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[185305_at]

Description Y54H5A.3 /REP_DB=WormBase Gene ID /WP=CE24459 /TR=Q9N394 /GB=AAF60781.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 98      6grp_high1_10_10 > 78      4grp_highest_8_8 > 27      4grp_high_8_8 > 56      6grp_highest_nonorm > 84      6grp_total > 59      4grp_total > 07      6grp_highest_sub > Cu      6grp_cgc > 39      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 260
1 2 3 4 range
A 648.6
(3)
P 		431.8
(-3)
P 		544.6
(0.1)
P 		465.2
(-2.1)
P 		217
B 456.5
(-3)
P 		485.4
(-1.4)
P 		562
(3)
P 		520.7
(0.7)
P 		106
C 659.9
(2.2)
P 		452
(-3)
P 		692
(3)
P 		618.3
(1.2)
P 		240
avr 588.3
(2.5) 		456.4
(-3) 		599.5
(3) 		534.7
(0.3) 		143

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 376
1 2 3 4 5 6 range
A 562
(-1.8)
P 		500.9
(-3)
P 		554.1
(-2)
P 		538.1
(-2.3)
P 		585.7
(-1.4)
P 		814.2
(3)
P 		313
B 836.9
(3)
P 		546.1
(-1.9)
P 		481.2
(-3)
P 		750.4
(1.5)
P 		662.1
(0.1)
P 		752.7
(1.6)
P 		356
C 640.3
(0.7)
P 		461.3
(-3)
P 		488.1
(-2.4)
P 		679.5
(1.6)
P 		721.6
(2.4)
P 		748.4
(3)
P 		287
avr 679.7
(0.9) 		502.8
(-3) 		507.8
(-2.9) 		656
(0.4) 		656.5
(0.4) 		771.8
(3) 		269
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 173
1 2 3 4 range
A 236.2
(3)
P 		93.7
(-2.1)
P 		142.4
(-0.3)
P 		68.1
(-3)
P 		168
B 145.6
(3)
P 		119.9
(-1.5)
P 		130.2
(0.3)
P 		111
(-3)
P 		35
C 218.4
(2)
P 		129.9
(-1.9)
P 		241.2
(3)
P 		104.3
(-3)
P 		137
avr 200.1
(3) 		114.5
(-1.9) 		171.3
(1.4) 		94.5
(-3) 		106

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 188
1 2 3 4 5 6 range
A 103.6
(-3)
P 		178.2
(1.7)
P 		120.7
(-1.9)
P 		136.5
(-0.9)
P 		176.7
(1.6)
P 		198.2
(3)
P 		95
B 291.2
(3)
P 		181.8
(-0.8)
P 		116.9
(-3)
P 		198.7
(-0.2)
P 		269
(2.2)
P 		280.7
(2.6)
P 		174
C 237.3
(2.4)
P 		140.8
(-2.2)
P 		124.2
(-3)
P 		136.6
(-2.4)
P 		233.5
(2.2)
P 		250.5
(3)
P 		126
avr 210.7
(1.4) 		166.9
(-0.7) 		120.6
(-3) 		157.3
(-1.2) 		226.4
(2.2) 		243.1
(3) 		123
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185305_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7363
Sequence Type Exemplar sequence
Representative Public ID Y54H5A.3 NCBI
Target Description Y54H5A.3 /REP_DB=WormBase Gene ID /WP=CE24459 /TR=Q9N394 /GB=AAF60781.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065689(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065689 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-262) (tag-262) mRNA, complete cds. 11/11 None
SNAP00000038483 ENSEMBL cdna:SNAP chromosome:CEL140:III:5151998:5153084:-1 11/11 None
GENEFINDER00000038489 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5151998:5153084:-1 11/11 None
Y54H5A.3 ENSEMBL cdna:known chromosome:CEL140:III:5151998:5153111:-1 gene:Y54H5A.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5151996-5153083(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol tag-262
Ensembl Y54H5A.3
Entrez Gene 175700 Entrez gene
SwissProt Q9N394 EMBL-EBI
WormBase CE24459 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF60781 Temporarily assigned gene name protein 262 [Caenorhabditis elegans] ref|NP_498090.1| Temporarily Assigned Gene name family member (tag-262) [Caenorhabditis elegans] 1.0E-120
blast CAE73769 Hypothetical protein CBG21313 [Caenorhabditis briggsae] 1.0E-106
blast NP_001007900 MGC79565 protein [Xenopus tropicalis] gb|AAH80336.1| MGC79565 protein [Xenopus tropicalis] 2.0E-47
Protein Domains
Database ID Description E-Value
Pfam IPR000504 EMBL-EBI RNA-binding region RNP-1 (RNA recognition motif) 5.9E-22
Sequence
Target Sequence 
>C. ELEGANS:185305_AT
ttcaattttcatgcctccacaattaagagctgcaccgataatgttccctcctcgagtact
cggaattccggctcctgcaactattgaaggtgctccagcattatatgaaacacctgcagt
aaacgcaattccacaagaacttctgcgaactcaagctcttcaatcagatattgataagat
gaatttcaaaaagcaaaccgatccatttaagaagaaaatcaaggaacaacaagcgaaaaa
gaagtttgtgagatccggtggtggtcaggtttgggaagatccatctcttgccgaatggga
tgaaaatgattttcgagttttctgtggagatctgggaaatgaagtgtcggatgaacttct
tgcaaaggcttttcgaaaatacccaagtttccaaaaagccaaagttgtcagagaatcacg
aacaaataagagtaaaggatatggattcgtttcattcagagattcgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCAATTTTCATGCCTCCACAATTA 447 685 309 Antisense
AAGAGCTGCACCGATAATGTTCCCT 180 155 333 Antisense
TCGAGTACTCGGAATTCCGGCTCCT 235 603 360 Antisense
TTCCGGCTCCTGCAACTATTGAAGG 8 701 374 Antisense
ACACCTGCAGTAAACGCAATTCCAC 679 107 418 Antisense
ATTCCACAAGAACTTCTGCGAACTC 690 5 436 Antisense
CTGCGAACTCAAGCTCTTCAATCAG 692 233 451 Antisense
GAGATCCGGTGGTGGTCAGGTTTGG 240 389 558 Antisense
TTGGGAAGATCCATCTCTTGCCGAA 230 709 579 Antisense
TGAACTTCTTGCAAAGGCTTTTCGA 106 573 660 Antisense
GGATTCGTTTCATTCAGAGATTCGG 346 509 751 Antisense
Sequence Source Affymetrix Proprietary Database