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[185324_at]

Description F46F5.16 /REP_DB=WormBase Gene ID /WP=CE19432 /TR=Q9TXX1 /GB=AAC78193.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 93      4grp_total > 11      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 94
1 2 3 4 range
A 2.3
(-3)
A 		37.4
(-0.5)
A 		24.3
(-1.4)
A 		86.5
(3)
P 		84
B 25.2
(-1.1)
A 		8
(-3)
A 		10
(-2.8)
A 		61.7
(3)
P 		54
C 35.1
(-1.3)
A 		20.6
(-2.3)
A 		10.6
(-3)
A 		96.6
(3)
P 		86
avr 20.9
(-2.5) 		22
(-2.4) 		15
(-3) 		81.6
(3) 		67

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 47
1 2 3 4 5 6 range
A 25.4
(0)
A 		1.4
(-3)
A 		48.8
(3)
P 		24.5
(-0.1)
P 		41.9
(2.1)
P 		27.3
(0.3)
A 		47
B 33.2
(0.8)
A 		44.4
(2.7)
A 		20.7
(-1.4)
A 		46.4
(3)
A 		34.6
(1)
P 		11.1
(-3)
A 		35
C 2.9
(-3)
A 		37.9
(3)
A 		10.7
(-1.7)
A 		6.8
(-2.3)
A 		28.9
(1.5)
A 		29.1
(1.5)
A 		35
avr 20.5
(-3) 		27.9
(0) 		26.7
(-0.5) 		25.9
(-0.8) 		35.1
(3) 		22.5
(-2.2) 		15
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 0.8
(-3)
A 		8.1
(0.7)
A 		6.4
(-0.2)
A 		12.7
(3)
P 		12
B 8
(0.2)
A 		2
(-3)
A 		2.3
(-2.8)
A 		13.1
(3)
P 		11
C 11.6
(0.8)
A 		5.9
(-2)
A 		3.7
(-3)
A 		16.3
(3)
P 		13
avr 6.8
(-1.4) 		5.3
(-2.3) 		4.1
(-3) 		14
(3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 4.7
(-0.9)
A 		0.5
(-3)
A 		10.6
(2)
P 		6.2
(-0.2)
P 		12.6
(3)
P 		6.6
(0)
A 		12
B 11.6
(1.2)
A 		14.8
(3)
A 		5
(-2.5)
A 		12.3
(1.6)
A 		14.1
(2.6)
P 		4.1
(-3)
A 		11
C 1.1
(-3)
A 		11.5
(3)
A 		2.7
(-2.1)
A 		1.4
(-2.8)
A 		9.4
(1.8)
A 		9.8
(2)
A 		10
avr 5.8
(-3) 		8.9
(0) 		6.1
(-2.7) 		6.6
(-2.2) 		12
(3) 		6.8
(-2) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185324_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4707
Sequence Type Exemplar sequence
Representative Public ID F46F5.16 NCBI
Target Description F46F5.16 /REP_DB=WormBase Gene ID /WP=CE19432 /TR=Q9TXX1 /GB=AAC78193.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061408(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061408 NCBI Caenorhabditis elegans F46F5.16 (F46F5.16) mRNA, complete cds. 11/11 None
SNAP00000029511 ENSEMBL cdna:SNAP chromosome:CEL140:II:787318:788000:1 11/11 None
GENEFINDER00000029534 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:786739:789181:1 11/11 None
F46F5.16 ENSEMBL cdna:known chromosome:CEL140:II:787318:788000:1 gene:F46F5.16 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:787317-788000(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F46F5.16
Entrez Gene 185869 Entrez gene
SwissProt Q9TXX1 EMBL-EBI
WormBase CE19432 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC78194 Hypothetical protein F46F5.15 [Caenorhabditis elegans] ref|NP_493810.1| F46F5.15 [Caenorhabditis elegans] 9.0E-92
blast AAC78193 Hypothetical protein F46F5.16 [Caenorhabditis elegans] ref|NP_493809.1| F46F5.16 [Caenorhabditis elegans] 4.0E-87
blast AAC78193 Hypothetical protein F46F5.16 [Caenorhabditis elegans] ref|NP_493809.1| F46F5.16 [Caenorhabditis elegans] 1.0E-83
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493809.1 1 156-178
F46F5.16 1 156-178
SNAP00000029511 1 156-178
GENEFINDER00000029534 3 10-27,52-69,215-234
Sequence
Target Sequence 
>C. ELEGANS:185324_AT
gtgtgcgagccaactttatgtgaaatcaaagtggaatggataacgttggcaacgtacaac
aatagcaaaggaagaaaagcaataaaagaagcggtggttatcaagacagactatagacaa
ggcaataccacagtagctctgagcgctaaggatagagcatccgttatcgataccataaat
gagaaagtcgactcgaatgttgtgcatttgatcaatgcggaaattatccgttctaaggag
aaagtgaacaaaaaagttgtgagagcaatggtaagaacagctgtttctcaggctattgat
gcacctatcatgtttaccaatcctgcgaaaagcaatagaactctcaaacaagctatcatc
agttgcggtaaacacgcggtccggcttcaacaattcgtttttatggatcccaatgatctt
tcttcattctcatcgaaattcttgatcacaatgccttacatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGTGCGAGCCAACTTTATGTGAAA 634 487 61 Antisense
GAAGCGGTGGTTATCAAGACAGACT 602 341 148 Antisense
GCAATACCACAGTAGCTCTGAGCGC 293 323 182 Antisense
TAGAGCATCCGTTATCGATACCATA 208 653 213 Antisense
GAACAGCTGTTTCTCAGGCTATTGA 100 353 335 Antisense
GCACCTATCATGTTTACCAATCCTG 395 315 361 Antisense
GAACTCTCAAACAAGCTATCATCAG 433 349 398 Antisense
CAGTTGCGGTAAACACGCGGTCCGG 38 189 420 Antisense
TCCGGCTTCAACAATTCGTTTTTAT 236 595 440 Antisense
GGATCCCAATGATCTTTCTTCATTC 151 513 465 Antisense
ATTCTTGATCACAATGCCTTACATT 598 11 498 Antisense
Sequence Source Affymetrix Proprietary Database