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[185307_at]

Description Y113G7B.2 /REP_DB=WormBase Gene ID /WP=CE23285 /TR=Q9U2Y0 /GB=CAB54325.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 06      6grp_high1_10_10 > 60      4grp_highest_8_8 > 0      4grp_high_8_8 > 36      6grp_highest_nonorm > 90      6grp_total > 60      4grp_total > 43      6grp_highest_sub > 04      6grp_cgc > 95      6grp_high1_sub > 04      6grp_cgc_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 217
1 2 3 4 range
A 131
(3)
P 		101.3
(-3)
P 		118.5
(0.5)
P 		124.9
(1.8)
P 		30
B 122.2
(-0.7)
P 		169
(3)
P 		128.3
(-0.2)
P 		92.4
(-3)
P 		77
C 133.9
(-1.8)
P 		306
(3)
P 		122.4
(-2.1)
P 		89.1
(-3)
P 		217
avr 129
(-1.2) 		192.1
(3) 		123.1
(-1.6) 		102.1
(-3) 		90

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 87
1 2 3 4 5 6 range
A 122
(-0.3)
P 		102.1
(-2.4)
P 		95.7
(-3)
P 		143.3
(1.8)
P 		154.9
(3)
P 		116.8
(-0.9)
P 		59
B 125.1
(2.4)
P 		123.9
(2.1)
P 		110.2
(-1.2)
P 		127.5
(3)
P 		102.7
(-3)
P 		125.4
(2.5)
P 		25
C 116.2
(0.5)
P 		135.3
(1.9)
P 		150.6
(3)
P 		129.3
(1.5)
P 		67.9
(-3)
P 		91.3
(-1.3)
P 		83
avr 121.1
(0) 		120.4
(-0.1) 		118.8
(-0.5) 		133.4
(3) 		108.5
(-3) 		111.2
(-2.3) 		25
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 73
1 2 3 4 range
A 47.7
(3)
P 		22
(-2.2)
P 		31
(-0.4)
P 		18.3
(-3)
P 		29
B 39
(2.3)
P 		41.7
(3)
P 		29.7
(-0.3)
P 		19.7
(-3)
P 		22
C 44.3
(-0.6)
P 		88
(3)
P 		42.7
(-0.7)
P 		15
(-3)
P 		73
avr 43.7
(1.7) 		50.6
(3) 		34.5
(0.1) 		17.7
(-3) 		33

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 26
1 2 3 4 5 6 range
A 22.5
(-2.6)
P 		36.3
(0.6)
P 		20.8
(-3)
P 		36.3
(0.6)
P 		46.7
(3)
P 		28.4
(-1.2)
P 		26
B 43.5
(2)
P 		41.2
(1.3)
P 		26.7
(-3)
P 		33.8
(-0.9)
P 		41.7
(1.5)
P 		46.8
(3)
P 		20
C 43.1
(3)
P 		41.3
(2.5)
P 		38.3
(1.6)
P 		26
(-1.9)
P 		22
(-3)
P 		30.6
(-0.6)
P 		21
avr 36.4
(1.3) 		39.6
(3) 		28.6
(-3) 		32
(-1.1) 		36.8
(1.5) 		35.3
(0.7) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185307_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13456
Sequence Type Exemplar sequence
Representative Public ID Y113G7B.2 NCBI
Target Description Y113G7B.2 /REP_DB=WormBase Gene ID /WP=CE23285 /TR=Q9U2Y0 /GB=CAB54325.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075493(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075493 NCBI Caenorhabditis elegans Serpentine Receptor, class H family member (srh-217) (srh-217) mRNA, complete cds. 11/11 None
SNAP00000024707 ENSEMBL cdna:SNAP chromosome:CEL140:V:20191460:20193243:-1 11/11 None
Y113G7B.2 ENSEMBL cdna:known chromosome:CEL140:V:20191460:20193243:-1 gene:Y113G7B.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000024730 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:20191459-20193243(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol srh-217
Ensembl Y113G7B.2
Entrez Gene 190969 Entrez gene
SwissProt Q9U2Y0 EMBL-EBI
WormBase CE23285 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4930 G-protein coupled receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB54325 Hypothetical protein Y113G7B.2 [Caenorhabditis elegans] ref|NP_507894.1| Serpentine Receptor, class H family member (srh-217) [Caenorhabditis elegans] 1.0E-175
blast CAB54325 Hypothetical protein Y113G7B.2 [Caenorhabditis elegans] ref|NP_507894.1| Serpentine Receptor, class H family member (srh-217) [Caenorhabditis elegans] 1.0E-155
blast CAB04743 Hypothetical protein T20B3.5 [Caenorhabditis elegans] ref|NP_507246.1| Serpentine Receptor, class H family member (srh-216) [Caenorhabditis elegans] 1.0E-127
blast CAB04741 Hypothetical protein T20B3.3 [Caenorhabditis elegans] ref|NP_507248.1| Serpentine Receptor, class H family member (srh-215) [Caenorhabditis elegans] 1.0E-109
blast CAB04743 Hypothetical protein T20B3.5 [Caenorhabditis elegans] ref|NP_507246.1| Serpentine Receptor, class H family member (srh-216) [Caenorhabditis elegans] 1.0E-108
blast CAB04741 Hypothetical protein T20B3.3 [Caenorhabditis elegans] ref|NP_507248.1| Serpentine Receptor, class H family member (srh-215) [Caenorhabditis elegans] 1.0E-92
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 9.5E-29
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 2.0E-105
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507894.1 7 20-42,63-85,100-119,136-158,193-215,242-264,274-296
Y113G7B.2 7 20-42,63-85,100-119,136-158,193-215,242-264,274-296
SNAP00000024707 6 20-42,74-96,109-131,166-188,215-237,247-269
Sequence
Target Sequence 
>C. ELEGANS:185307_AT
taaaaaactccaccgatacgccatcccaacctacatcctccactacatcttcccattagt
cgtcgttccccaaataatcaacattcctgaccaggaagcaggtcggaataattttcagca
aaaattcggatgtattcctccgtacgttgaaattgatagaatgttctatttgacaatgaa
aaaacggtatttcctaatcacatgtgctgtattcatctgtataacgtttgccgaggtgtg
gttgtttgcatttattacggataggttgattaagaagcaggtgcaaggatccatgtctca
gaacactgccgcagtgcacagaaaatttcagagagcgtttatattacagattctcatccc
attcgtaattgtattccttcctatcttctatatcggagttacttgtatatcggagtatca
caatcagttcttcaataacatcactattattataaactcatcacatggattcttctcgac
aattgctatgattgctcttcatgctccatatcggaactacaccagaaagttgctaccatc
atgccgacgattcaactctgtggt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAAAAAACTCCACCGATACGCCATC 493 639 402 Antisense
GTCGTCGTTCCCCAAATAATCAACA 527 471 460 Antisense
GATGTATTCCTCCGTACGTTGAAAT 505 407 530 Antisense
GGTATTTCCTAATCACATGTGCTGT 157 505 587 Antisense
GATCCATGTCTCAGAACACTGCCGC 40 417 689 Antisense
ATTACAGATTCTCATCCCATTCGTA 406 13 744 Antisense
ATTCCTTCCTATCTTCTATATCGGA 257 7 774 Antisense
ACTCATCACATGGATTCTTCTCGAC 366 99 857 Antisense
TGCTCTTCATGCTCCATATCGGAAC 252 581 894 Antisense
AGAAAGTTGCTACCATCATGCCGAC 667 71 925 Antisense
CATGCCGACGATTCAACTCTGTGGT 208 211 941 Antisense
Sequence Source Affymetrix Proprietary Database