCTTAATAG 681 229 193 Antisense
GGCTCAATGTTGTTTATTTACTGCG 385 537 220 Antisense
GTCATACAAATTTCTCCAGGATGCA 556 467 250 Antisense
GCTTGTGTTTTGTCGGAATGCTATT 214 307 283 Antisense
TATTCACTTTATTGGCTAGCCTATG 654 663 304 Antisense
GTATACTTCAATTATCGCATTATCA 98 455 339 Antisense
GCATTATCATTGTTGCTGTCTTTGA 635 307 355 Antisense
Sequence Source Affymetrix Proprietary Database