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[185277_at]

Description K08D10.5 /REP_DB=WormBase Gene ID /WP=CE07357 /TR=Q21321 /GB=AAA98031.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 08      4grp_total > 61      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 27
1 2 3 4 range
A 27.8
(3)
A 		9.6
(-1.9)
A 		5.5
(-3)
A 		10.3
(-1.7)
A 		22
B 3.8
(-2.5)
A 		4
(-2.5)
A 		28.4
(3)
A 		1.8
(-3)
A 		27
C 2.7
(-2.4)
A 		11.6
(3)
A 		1.7
(-3)
A 		3.2
(-2.1)
A 		10
avr 11.4
(2.6) 		8.4
(-0.1) 		11.9
(3) 		5.1
(-3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 51
1 2 3 4 5 6 range
A 52.6
(3)
A 		26.8
(-0.7)
A 		26.2
(-0.8)
A 		10.4
(-3)
A 		18.4
(-1.9)
A 		22.6
(-1.3)
A 		42
B 7.7
(0.4)
A 		4.7
(-1.5)
A 		12
(3)
A 		11.8
(2.9)
A 		3
(-2.5)
A 		2.2
(-3)
A 		10
C 21.8
(3)
A 		1.8
(-3)
A 		7.4
(-1.3)
A 		16.2
(1.3)
A 		4.7
(-2.1)
A 		4.7
(-2.1)
A 		20
avr 27.4
(3) 		11.1
(-2.2) 		15.2
(-0.9) 		12.8
(-1.7) 		8.7
(-3) 		9.8
(-2.6) 		19
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10
1 2 3 4 range
A 10.1
(3)
A 		2.1
(-2.5)
A 		1.4
(-3)
A 		1.5
(-2.9)
A 		9
B 1.2
(-2.2)
A 		1
(-2.4)
A 		6.6
(3)
A 		0.4
(-3)
A 		6
C 0.9
(-2.1)
A 		3.3
(3)
A 		0.6
(-2.8)
A 		0.5
(-3)
A 		3
avr 4.1
(3) 		2.1
(-0.6) 		2.9
(0.8) 		0.8
(-3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9
1 2 3 4 5 6 range
A 9.7
(3)
A 		9.5
(2.8)
A 		5.7
(-0.4)
A 		2.6
(-3)
A 		5.6
(-0.5)
A 		5.5
(-0.5)
A 		7
B 2.7
(2)
A 		1.6
(-0.9)
A 		2.9
(2.5)
A 		3.1
(3)
A 		1.2
(-2)
A 		0.8
(-3)
A 		2
C 8.1
(3)
A 		0.5
(-3)
A 		1.9
(-1.9)
A 		3.3
(-0.8)
A 		1.5
(-2.2)
A 		1.6
(-2.1)
A 		8
avr 6.8
(3) 		3.9
(-1.1) 		3.5
(-1.7) 		3
(-2.4) 		2.8
(-2.7) 		2.6
(-3) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185277_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10953
Sequence Type Exemplar sequence
Representative Public ID K08D10.5 NCBI
Target Description K08D10.5 /REP_DB=WormBase Gene ID /WP=CE07357 /TR=Q21321 /GB=AAA98031.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068102(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068102 NCBI Caenorhabditis elegans K08D10.5 (K08D10.5) mRNA, complete cds. 11/11 None
SNAP00000009683 ENSEMBL cdna:SNAP chromosome:CEL140:IV:4166534:4169884:1 11/11 None
GENEFINDER00000009695 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:4166534:4169884:1 11/11 None
K08D10.5 ENSEMBL cdna:known chromosome:CEL140:IV:4168031:4169884:1 gene:K08D10.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:4168036-4169324(+) 43.67 68.71
Public Domain and Genome References
Gene Title
Ensembl K08D10.5
Entrez Gene 187150 Entrez gene
SwissProt Q21321 EMBL-EBI
WormBase CE07357 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA98031 Hypothetical protein K08D10.5 [Caenorhabditis elegans] ref|NP_500503.1| K08D10.5 [Caenorhabditis elegans] 0.0
blast CAA86856 Hypothetical protein R03D7.2 [Caenorhabditis elegans] ref|NP_496354.1| R03D7.2 [Caenorhabditis elegans] 1.0E-136
blast CAB04311 Hypothetical protein F46A8.10 [Caenorhabditis elegans] ref|NP_492890.2| F46A8.10 [Caenorhabditis elegans] 2.0E-69
blast CAE56861 Hypothetical protein CBG24691 [Caenorhabditis briggsae] 6.0E-64
Protein Domains
Database ID Description E-Value
Pfam IPR005552 EMBL-EBI Scramblase 7.9E-62
Pfam IPR005552 EMBL-EBI Scramblase 7.2E-53
Sequence
Target Sequence 
>C. ELEGANS:185277_AT
gatgatcacggacttactcggagcagtgttctacggaaacaccctcacttcaactaggaa
agccgtcaatcgagtaccgattctggaagccatggaacttcctagccatcacccgttcgt
cttcgtcaactacatttcgcagcagacgcaagtcggaaagagtttctcgaacgagaacga
agcgagatatgctctccagttggtcaccgctctgaaacgacacgcacgtgagcagaacac
caccgtttccgtcgcagttttgaatttctatggtgcacaatattcgtatgtgtattcgat
cgcagaggatggagttcgcgtgaacactatcgacggttgtcagggacaagagtacgatgt
ctccatcgtgcttttaacaagatcggatccttatgagaaatccaaattcctcgtgaatcc
gaatagaatcaacgtggcgctgagccgatctaaaatggctacagtagtcatcggacaacg
gcacctcatcggcaatcatccaaacgttcaacaagccaaacgtggacgttatcaacgctc
ttgcaactggtctcgacttctcgacaaacttccagctgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGATCACGGACTTACTCGGAGCA 250 411 1197 Antisense
GAAACACCCTCACTTCAACTAGGAA 22 357 1232 Antisense
GGAAAGCCGTCAATCGAGTACCGAT 119 527 1253 Antisense
GAAGCCATGGAACTTCCTAGCCATC 461 345 1282 Antisense
TCTTCGTCAACTACATTTCGCAGCA 490 617 1316 Antisense
GACACGCACGTGAGCAGAACACCAC 674 367 1415 Antisense
ATCAACGTGGCGCTGAGCCGATCTA 304 27 1624 Antisense
AGTAGTCATCGGACAACGGCACCTC 639 61 1659 Antisense
CACCTCATCGGCAATCATCCAAACG 662 201 1678 Antisense
ACGTTATCAACGCTCTTGCAACTGG 655 91 1722 Antisense
ACTTCTCGACAAACTTCCAGCTGAA 530 103 1752 Antisense
Sequence Source Affymetrix Proprietary Database