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[185363_at]

Description Y50D7A.3 /REP_DB=WormBase Gene ID /WP=CE26140 /TR=Q9N3L4 /GB=AAF59648.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 54      4grp_total > 54      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 72
1 2 3 4 range
A 66.2
(1.5)
A 		64.9
(1.4)
A 		83.3
(3)
A 		14.7
(-3)
A 		69
B 68.5
(3)
A 		58.9
(2)
A 		11.4
(-3)
A 		44.5
(0.5)
A 		57
C 43.7
(2.3)
A 		18.9
(-3)
A 		27.5
(-1.2)
A 		46.8
(3)
A 		28
avr 59.5
(3) 		47.6
(0) 		40.7
(-1.7) 		35.3
(-3) 		24

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 61
1 2 3 4 5 6 range
A 14.8
(-1.2)
A 		5.2
(-3)
A 		36.9
(3)
A 		12.6
(-1.6)
A 		11.3
(-1.8)
A 		32.6
(2.2)
A 		32
B 10.3
(-2.3)
A 		59.7
(3)
A 		46.3
(1.6)
A 		25.5
(-0.7)
A 		3.6
(-3)
A 		40.3
(0.9)
A 		56
C 9.4
(-2.6)
A 		51.6
(1.7)
A 		64.4
(3)
A 		16.7
(-1.9)
A 		5.5
(-3)
A 		10.5
(-2.5)
A 		59
avr 11.5
(-2.3) 		38.8
(1.5) 		49.2
(3) 		18.3
(-1.4) 		6.8
(-3) 		27.8
(-0) 		42
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 22
1 2 3 4 range
A 24.1
(3)
A 		14.1
(0.3)
A 		21.8
(2.4)
A 		2.2
(-3)
A 		22
B 21.8
(3)
A 		14.5
(0.7)
A 		2.7
(-3)
A 		9.5
(-0.9)
A 		19
C 14.5
(3)
A 		5.4
(-3)
A 		9.6
(-0.2)
A 		7.9
(-1.4)
A 		9
avr 20.1
(3) 		11.3
(-0.9) 		11.4
(-0.8) 		6.5
(-3) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 18
1 2 3 4 5 6 range
A 2.7
(-2.2)
A 		1.9
(-3)
A 		8
(3)
A 		3.2
(-1.7)
A 		3.4
(-1.5)
A 		7.9
(2.9)
A 		6
B 3.6
(-2.3)
A 		19.9
(3)
A 		11.3
(0.2)
A 		6.7
(-1.3)
A 		1.5
(-3)
A 		15
(1.4)
A 		18
C 3.5
(-2.3)
A 		15.7
(2.7)
A 		16.4
(3)
A 		3.4
(-2.3)
A 		1.8
(-3)
A 		3.5
(-2.3)
A 		15
avr 3.3
(-2.4) 		12.5
(3) 		11.9
(2.7) 		4.4
(-1.7) 		2.2
(-3) 		8.8
(0.8) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185363_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8187
Sequence Type Exemplar sequence
Representative Public ID Y50D7A.3 NCBI
Target Description Y50D7A.3 /REP_DB=WormBase Gene ID /WP=CE26140 /TR=Q9N3L4 /GB=AAF59648.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064780(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064780 NCBI Caenorhabditis elegans Y50D7A.3a (Y50D7A.3) mRNA, complete cds. 11/11 None
Y50D7A.3a ENSEMBL cdna:known chromosome:CEL140:III:254534:264395:1 gene:Y50D7A.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000021187 5/11 Cross Hyb Matching Probes None
GENEFINDER00000021202 8/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
190101 2 1 NM_064780 185363_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:257512-264395(+) 87.27 100.0
Public Domain and Genome References
Ensembl Y50D7A.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF59648 Hypothetical protein Y50D7A.3a [Caenorhabditis elegans] ref|NP_497181.2| Y50D7A.3a [Caenorhabditis elegans] 0.0
blast CAE69504 Hypothetical protein CBG15712 [Caenorhabditis briggsae] 1.0E-142
blast NP_727548 CG1830-PB, isoform B [Drosophila melanogaster] gb|AAN09640.1| CG1830-PB, isoform B [Drosophila melanogaster] gb|AAK93059.1| GH28523p [Drosophila melanogaster] 5.0E-96
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 6.0E-61
hanks 2.1.19 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; PhKgM 7.0E-79
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.3E-82
Sequence
Target Sequence 
>C. ELEGANS:185363_AT
ggttcaaaacggcaatcatctgggttcgattcttccaacgtctggccaaatacaaatatc
ttaaaactgtcattgatcgggatgtacttaggaagagaccatttagagatcgagatcgga
aaaatcatcgaaatttcagttattcgacggaaaattcacaatattcagacgaaattttgc
aaaaaatttcggcgaaaattgatctgaaattgctggaaatgattcgtcacgaagcggaat
catcaatgttcagtgtatatggtcactgggtgaatcgagggttctactattccagagata
tgctattcgccaacaagccacgcccgaaaattgccaagaaaactgacggagcagctgctg
tggagcagaagcttaaagtcccgccgatagctttgagaaaattcaattttctggctgaaa
ttttccaaaaaaaaaaattggattattttgactggaaaatgttcaaaattccggatttta
actcaaattcagtcaaaaattcgcgattttctcttcgcttttatgtaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTTCAAAACGGCAATCATCTGGGT 199 507 653 Antisense
TCATCTGGGTTCGATTCTTCCAACG 277 621 668 Antisense
GATTCTTCCAACGTCTGGCCAAATA 33 431 680 Antisense
GGTCACTGGGTGAATCGAGGGTTCT 604 501 913 Antisense
CGAGGGTTCTACTATTCCAGAGATA 365 247 928 Antisense
GAGATATGCTATTCGCCAACAAGCC 393 397 947 Antisense
GCCACGCCCGAAAATTGCCAAGAAA 515 277 969 Antisense
GAGCAGAAGCTTAAAGTCCCGCCGA 250 389 1015 Antisense
AGTCCCGCCGATAGCTTTGAGAAAA 226 61 1029 Antisense
AAAATTCGCGATTTTCTCTTCGCTT 38 135 1148 Antisense
GATTTTCTCTTCGCTTTTATGTAAA 614 427 1157 Antisense
Sequence Source Affymetrix Proprietary Database