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[185281_at]

Description F40E12.2 /REP_DB=WormBase Gene ID /WP=CE25879 /TR=O16568 /GB=AAB66047.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 83      4grp_total > 45      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 41
1 2 3 4 range
A 21.4
(-0.6)
A 		46.1
(3)
A 		4.9
(-3)
A 		14.8
(-1.6)
A 		41
B 42.9
(3)
A 		12.3
(-2.1)
A 		25.4
(0.1)
A 		6.6
(-3)
A 		36
C 16.6
(-1.4)
A 		23.2
(3)
A 		16.6
(-1.4)
A 		14.1
(-3)
A 		9
avr 27
(2.9) 		27.2
(3) 		15.6
(-1.5) 		11.8
(-3) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 11.3
(-2.2)
A 		11.7
(-2.2)
A 		19
(-0.8)
A 		39.8
(3)
A 		25.9
(0.4)
A 		7.2
(-3)
A 		33
B 14.9
(-0.3)
A 		8.5
(-3)
A 		9.1
(-2.7)
A 		12.1
(-1.5)
A 		22.5
(3)
A 		19.2
(1.6)
A 		14
C 16.9
(-0.3)
A 		15.8
(-0.6)
A 		29.5
(3)
A 		16.6
(-0.4)
A 		27.7
(2.5)
A 		6.4
(-3)
A 		23
avr 14.4
(-1.6) 		12
(-2.5) 		19.2
(0.4) 		22.8
(1.9) 		25.4
(3) 		10.9
(-3) 		15
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 7.8
(1.5)
A 		10
(3)
A 		1.3
(-3)
A 		2.2
(-2.4)
A 		9
B 13.7
(3)
A 		3
(-2.2)
A 		5.9
(-0.8)
A 		1.4
(-3)
A 		12
C 5.5
(1.3)
A 		6.7
(3)
A 		5.8
(1.7)
A 		2.4
(-3)
A 		4
avr 9
(3) 		6.6
(0.9) 		4.3
(-1) 		2
(-3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8
1 2 3 4 5 6 range
A 2.1
(-2.8)
A 		4.1
(-1.3)
A 		4.1
(-1.3)
A 		10.1
(3)
A 		7.8
(1.3)
A 		1.8
(-3)
A 		8
B 5.2
(-0.4)
A 		2.8
(-2.5)
A 		2.2
(-3)
A 		3.2
(-2.1)
A 		9.1
(3)
A 		7.2
(1.3)
A 		7
C 6.3
(0.7)
A 		4.8
(-0.7)
A 		7.5
(1.7)
A 		3.3
(-2)
A 		9
(3)
A 		2.1
(-3)
A 		7
avr 4.5
(-2) 		3.9
(-2.8) 		4.6
(-1.9) 		5.5
(-0.8) 		8.6
(3) 		3.7
(-3) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185281_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5523
Sequence Type Exemplar sequence
Representative Public ID F40E12.2 NCBI
Target Description F40E12.2 /REP_DB=WormBase Gene ID /WP=CE25879 /TR=O16568 /GB=AAB66047.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062194(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062194 NCBI Caenorhabditis elegans F40E12.2 (F40E12.2) mRNA, complete cds. 11/11 None
SNAP00000029602 ENSEMBL cdna:SNAP chromosome:CEL140:II:3610018:3615346:1 11/11 None
GENEFINDER00000029605 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3610042:3615346:1 11/11 None
F40E12.2 ENSEMBL cdna:known chromosome:CEL140:II:3610403:3615346:1 gene:F40E12.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3610973-3615345(+) 97.7 97.7
Public Domain and Genome References
Gene Title
Ensembl F40E12.2
Entrez Gene 185537 Entrez gene
SwissProt O16568 EMBL-EBI
WormBase CE32657 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB66047 Hypothetical protein F40E12.2 [Caenorhabditis elegans] ref|NP_494595.2| F40E12.2 [Caenorhabditis elegans] 0.0
blast G88104 protein F40E12.2 [imported] - Caenorhabditis elegans 0.0
blast AAC19195 Hypothetical protein F56H1.2 [Caenorhabditis elegans] ref|NP_491671.1| F56H1.2 [Caenorhabditis elegans] 3.0E-56
Protein Domains
Database ID Description E-Value
Pfam IPR003125 EMBL-EBI Protein of unknown function WSN 5.8E-23
Pfam IPR001357 EMBL-EBI BRCT 1.0E-13
Pfam IPR002110 EMBL-EBI Ankyrin 1.6E-7
Pfam IPR003125 EMBL-EBI Protein of unknown function WSN 9.1E-19
Pfam IPR001357 EMBL-EBI BRCT 1.0E-13
Pfam IPR002110 EMBL-EBI Ankyrin 1.6E-7
Pfam IPR003125 EMBL-EBI Protein of unknown function WSN 5.8E-23
Pfam IPR001357 EMBL-EBI BRCT 1.0E-13
Pfam IPR002110 EMBL-EBI Ankyrin 1.6E-7
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494595.2 2 741-763,981-1003
F40E12.2 2 741-763,981-1003
SNAP00000029602 2 709-731,949-971
GENEFINDER00000029605 2 736-758,976-998
Sequence
Target Sequence 
>C. ELEGANS:185281_AT
tttggcggctccgttgtgcaagatattgaagctgacgacgagaagactccgaagccatac
tggacgttgtctagcttctataagaagaagaagtgggagcagagaagctgcatcattctc
aagcactcggatagtaagattgaaaagaaatggcagtctatgcacgcaatgtataccttg
gtcgacttctcattccttgccgaatgcattgtccgtcactacatccttccgattgataac
gccgtcgtgccattggacagaatcgcaagtgataaggaagttgtcagaaaacgcgagaag
aagaaaaaagaggaggaggaccgaatcgcccgagcactgaagaccgagccgactgaagag
gagccaactgacccggaggatcttatcgtttatgatccgagaaagcaatacaagcccatg
aaggggaagaagaaggttcatccgatgaagataaagaagaaggcgcccaagaagaagaag
gaagggactctcactgaaaccac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTGGCGGCTCCGTTGTGCAAGATA 445 665 3304 Antisense
GACTCCGAAGCCATACTGGACGTTG 114 373 3348 Antisense
GCATCATTCTCAAGCACTCGGATAG 565 309 3413 Antisense
ATGTATACCTTGGTCGACTTCTCAT 43 47 3472 Antisense
TCCTTGCCGAATGCATTGTCCGTCA 684 591 3497 Antisense
CATCCTTCCGATTGATAACGCCGTC 70 211 3525 Antisense
CGCCGTCGTGCCATTGGACAGAATC 357 257 3543 Antisense
AAGAGGAGGAGGACCGAATCGCCCG 655 153 3611 Antisense
GACCGAGCCGACTGAAGAGGAGCCA 120 383 3645 Antisense
AACTGACCCGGAGGATCTTATCGTT 700 141 3669 Antisense
AGGAAGGGACTCTCACTGAAACCAC 387 39 3782 Antisense
Sequence Source Affymetrix Proprietary Database