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[185272_at]

Description K08D10.2 /REP_DB=WormBase Gene ID /WP=CE07354 /TR=Q21324 /GB=AAA98034.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 67      6grp_high1_10_10 > 86      4grp_highest_8_8 > 39      4grp_high_8_8 > 20      6grp_highest_nonorm > 81      6grp_total > 66      4grp_total > 20      6grp_highest_sub > In      6grp_cgc > 47      6grp_high1_sub > In      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 141
1 2 3 4 range
A 231.2
(-1.5)
P 		295.3
(2.3)
P 		205.1
(-3)
P 		306.8
(3)
P 		102
B 243.4
(-1)
P 		208
(-2.5)
P 		196.1
(-3)
P 		337
(3)
P 		141
C 276.4
(1)
P 		247
(-1.1)
P 		219.7
(-3)
P 		304.6
(3)
P 		85
avr 250.3
(-0.6) 		250.1
(-0.6) 		207
(-3) 		316.1
(3) 		109

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 217
1 2 3 4 5 6 range
A 194.8
(-3)
P 		231.6
(-1.5)
P 		344
(3)
P 		240.3
(-1.2)
P 		229
(-1.6)
P 		294.5
(1)
P 		149
B 235.9
(-0.3)
P 		213.5
(-1.2)
P 		305
(2.6)
P 		169.9
(-3)
P 		271.3
(1.2)
P 		314
(3)
P 		144
C 265.7
(-1.2)
P 		249.1
(-1.8)
P 		212.9
(-3)
P 		258.2
(-1.4)
P 		253.3
(-1.6)
P 		387.1
(3)
P 		174
avr 232.1
(-2.5) 		231.4
(-2.5) 		287.3
(0.5) 		222.8
(-3) 		251.2
(-1.4) 		331.9
(3) 		109
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 47
1 2 3 4 range
A 84.2
(3)
P 		64.1
(-0.1)
P 		53.6
(-1.7)
P 		44.9
(-3)
P 		39
B 77.6
(3)
P 		51.4
(-1.9)
P 		45.4
(-3)
P 		71.8
(1.9)
P 		32
C 91.5
(3)
P 		71
(-0.1)
P 		76.6
(0.8)
P 		51.4
(-3)
P 		40
avr 84.4
(3) 		62.2
(-1.7) 		58.5
(-2.5) 		56
(-3) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 94
1 2 3 4 5 6 range
A 35.9
(-3)
P 		82.4
(3)
P 		74.9
(2)
P 		60.9
(0.2)
P 		69.1
(1.3)
P 		71.7
(1.6)
P 		47
B 82.1
(0.1)
P 		71.1
(-0.8)
P 		74.1
(-0.6)
P 		45
(-3)
P 		110.2
(2.4)
P 		117.1
(3)
P 		72
C 98.5
(0.6)
P 		76
(-1.1)
P 		54.2
(-2.8)
P 		51.9
(-3)
P 		82
(-0.7)
P 		129.6
(3)
P 		78
avr 72.2
(-0.8) 		76.5
(-0.3) 		67.7
(-1.3) 		52.6
(-3) 		87.1
(0.9) 		106.1
(3) 		54
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185272_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10577
Sequence Type Exemplar sequence
Representative Public ID K08D10.2 NCBI
Target Description K08D10.2 /REP_DB=WormBase Gene ID /WP=CE07354 /TR=Q21324 /GB=AAA98034.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068105(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068105 NCBI Caenorhabditis elegans DNaJ domain (prokaryotic heat shock protein) family member (dnj-15) (dnj-15) mRNA, complete cds. 11/11 None
SNAP00000009687 ENSEMBL cdna:SNAP chromosome:CEL140:IV:4176381:4179883:1 9/11 None
GENEFINDER00000009694 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:4176381:4178299:1 10/11 None
K08D10.2 ENSEMBL cdna:known chromosome:CEL140:IV:4176796:4178299:1 gene:K08D10.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:4177448-4178305(+) 96.12 96.12
Public Domain and Genome References
Gene Title
Gene Symbol dnj-15
Ensembl K08D10.2
Entrez Gene 187149 Entrez gene
