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[185315_at]

Description H16O14.1 /REP_DB=WormBase Gene ID /WP=CE25033 /TR=Q9N5M5 /GB=AAF39883.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 52      6grp_high1_10_10 > 25      4grp_highest_8_8 > 52      4grp_high_8_8 > 16      6grp_highest_nonorm > 51      6grp_total > 25      4grp_total > 31      6grp_highest_sub > 01_02      6grp_high1_sub > 01_02      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 651
1 2 3 4 range
A 743.6
(3)
P 		162.3
(-3)
P 		199.3
(-2.6)
P 		339.7
(-1.2)
P 		581
B 673
(3)
P 		270.9
(-3)
P 		290.6
(-2.7)
P 		494.8
(0.3)
P 		402
C 813.7
(3)
P 		293.6
(-2.7)
P 		264.9
(-3)
P 		454.5
(-0.9)
P 		549
avr 743.4
(3) 		242.3
(-3) 		251.6
(-2.9) 		429.7
(-0.8) 		501

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 663
1 2 3 4 5 6 range
A 593.9
(3)
P 		535.9
(1.9)
P 		562.8
(2.4)
P 		280
(-3)
P 		541.4
(2)
P 		377.7
(-1.1)
P 		314
B 942.9
(3)
P 		855
(1.9)
P 		644.1
(-0.9)
P 		500.1
(-2.7)
P 		479
(-3)
P 		490.2
(-2.9)
P 		464
C 636.6
(0.5)
P 		837
(3)
P 		351.7
(-3)
P 		535.4
(-0.7)
P 		408.5
(-2.3)
P 		519.9
(-0.9)
P 		485
avr 724.5
(2.6) 		742.6
(3) 		519.5
(-1.4) 		438.5
(-3) 		476.3
(-2.3) 		462.6
(-2.5) 		304
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 236
1 2 3 4 range
A 270.7
(3)
P 		35.2
(-3)
P 		52.1
(-2.6)
P 		49.7
(-2.6)
P 		236
B 214.6
(3)
P 		66.9
(-3)
P 		67.3
(-3)
P 		105.4
(-1.4)
P 		148
C 269.4
(3)
P 		84.4
(-2.8)
P 		92.3
(-2.5)
P 		76.6
(-3)
P 		193
avr 251.6
(3) 		62.2
(-3) 		70.6
(-2.7) 		77.2
(-2.5) 		189

