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[185268_at]

Description W03F9.10 /REP_DB=WormBase Gene ID /WP=CE14540 /TR=O16997 /GB=AAG24205.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 74      6grp_high1_10_10 > 46      4grp_highest_8_8 > 34      4grp_high_8_8 > 57      6grp_highest_nonorm > 73      6grp_total > 46      4grp_total > 63      6grp_highest_sub > Cu      6grp_high1_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 337
1 2 3 4 range
A 772.6
(3)
P 		687.8
(-2)
P 		709.8
(-0.7)
P 		670.2
(-3)
P 		102
B 806.2
(3)
P 		579.8
(-2.2)
P 		737.7
(1.4)
P 		545.8
(-3)
P 		260
C 713
(2.5)
P 		575.5
(-0.6)
P 		735
(3)
P 		469.7
(-3)
P 		265
avr 763.9
(3) 		614.4
(-1.4) 		727.5
(1.9) 		561.9
(-3) 		202

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 312
1 2 3 4 5 6 range
A 606.9
(0)
P 		468.5
(-3)
P 		631.3
(0.6)
P 		536.2
(-1.5)
P 		504.4
(-2.2)
P 		743.4
(3)
P 		275
B 626.8
(1.5)
P 		676.6
(3)
P 		472.3
(-3)
P 		587.3
(0.4)
P 		511.5
(-1.8)
P 		599.9
(0.7)
P 		204
C 676.5
(3)
P 		676.1
(3)
P 		640.7
(2.1)
P 		431
(-3)
P 		452.4
(-2.5)
P 		621.5
(1.7)
P 		246
avr 636.7
(2.3) 		607.1
(1.3) 		581.4
(0.3) 		518.2
(-2) 		489.4
(-3) 		654.9
(3) 		166
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 202
1 2 3 4 range
A 281.3
(3)
P 		149.3
(-1.3)
P 		185.6
(-0.1)
P 		98.1
(-3)
P 		183
B 257.1
(3)
P 		143.2
(-1.9)
P 		170.9
(-0.7)
P 		116.3
(-3)
P 		141
C 236
(2.3)
P 		165.5
(-0.1)
P 		256.2
(3)
P 		79.2
(-3)
P 		177
avr 258.1
(3) 		152.7
(-0.9) 		204.2
(1) 		97.9
(-3) 		160