SwissProt Q21324 EMBL-EBI
WormBase CE07354 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE68288 Hypothetical protein CBG13973 [Caenorhabditis briggsae] 6.0E-91
blast CAE68288 Hypothetical protein CBG13973 [Caenorhabditis briggsae] 2.0E-83
blast AAA98034 Dnaj domain (prokaryotic heat shock protein) protein 15 [Caenorhabditis elegans] ref|NP_500506.1| DNaJ domain (prokaryotic heat shock protein) family member (dnj-15) [Caenorhabditis elegans] 3.0E-78
blast AAA98034 Dnaj domain (prokaryotic heat shock protein) protein 15 [Caenorhabditis elegans] ref|NP_500506.1| DNaJ domain (prokaryotic heat shock protein) family member (dnj-15) [Caenorhabditis elegans] 1.0E-74
blast AAA98034 Dnaj domain (prokaryotic heat shock protein) protein 15 [Caenorhabditis elegans] ref|NP_500506.1| DNaJ domain (prokaryotic heat shock protein) family member (dnj-15) [Caenorhabditis elegans] 5.0E-67
blast CAE68288 Hypothetical protein CBG13973 [Caenorhabditis briggsae] 2.0E-57
blast CAH73876 OTTHUMP00000028547 [Homo sapiens] emb|CAI20339.1| OTTHUMP00000028547 [Homo sapiens] emb|CAG30348.1| dJ366L4.2 [Homo sapiens] gb|AAH65569.1| J-type co-chaperone HSC20 [Homo sapiens] ref|NP_741999.3| J-type co-chaperone HSC20 [Homo sapiens] gb|AAH00004.2| Unknown (protein for MGC:2637) [Homo sapiens] sp|Q8IWL3|HSC20_HUMAN Co-chaperone protein HscB, mitochondrial precursor (Hsc20) 8.0E-26
blast XP_341100 PREDICTED: similar to Co-chaperone protein HscB, mitochondrial precursor (Hsc20) [Rattus norvegicus] 4.0E-25
Protein Domains
Database ID Description E-Value
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 1.99999996755032E-17
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 1.00000000317108E-28
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 2.00000000634215E-29
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 1.5E-5
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 4.1E-10
Sequence
Target Sequence 
>C. ELEGANS:185272_AT
gtaaaatttcaccagctacaatccaaactgcatccggataaatttgtaatggcgactgac
gaggagaaaaagctctcagaagagcactcacggaagctcaacgaggcatataaagagctc
gccgacccatttaaacgtgcaaaatatttaataaaagaatacggcgaagaacatcccgaa
aatctgcaaaattccgaaattttgatggaaatgttggagagaaacgaggaaattgatgaa
atgcacgatgaaaatgcactgaaagctgaaaaatcgagaattgagacagaaatcgacgaa
caactcgagcaacttggcacacatttcgaatcgaaaaaaattccagaggctcgtgacgtc
atcggacgcttgacctacctgtattcgctcaaaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTAAAATTTCACCAGCTACAATCCA 486 461 25 Antisense
AGCTACAATCCAAACTGCATCCGGA 483 79 38 Antisense
TTTGTAATGGCGACTGACGAGGAGA 563 665 67 Antisense
AAGCTCTCAGAAGAGCACTCACGGA 694 149 94 Antisense
GCACTCACGGAAGCTCAACGAGGCA 337 311 108 Antisense
AACGAGGCATATAAAGAGCTCGCCG 532 141 124 Antisense
GCCGACCCATTTAAACGTGCAAAAT 368 283 145 Antisense
GGCGAAGAACATCCCGAAAATCTGC 560 539 187 Antisense
GACGAACAACTCGAGCAACTTGGCA 29 379 319 Antisense
GCAACTTGGCACACATTTCGAATCG 401 317 333 Antisense
TGACCTACCTGTATTCGCTCAAAAA 441 569 395 Antisense
Sequence Source Affymetrix Proprietary Database