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[185343_at]

Description T28C12.6 /REP_DB=WormBase Gene ID /WP=CE14349 /TR=O16699 /GB=AAB66161.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 19      6grp_high1_10_10 > 92      6grp_highest_nonorm > 81      6grp_total > 94      4grp_total > 49      6grp_highest_sub > 05      6grp_high1_sub > 04_05      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 321
1 2 3 4 range
A 75.3
(-3)
A 		375.3
(3)
P 		254.1
(0.6)
P 		101
(-2.5)
P 		300
B 54.6
(-3)
A 		374.9
(3)
P 		309.2
(1.8)
P 		80
(-2.5)
A 		320
C 76.3
(-3)
M 		322.9
(2.4)
P 		350
(3)
P 		85.6
(-2.8)
A 		274
avr 68.7
(-3) 		357.7
(3) 		304.4
(1.9) 		88.9
(-2.6) 		289

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 415
1 2 3 4 5 6 range
A 140.7
(-2.6)
P 		170.8
(-2.1)
P 		113.3
(-3)
P 		305.5
(-0.1)
P 		504.9
(3)
P 		259.6
(-0.8)
P 		392
B 131.4
(-3)
P 		199.4
(-1.6)
P 		159.6
(-2.4)
P 		423.9
(3)
P 		417.7
(2.9)
P 		285.5
(0.2)
P 		293
C 89.8
(-3)
P 		313.6
(1.6)
P 		131.2
(-2.1)
P 		371.5
(2.8)
P 		379.8
(3)
P 		226.1
(-0.2)
P 		290
avr 120.6
(-3) 		227.9
(-0.9) 		134.7
(-2.7) 		367
(1.7) 		434.1
(3) 		257.1
(-0.4) 		314
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 108
1 2 3 4 range
A 27.4
(-1.9)
A 		81.5
(3)
P 		66.4
(1.6)
P 		14.8
(-3)
P 		67
B 17.4
(-3)
A 		92.6
(3)
P 		71.7
(1.3)
P 		17.1
(-3)
A 		76
C 25.3
(-2.4)
M 		92.8
(1.4)
P 		122
(3)
P 		14.4
(-3)
A 		108
avr 23.4
(-2.3) 		89
(3) 		86.7
(2.8) 		15.4
(-3) 		74

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 145
1 2 3 4 5 6 range
A 25.9
(-2.9)
P 		60.8
(-1.3)
P 		24.7
(-3)
P 		77.5
(-0.5)
P 		152.3
(3)
P 		63.2
(-1.2)
P 		128
B 45.7
(-2.7)
P 		66.4
(-1.7)
P 		38.8
(-3)
P 		112.3
(0.4)
P 		169.7
(3)
P 		106.5
(0.1)
P 		131
C 33.3
(-3)
P 		95.7
(1.2)
P 		33.4
(-3)
P 		74.7
(-0.2)
P 		122.9
(3)
P 		75.7
(-0.2)
P 		90
avr 35
(-2.9) 		74.3
(-0.8) 		32.3
(-3) 		88.2
(-0.1) 		148.3
(3) 		81.8
(-0.4) 		116
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185343_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11723
Sequence Type Exemplar sequence
Representative Public ID T28C12.6 NCBI
Target Description T28C12.6 /REP_DB=WormBase Gene ID /WP=CE14349 /TR=O16699 /GB=AAB66161.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072217(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072217 NCBI Caenorhabditis elegans T28C12.6 (T28C12.6) mRNA, complete cds. 11/11 None
GENEFINDER00000017156 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:6336842:6338711:-1 11/11 None
T28C12.6 ENSEMBL cdna:known chromosome:CEL140:V:6337586:6339492:-1 gene:T28C12.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000017150 1/11 Cross Hyb Matching Probes None
SNAP00000017151 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6337585-6339492(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T28C12.6
Entrez Gene 189040 Entrez gene
SwissProt O16699 EMBL-EBI
WormBase CE14349 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB66161 Hypothetical protein T28C12.6 [Caenorhabditis elegans] ref|NP_504618.1| T28C12.6 [Caenorhabditis elegans] 0.0
blast AAB66161 Hypothetical protein T28C12.6 [Caenorhabditis elegans] ref|NP_504618.1| T28C12.6 [Caenorhabditis elegans] 1.0E-139
blast CAE71866 Hypothetical protein CBG18914 [Caenorhabditis briggsae] 1.0E-130
blast CAE71866 Hypothetical protein CBG18914 [Caenorhabditis briggsae] 1.0E-114
blast AAC78198 Hypothetical protein F54E2.2 [Caenorhabditis elegans] ref|NP_503760.1| F54E2.2 [Caenorhabditis elegans] 1.0E-13
blast AAC78198 Hypothetical protein F54E2.2 [Caenorhabditis elegans] ref|NP_503760.1| F54E2.2 [Caenorhabditis elegans] 2.0E-13
Sequence
Target Sequence 
>C. ELEGANS:185343_AT
ttggttttttcaatcctccatcttacgacacaccaaacaattttaggttcaacagttata
atcctagcagtaacaatgtgaacccacaatttaacgtttttcggtcatattccaatgatc
ttgttggatccctcccattatgtcgtaactatttccattcttgtatgacttccacatctt
gcgaatcaggaacgatatgcacaaatggattagaacaaggatcgtgttgcacgaatccta
gccaagctagctgcccatctgtaacatctatgaacatcaactgcagaaaattgagaagtg
tgaactggtgtaacaatgatttcgactgtcggggaggatctaccactgcttcaatgtgtt
gtccaactggctgtaattataacatgtgtgtacacgttggtgcacaagtatatcacccaa
gaagaagcgttgtcttcaaatcattatcgacagcagctacaacacctgatcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGGTTTTTTCAATCCTCCATCTTA 385 707 437 Antisense
AACGTTTTTCGGTCATATTCCAATG 507 143 529 Antisense
CCAATGATCTTGTTGGATCCCTCCC 208 277 548 Antisense
ATCCCTCCCATTATGTCGTAACTAT 250 37 564 Antisense
GTCGTAACTATTTCCATTCTTGTAT 669 471 578 Antisense
GTATGACTTCCACATCTTGCGAATC 695 453 599 Antisense
TGTTGCACGAATCCTAGCCAAGCTA 474 563 661 Antisense
TAGCTGCCCATCTGTAACATCTATG 366 651 684 Antisense
CGACTGTCGGGGAGGATCTACCACT 690 247 759 Antisense
TACCACTGCTTCAATGTGTTGTCCA 103 651 777 Antisense
GACAGCAGCTACAACACCTGATCAA 336 369 885 Antisense
Sequence Source Affymetrix Proprietary Database