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[185357_at]

Description Y37D8A.16 /REP_DB=WormBase Gene ID /WP=CE20220 /TR=Q9XWV6 /GB=CAA21534.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 10      6grp_high1_10_10 > 01      4grp_highest_8_8 > 62      4grp_high_8_8 > 18      6grp_highest_nonorm > 57      6grp_total > 04      4grp_total > 30      6grp_high1_sub > De      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 630
1 2 3 4 range
A 1964.4
(0.3)
P 		1926
(-0.1)
P 		1674.6
(-3)
P 		2199.6
(3)
P 		525
B 2295.5
(3)
P 		1760.2
(-2.2)
P 		1681.8
(-3)
P 		1984.5
(-0)
P 		614
C 2197.4
(3)
P 		2002.9
(0.8)
P 		1665.1
(-3)
P 		1807.9
(-1.4)
P 		532
avr 2152.4
(3) 		1896.4
(-0.2) 		1673.8
(-3) 		1997.3
(1.1) 		479

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1712
1 2 3 4 5 6 range
A 3382.4
(3)
P 		2368.9
(-0.6)
P 		2437.8
(-0.3)
P 		2454.7
(-0.3)
P 		2274.9
(-0.9)
P 		1670.9
(-3)
P 		1712
B 2873
(3)
P 		2747
(2.2)
P 		2643.5
(1.6)
P 		2394.1
(0.1)
P 		2431.4
(0.3)
P 		1874.2
(-3)
P 		999
C 2740.4
(2.7)
P 		2601.6
(1.9)
P 		1921.6
(-2.3)
P 		2787
(3)
P 		1812.9
(-3)
P 		2091
(-1.3)
P 		974
avr 2998.6
(3) 		2572.5
(0.7) 		2334.3
(-0.6) 		2545.3
(0.6) 		2173.1
(-1.4) 		1878.7
(-3) 		1120
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 427
1 2 3 4 range
A 715.2
(3)
P 		418.1
(-1.5)
P 		437.8
(-1.2)
P 		322
(-3)
P 		393
B 732
(3)
P 		434.7
(-2.2)
P 		389.7
(-3)
P 		422.9
(-2.4)
P 		342
C 727.4
(3)
P 		575.9
(0.8)
P 		580.3
(0.9)
P 		304.8
(-3)
P 		423
avr 724.9
(3) 		476.2
(-1) 		469.3
(-1.1) 		349.9
(-3) 		375

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 609
1 2 3 4 5 6 range
A 623.5
(-0)
P 		842.5
(3)
P 		530.9
(-1.3)
P 		622.6
(-0)
P 		686.5
(0.9)
P 		406.8
(-3)
P 		436
B 999.7
(3)
P 		914.4
(1.6)
P 		641.9
(-2.9)
P 		634.1
(-3)
P 		987.8
(2.8)
P 		698.9
(-1.9)
P 		366
C 1015.8
(3)
P 		793.8
(0.5)
P 		489.1
(-3)
P 		560.5
(-2.2)
P 		586.6
(-1.9)
P 		700
(-0.6)
P 		527
avr 879.7
(3) 		850.2
(2.5) 		554
(-3) 		605.7
(-2) 		753.6
(0.7) 		601.9
(-2.1) 		326
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185357_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7218
Sequence Type Exemplar sequence
Representative Public ID Y37D8A.16 NCBI
Target Description Y37D8A.16 /REP_DB=WormBase Gene ID /WP=CE20220 /TR=Q9XWV6 /GB=CAA21534.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067282(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067282 NCBI Caenorhabditis elegans Y37D8A.16 (Y37D8A.16) mRNA, complete cds. 11/11 None
SNAP00000028784 ENSEMBL cdna:SNAP chromosome:CEL140:III:12915170:12925498:-1 10/11 None
GENEFINDER00000028816 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:12915170:12918543:-1 11/11 None
Y37D8A.16 ENSEMBL cdna:known chromosome:CEL140:III:12915038:12918548:-1 gene:Y37D8A.16 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:12915165-12918539(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y37D8A.16
Entrez Gene 176709 Entrez gene
SwissProt Q9XWV6 EMBL-EBI
WormBase CE20220 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA21541 Hypothetical protein Y37D8A.17 [Caenorhabditis elegans] ref|NP_499684.1| Y37D8A.17 [Caenorhabditis elegans] sp|Q9XWV0|TMM33_CAEEL Hypothetical UPF0121 protein Y37D8A.17 in chromosome I 1.0E-126
blast CAE69548 Hypothetical protein CBG15760 [Caenorhabditis briggsae] 1.0E-115
blast CAA21534 Hypothetical protein Y37D8A.16 [Caenorhabditis elegans] ref|NP_499683.1| Y37D8A.16 [Caenorhabditis elegans] 3.0E-47
blast CAA21534 Hypothetical protein Y37D8A.16 [Caenorhabditis elegans] ref|NP_499683.1| Y37D8A.16 [Caenorhabditis elegans] 4.0E-45
blast CAE69549 Hypothetical protein CBG15761 [Caenorhabditis briggsae] 6.0E-41
Protein Domains
Database ID Description E-Value
Pfam IPR005344 EMBL-EBI Protein of unknown function UPF0121 1.6E-90
Pfam IPR005344 EMBL-EBI Protein of unknown function UPF0121 5.9E-85
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499683.1 4 15-37,44-66,81-103,116-138
Y37D8A.16 4 15-37,44-66,81-103,116-138
SNAP00000028784 7 51-73,125-147,199-221,286-308,315-337,352-374,386-408
GENEFINDER00000028816 4 15-37,44-66,81-103,116-138
Sequence
Target Sequence 
>C. ELEGANS:185357_AT
ccagctgctcatcggtatgggcattaaaattgtgattattattctcacaattgtcacact
gatcctcctggacccagcatatgtcaccgcatacatctcaattaattatgacattgtcat
catttatattgtctctgcactgacacttctctactgccttgtctcaattctcatgtcatt
ccttttggcaaaacgtggagaggatactccgctaacgaattgtggatttgcggaaatcat
cttctcaactgctggaattataggatggcttatcattattggaattggtggcacaatctc
tcagagaacaattattgaaactggcgaacgatttggatggattggggcaatggctggact
caacgttggatgcttcctcggcatcgctgccattttcgttgcaaatcttgtcaacgataa
gattctacagcgctcgcaaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCAGCTGCTCATCGGTATGGGCATT 315 273 27 Antisense
TCACAATTGTCACACTGATCCTCCT 34 629 71 Antisense
TCCTCCTGGACCCAGCATATGTCAC 324 593 89 Antisense
GTCACCGCATACATCTCAATTAATT 70 467 109 Antisense
TATTGTCTCTGCACTGACACTTCTC 644 665 153 Antisense
TGCCTTGTCTCAATTCTCATGTCAT 319 585 181 Antisense
TTCTCATGTCATTCCTTTTGGCAAA 678 691 194 Antisense
GAGGATACTCCGCTAACGAATTGTG 595 403 226 Antisense
GGAAATCATCTTCTCAACTGCTGGA 381 527 258 Antisense
GGACTCAACGTTGGATGCTTCCTCG 282 525 382 Antisense
ATAAGATTCTACAGCGCTCGCAAAA 480 25 443 Antisense
Sequence Source Affymetrix Proprietary Database