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[185350_at]

Description F54D11.1 /REP_DB=WormBase Gene ID /WP=CE11068 /TR=Q22993 /GB=AAB04824.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 23      6grp_high1_10_10 > 21      4grp_highest_8_8 > 17      4grp_high_8_8 > 60      6grp_highest_nonorm > 16      6grp_total > 22      4grp_total > 59      6grp_highest_sub > 01_02_03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9064
1 2 3 4 range
A 8822.2
(1.1)
P 		10085
(1.9)
P 		11579.1
(3)
P 		3061.1
(-3)
P 		8518
B 9524.5
(1.2)
P 		9928.8
(1.5)
P 		12125.1
(3)
P 		3261.3
(-3)
P 		8864
C 8330.9
(0.8)
P 		9408.9
(1.5)
P 		11451.3
(3)
P 		3080
(-3)
P 		8371
avr 8892.5
(1) 		9807.6
(1.7) 		11718.5
(3) 		3134.1
(-3) 		8584

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6748
1 2 3 4 5 6 range
A 9533.2
(2.5)
P 		8482.2
(1.6)
P 		10052.6
(3)
P 		7145.5
(0.4)
P 		4630.1
(-1.8)
P 		3304.3
(-3)
P 		6748
B 9088.1
(2.5)
P 		9518
(3)
P 		9484
(3)
P 		7636.2
(0.9)
P 		4850.2
(-2.2)
P 		4138.6
(-3)
P 		5379
C 8313.8
(1.9)
P 		9281.1
(3)
P 		8871.7
(2.6)
P 		6037.2
(-0.5)
P 		4835.7
(-1.9)
P 		3795.1
(-3)
P 		5486
avr 8978.4
(2.5) 		9093.8
(2.6) 		9469.4
(3) 		6939.6
(0.3) 		4772
(-1.9) 		3746
(-3) 		5723
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3543
1 2 3 4 range
A 3212.2
(3)
P 		2189.2
(0.8)
P 		3027
(2.6)
P 		448.1
(-3)
P 		2764
B 3037.3
(3)
P 		2452.2
(1.5)
P 		2809.7
(2.4)
P 		695
(-3)
P 		2342
C 2757.8
(0.9)
P 		2705.1
(0.8)
P 		3991.1
(3)
P 		519.3
(-3)
P 		3472
avr 3002.4
(2.4) 		2448.8
(1.2) 		3275.9
(3) 		554.1
(-3) 		2722

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2364
1 2 3 4 5 6 range
A 1757.4
(-0.4)
P 		3016.7
(3)
P 		2189.3
(0.8)
P 		1812.3
(-0.3)
P 		1397.2
(-1.4)
P 		804.5
(-3)
P 		2212
B 3162.2
(3)
P 		3168.1
(3)
P 		2303
(-0.2)
P 		2022.6
(-1.2)
P 		1970.6
(-1.4)
P 		1543.3
(-3)
P 		1625
C 3081.5
(3)
P 		2832
(2.2)
P 		2258
(0.4)
P 		1214.1
(-3)
P 		1564.8
(-1.9)
P 		1270.4
(-2.8)
P 		1867
avr 2667
(1.9) 		3005.6
(3) 		2250.1
(0.5) 		1683
(-1.4) 		1644.2
(-1.5) 		1206.1
(-3) 		1800
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185350_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15112
Sequence Type Exemplar sequence
Representative Public ID F54D11.1 NCBI
Target Description F54D11.1 /REP_DB=WormBase Gene ID /WP=CE11068 /TR=Q22993 /GB=AAB04824.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071847(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071847 NCBI Caenorhabditis elegans F54D11.1 (F54D11.1) mRNA, complete cds. 11/11 None
GENEFINDER00000035035 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:4618690:4635005:1 11/11 None
F54D11.1 ENSEMBL cdna:known chromosome:CEL140:V:4628510:4635080:1 gene:F54D11.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000035032 5/11 Cross Hyb Matching Probes None
SNAP00000035033 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:4628581-4635005(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F54D11.1
Entrez Gene 178854 Entrez gene
SwissProt Q22993 EMBL-EBI
WormBase CE11068 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:258218_AT phosphoethanolamine N-methyltransferase 1 / PEAMT 1 (NMT1) at
ATH1-121501:258217_AT phosphoethanolamine N-methyltransferase 1 / PEAMT 1 (NMT1) at
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
8168 methyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB04824 Hypothetical protein F54D11.1 [Caenorhabditis elegans] ref|NP_504248.1| F54D11.1 [Caenorhabditis elegans] 0.0
blast CAE64156 Hypothetical protein CBG08775 [Caenorhabditis briggsae] 0.0
blast AAB04823 Hypothetical protein F54D11.2 [Caenorhabditis elegans] ref|NP_504247.2| F54D11.2 [Caenorhabditis elegans] 0.0
blast CAE64155 Hypothetical protein CBG08774 [Caenorhabditis briggsae] 0.0
blast NP_917067 putative phosphoethanolamine methyltransferase [Oryza sativa (japonica cultivar-group)] 8.0E-54
Sequence
Target Sequence 
>C. ELEGANS:185350_AT
gtggtggagctagacaggttgccgatgagtttggagttcatgttcatggaattgatttgt
cttcaaatatgttggcaatcgctttggaacgacttcacgaggaaaaggattcacgtgtca
agtactcgattactgatgcattggtctatcaattcgaggataattcattcgattacgtgt
tctcgagagattgtattcaacacattccagacaccgagaagcttttctcaagaatctata
aagcactgaaaccaggaggcaaggtgctcatcacaatgtatggaaagggttacggagagc
aaagtgacaagttcaagacctatgttgctcagagagcatatttcttgaaaaacttgaagg
agatcgctgatatcgccaacaagacaggttttgtcaatgttcagactgagaatatgaccc
caagattcaaggagatccttcttgaagaacgtggtcatttggagcagaatgaggcagagt
tcatgtcgaagtttacccaacgcgaaagagattccctcatctctggatggaccgac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGGTGGAGCTAGACAGGTTGCCGA 471 483 719 Antisense
GGCAATCGCTTTGGAACGACTTCAC 21 535 792 Antisense
AGGATTCACGTGTCAAGTACTCGAT 467 57 824 Antisense
GTACTCGATTACTGATGCATTGGTC 423 459 840 Antisense
GTATTCAACACATTCCAGACACCGA 464 453 911 Antisense
GGAGGCAAGGTGCTCATCACAATGT 211 515 973 Antisense
GTTCAAGACCTATGTTGCTCAGAGA 408 445 1029 Antisense
GAAGGAGATCGCTGATATCGCCAAC 106 335 1074 Antisense
GTTCATGTCGAAGTTTACCCAACGC 430 441 1197 Antisense
ACCCAACGCGAAAGAGATTCCCTCA 361 87 1213 Antisense
TTCCCTCATCTCTGGATGGACCGAC 689 697 1230 Antisense
Sequence Source Affymetrix Proprietary Database