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 257
1 2 3 4 5 6 range
A 109.5
(-1.1)
P 		190.6
(3)
P 		122.6
(-0.4)
P 		71
(-3)
P 		163.4
(1.6)
P 		92
(-1.9)
P 		120
B 328.1
(3)
P 		284.6
(1.7)
P 		156.4
(-2.3)
P 		132.5
(-3)
P 		194.6
(-1.1)
P 		182.8
(-1.5)
P 		196
C 236
(2.3)
P 		255.4
(3)
P 		89.5
(-3)
P 		107.7
(-2.3)
P 		132.2
(-1.5)
P 		174
(0.1)
P 		166
avr 224.5
(2.2) 		243.5
(3) 		122.8
(-2.2) 		103.7
(-3) 		163.4
(-0.4) 		149.6
(-1) 		140
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185315_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10640
Sequence Type Exemplar sequence
Representative Public ID H16O14.1 NCBI
Target Description H16O14.1 /REP_DB=WormBase Gene ID /WP=CE25033 /TR=Q9N5M5 /GB=AAF39883.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068509(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068509 NCBI Caenorhabditis elegans H16O14.1 (H16O14.1) mRNA, complete cds. 11/11 None
SNAP00000027724 ENSEMBL cdna:SNAP chromosome:CEL140:IV:6065501:6073948:1 11/11 None
GENEFINDER00000027727 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:6053782:6073948:1 11/11 None
H16O14.1 ENSEMBL cdna:known chromosome:CEL140:IV:6065501:6074486:1 gene:H16O14.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:6065506-6073954(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl H16O14.1
Entrez Gene 177370 Entrez gene
SwissProt Q9N5M5 EMBL-EBI
WormBase CE29975 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1600968_AT similar to Solute carrier family 12, member 6 (Electroneutral potassium-chloride cotransporter 3) (K-Cl cotransporter 3) cfa
CANINE:1595162_AT similar to Solute carrier family 12, member 6 (Electroneutral potassium-chloride cotransporter 3) (K-Cl cotransporter 3) cfa
CANINE_2:CFA.18755.1.S1_AT similar to Solute carrier family 12, member 6 (Electroneutral potassium-chloride cotransporter 3) (K-Cl cotransporter 3) cfa
CANINE_2:CFAAFFX.12744.1.S1_S_AT similar to Solute carrier family 12, member 6 (Electroneutral potassium-chloride cotransporter 3) (K-Cl cotransporter 3) cfa
CANINE_2:CFAAFFX.12747.1.S1_S_AT similar to Solute carrier family 12, member 6 (Electroneutral potassium-chloride cotransporter 3) (K-Cl cotransporter 3) cfa
DROSOPHILA_2:1623037_A_AT dm
DROSGENOME1:152231_AT dm
DROSGENOME1:147816_AT dm
HG-U133_PLUS_2:220740_S_AT solute carrier family 12 (potassium/chloride transporters), member 6 hs
HG-U133A:220740_S_AT solute carrier family 12 (potassium/chloride transporters), member 6 hs
HG-FOCUS:220740_S_AT solute carrier family 12 (potassium/chloride transporters), member 6 hs
HG-U133A_2:220740_S_AT solute carrier family 12 (potassium/chloride transporters), member 6 hs
HG-U133_PLUS_2:223596_AT solute carrier family 12 (potassium/chloride transporters), member 6 hs
HG-U133B:223596_AT solute carrier family 12 (potassium/chloride transporters), member 6 hs
U133_X3P:G4826779_3P_A_AT solute carrier family 12 (potassium/chloride transporters), member 6 hs
HG-U95B:45662_AT solute carrier family 12 (potassium/chloride transporters), member 6 hs
HU35KSUBC:RC_N68456_AT Solute carrier family 12 (potassium/chloride transporters), member 6 hs
MOUSE430_2:1449878_A_AT solute carrier family 12, member 6 mm
MOE430A:1449878_A_AT solute carrier family 12, member 6 mm
MOUSE430A_2:1449878_A_AT solute carrier family 12, member 6 mm
MG-U74AV2:94035_AT solute carrier family 12, member 6 mm
MG-U74CV2:139199_AT solute carrier family 12, member 6 mm
MOE430A:1436989_S_AT solute carrier family 12, member 6 mm
MOUSE430A_2:1436989_S_AT solute carrier family 12, member 6 mm
MOUSE430_2:1436989_S_AT solute carrier family 12, member 6 mm
MU19KSUBC:TC35026_AT Solute carrier family 12, member 6 (Slc12a6), transcript variant 1, mRNA mm
MU19KSUBC:TC37172_AT Solute carrier family 12, member 6 (Slc12a6), transcript variant 1, mRNA mm
RAE230A:1374885_AT Solute carrier family 12, member 6 (predicted) rn
RAT230_2:1374885_AT Solute carrier family 12, member 6 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF39883 Hypothetical protein H16O14.1 [Caenorhabditis elegans] ref|NP_500910.2| H16O14.1 [Caenorhabditis elegans] 0.0
blast CAE61609 Hypothetical protein CBG05529 [Caenorhabditis briggsae] 0.0
blast XP_394587 PREDICTED: similar to GA18994-PA [Apis mellifera] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004841 EMBL-EBI Amino acid permease-associated region 5.0E-18
Pfam IPR004841 EMBL-EBI Amino acid permease-associated region 2.2E-21
Pfam IPR004841 EMBL-EBI Amino acid permease-associated region 5.0E-18
Pfam IPR004841 EMBL-EBI Amino acid permease-associated region 2.2E-21
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500910.2 10 5-27,36-58,90-112,125-147,151-173,294-313,333-355,432-451,456-478,490-512
H16O14.1 10 5-27,36-58,90-112,125-147,151-173,294-313,333-355,432-451,456-478,490-512
SNAP00000027724 10 5-27,36-58,90-112,125-147,151-173,294-313,333-355,432-451,456-478,490-512
GENEFINDER00000027727 11 107-129,139-161,182-204,224-246,253-275,396-418,439-461,481-503,534-553,558-580,592-614
Sequence
Target Sequence 
>C. ELEGANS:185315_AT
gactgactcggatatctctgactacacttacgaacgtaccatgaaaatggaggaacgtaa
tcaatacctgaaaaatttgaacaagtcggatcgtgataaggatattcagaaccatttgga
aattgtaacacgggaacgaaagctgagtagaattaatgaagaagcgcctgctgtggtccc
tgagcaacgtaacttggaggttgttgatgaagagcaagaagatggaaaatctgaaaatgg
ctcagcaaaaatcgagcacaaaggagtacgcttctcagatgatgaagattctaaggaggt
aaaagtcggaaacggaacgttggaacgcgatcgagaagaacggcaacgcaaacgacgata
caatgtgcataaaatgcatacagctgtcaaattgaacgagttgatgagacaaaaatcaag
tgatgctcagctggtatttgtgaatcttccaggaccacctgatgctgattctgattctta
ttatatggactttatcgaagctctcaccgaaggccttgaccgtgttttactg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACTGACTCGGATATCTCTGACTAC 177 375 2385 Antisense
CTCTGACTACACTTACGAACGTACC 601 229 2400 Antisense
AATGAAGAAGCGCCTGCTGTGGTCC 297 163 2539 Antisense
TGGTCCCTGAGCAACGTAACTTGGA 671 539 2558 Antisense
GAGCACAAAGGAGTACGCTTCTCAG 309 389 2638 Antisense
GAACGGCAACGCAAACGACGATACA 272 347 2722 Antisense
GATGCTCAGCTGGTATTTGTGAATC 612 413 2806 Antisense
TTTGTGAATCTTCCAGGACCACCTG 209 667 2821 Antisense
GACCACCTGATGCTGATTCTGATTC 166 385 2837 Antisense
GACTTTATCGAAGCTCTCACCGAAG 598 371 2872 Antisense
CGAAGGCCTTGACCGTGTTTTACTG 71 243 2892 Antisense
Sequence Source Affymetrix Proprietary Database