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[185287_at]

Description Y53F4B.25 /REP_DB=WormBase Gene ID /WP=CE26158 /TR=Q9NA94 /GB=CAB70112.2 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 10      4grp_total > 49      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 325
1 2 3 4 range
A 30.5
(-2.8)
A 		276.7
(1.8)
P 		343.6
(3)
P 		19.1
(-3)
A 		325
B 69.9
(-2.5)
A 		292.2
(3)
P 		273.4
(2.5)
P 		50.5
(-3)
A 		242
C 85.7
(-2.4)
A 		294.3
(3)
P 		230
(1.3)
P 		64.2
(-3)
A 		230
avr 62
(-2.6) 		287.7
(3) 		282.3
(2.9) 		44.6
(-3) 		243

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 258
1 2 3 4 5 6 range
A 61
(-3)
A 		212.2
(3)
P 		123
(-0.5)
P 		149.6
(0.5)
P 		165.9
(1.2)
P 		77.9
(-2.3)
P 		151
B 62
(-3)
A 		252
(3)
P 		151
(-0.2)
M 		160.7
(0.1)
P 		149.8
(-0.2)
P 		72.7
(-2.7)
P 		190
C 63.5
(-3)
A 		319.2
(3)
P 		190.7
(-0)
P 		144.9
(-1.1)
P 		203.8
(0.3)
P 		70.7
(-2.8)
P 		256
avr 62.2
(-3) 		261.1
(3) 		154.9
(-0.2) 		151.7
(-0.3) 		173.2
(0.3) 		73.8
(-2.7) 		199
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 87
1 2 3 4 range
A 11.1
(-2.4)
A 		60.1
(1)
P 		89.8
(3)
P 		2.8
(-3)
A 		87
B 22.3
(-1.9)
A 		72.2
(3)
P 		63.4
(2.1)
P 		10.8
(-3)
A 		61
C 28.4
(-1.6)
A 		84.6
(3)
P 		80.2
(2.6)
P 		10.8
(-3)
A 		74
avr 20.6
(-1.9) 		72.3
(2.5) 		77.8
(3) 		8.1
(-3) 		70

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 86
1 2 3 4 5 6 range
A 11.2
(-3)
A 		75.5
(3)
P 		26.8
(-1.5)
P 		37.9
(-0.5)
P 		50
(0.6)
P 		19
(-2.3)
P 		64
B 21.6
(-3)
A 		83.9
(3)
P 		36.7
(-1.5)
M 		42.6
(-1)
P 		60.8
(0.8)
P 		27.1
(-2.5)
P 		62
C 23.5
(-3)
A 		97.4
(3)
P 		48.5
(-1)
P 		29.1
(-2.5)
P 		66
(0.5)
P 		23.7
(-3)
P 		74
avr 18.8
(-3) 		85.6
(3) 		37.3
(-1.3) 		36.5
(-1.4) 		58.9
(0.6) 		23.3
(-2.6) 		67
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185287_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3653
Sequence Type Exemplar sequence
Representative Public ID Y53F4B.25 NCBI
Target Description Y53F4B.25 /REP_DB=WormBase Gene ID /WP=CE26158 /TR=Q9NA94 /GB=CAB70112.2 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064710(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064710 NCBI Caenorhabditis elegans Y53F4B.25 (Y53F4B.25) mRNA, complete cds. 11/11 None
SNAP00000043064 ENSEMBL cdna:SNAP chromosome:CEL140:II:15141613:15151733:1 11/11 None
GENEFINDER00000043150 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:15141227:15151733:1 11/11 None
Y53F4B.25 ENSEMBL cdna:known chromosome:CEL140:II:15135383:15145163:1 gene:Y53F4B.25 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:15135379-15145137(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y53F4B.25
Entrez Gene 175163 Entrez gene
SwissProt Q9NA94 EMBL-EBI
WormBase CE26158 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB70112 Hypothetical protein Y53F4B.25 [Caenorhabditis elegans] ref|NP_497111.1| Y53F4B.25 [Caenorhabditis elegans] 0.0
blast CAE59729 Hypothetical protein CBG03165 [Caenorhabditis briggsae] 0.0
blast CAB61101 Hypothetical protein Y53F4B.27a [Caenorhabditis elegans] ref|NP_001022467.1| Y53F4B.27a [Caenorhabditis elegans] 0.0
blast CAE59728 Hypothetical protein CBG03164 [Caenorhabditis briggsae] 0.0
blast CAI70419 Hypothetical protein Y53F4B.27b [Caenorhabditis elegans] ref|NP_001022468.1| Y53F4B.27b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002602 EMBL-EBI Protein of unknown function DB 6.5E-13
Pfam IPR002602 EMBL-EBI Protein of unknown function DB 3.5E-16
Pfam IPR002602 EMBL-EBI Protein of unknown function DB 5.7E-19
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497111.1 2 4-26,35-57
Y53F4B.25 2 4-26,35-57
Sequence
Target Sequence 
>C. ELEGANS:185287_AT
cagcagtttttcagatcaatccccgaaaaaacctccgaacaactccgaaactttgtaaaa
gtccatccccaagtgatgaacaacctccagaagcctctgatctaccgtggaaaagaaatg
gtgattccgaaaatgttcaacttcaccaccaaccccacacctccaccaagattaccccag
cctccgacggctccgccaactgttccacctccaccaaatcaggctccaatagttcctcat
cagcctatgtacgtcccattaatcaatccgaatcagaagttggacttgtgttgtcgcaag
cagaggatttcaccgttgtgccagaatttgtgcaactttgatacatttaatgataaaact
ttggtagccgctttcctaaccaaccaatgccctggacctcaactcggtcaagcttacgac
tgtgcctcctcaaaagccgaccactctgcatgctgtgaacgtgctggaattctatcgttc
caaggcggaaaatgtattccattctgtcgaacccacgtggcaactccttcaaatgttttc
g

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAGCAGTTTTTCAGATCAATCCCCG 557 201 1195 Antisense
ATGAACAACCTCCAGAAGCCTCTGA 633 43 1270 Antisense
AAGCCTCTGATCTACCGTGGAAAAG 1 145 1285 Antisense
CGAAAATGTTCAACTTCACCACCAA 208 253 1322 Antisense
CCTCCACCAAATCAGGCTCCAATAG 632 265 1402 Antisense
GGCTCCAATAGTTCCTCATCAGCCT 697 537 1416 Antisense
TGTACGTCCCATTAATCAATCCGAA 402 559 1442 Antisense
GAGGATTTCACCGTTGTGCCAGAAT 85 403 1497 Antisense
TCGGTCAAGCTTACGACTGTGCCTC 371 605 1598 Antisense
CGACCACTCTGCATGCTGTGAACGT 516 249 1632 Antisense
GTGGCAACTCCTTCAAATGTTTTCG 515 491 1711 Antisense
Sequence Source Affymetrix Proprietary Database