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 164
1 2 3 4 5 6 range
A 111.9
(-3)
P 		166.6
(1.7)
P 		137.5
(-0.8)
P 		136
(-0.9)
P 		152.2
(0.5)
P 		181
(3)
P 		69
B 218.1
(2.6)
P 		225.2
(3)
P 		114.7
(-3)
P 		155.6
(-0.8)
P 		207.8
(2.1)
P 		223.7
(2.9)
P 		111
C 250.7
(3)
P 		206.3
(1.4)
P 		163.1
(-0.2)
P 		86.7
(-3)
P 		146.4
(-0.8)
P 		208
(1.4)
P 		164
avr 193.6
(2.2) 		199.4
(2.6) 		138.4
(-2.1) 		126.1
(-3) 		168.8
(0.3) 		204.2
(3) 		78
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185268_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15318
Sequence Type Exemplar sequence
Representative Public ID W03F9.10 NCBI
Target Description W03F9.10 /REP_DB=WormBase Gene ID /WP=CE14540 /TR=O16997 /GB=AAG24205.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070740(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070740 NCBI Caenorhabditis elegans W03F9.10 (W03F9.10) mRNA, complete cds. 11/11 A 
SNAP00000001163 ENSEMBL cdna:SNAP chromosome:CEL140:V:128065:135068:1 11/11 None
GENEFINDER00000001180 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:128065:131375:1 11/11 None
W03F9.10.1 ENSEMBL cdna:known chromosome:CEL140:V:128065:131888:1 gene:W03F9.10 11/11 A 
W03F9.10.2 ENSEMBL cdna:known chromosome:CEL140:V:128065:131567:1 gene:W03F9.10 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:128064-131375(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl W03F9.10
Entrez Gene 178542 Entrez gene
SwissProt O16997 EMBL-EBI
WormBase CE14540 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:13639_AT proline-rich spliceosome-associated (PSP) family protein at
ATH1-121501:261872_S_AT pliceosome associated protein-related at
CANINE:1591633_AT similar to Splicing factor 3B subunit 2 (Spliceosome associated protein 145) (SAP 145) (SF3b150) (Pre-mRNA splicing factor SF3b 145 kDa subunit) cfa
CANINE:1590139_AT similar to Splicing factor 3B subunit 2 (Spliceosome associated protein 145) (SAP 145) (SF3b150) (Pre-mRNA splicing factor SF3b 145 kDa subunit) cfa
CANINE:1583619_AT similar to Splicing factor 3B subunit 2 (Spliceosome associated protein 145) (SAP 145) (SF3b150) (Pre-mRNA splicing factor SF3b 145 kDa subunit) cfa
CANINE_2:CFA.18141.1.S1_S_AT similar to Splicing factor 3B subunit 2 (Spliceosome associated protein 145) (SAP 145) (SF3b150) (Pre-mRNA splicing factor SF3b 145 kDa subunit) cfa
CANINE_2:CFA.3994.1.A1_AT similar to Splicing factor 3B subunit 2 (Spliceosome associated protein 145) (SAP 145) (SF3b150) (Pre-mRNA splicing factor SF3b 145 kDa subunit) cfa
CANINE_2:CFA.3994.1.A1_S_AT similar to Splicing factor 3B subunit 2 (Spliceosome associated protein 145) (SAP 145) (SF3b150) (Pre-mRNA splicing factor SF3b 145 kDa subunit) cfa
CANINE_2:CFA.6665.1.A1_S_AT similar to Splicing factor 3B subunit 2 (Spliceosome associated protein 145) (SAP 145) (SF3b150) (Pre-mRNA splicing factor SF3b 145 kDa subunit) cfa
DROSOPHILA_2:1638857_AT dm
DROSGENOME1:155098_AT dm
HG-U133A:200619_AT splicing factor 3b, subunit 2, 145kDa hs
HG-U133_PLUS_2:200619_AT splicing factor 3b, subunit 2, 145kDa hs
HG-FOCUS:200619_AT splicing factor 3b, subunit 2, 145kDa hs
HG-U133A_2:200619_AT splicing factor 3b, subunit 2, 145kDa hs
HUGENEFL:U41371_AT splicing factor 3b, subunit 2, 145kDa hs
U133_X3P:G5803154_3P_S_AT splicing factor 3b, subunit 2, 145kDa hs
U133_X3P:G5803154_3P_AT splicing factor 3b, subunit 2, 145kDa hs
U133_X3P:200619_3P_AT splicing factor 3b, subunit 2, 145kDa hs
HG-U133_PLUS_2:1558230_AT splicing factor 3b, subunit 2, 145kDa hs
HG-U95AV2:36973_AT splicing factor 3b, subunit 2, 145kDa hs
HG-U95C:61007_AT Splicing factor 3b, subunit 2, 145kDa hs
MOUSE430A_2:1438637_X_AT splicing factor 3b, subunit 2 mm
MOUSE430_2:1438637_X_AT splicing factor 3b, subunit 2 mm
MOE430A:1438637_X_AT splicing factor 3b, subunit 2 mm
MOUSE430_2:1459836_X_AT splicing factor 3b, subunit 2 mm
MOE430B:1459836_X_AT splicing factor 3b, subunit 2 mm
MU11KSUBA:AA268913_F_AT splicing factor 3b, subunit 2 mm
MG-U74BV2:164772_F_AT splicing factor 3b, subunit 2 mm
MG-U74CV2:170741_AT splicing factor 3b, subunit 2 mm
MG-U74CV2:169840_I_AT splicing factor 3b, subunit 2 mm
MOE430A:1433461_AT splicing factor 3b, subunit 2 mm
MOUSE430A_2:1433461_AT splicing factor 3b, subunit 2 mm
MOUSE430_2:1433461_AT splicing factor 3b, subunit 2 mm
MOE430A:1456352_A_AT splicing factor 3b, subunit 2 mm
MOUSE430A_2:1456352_A_AT splicing factor 3b, subunit 2 mm
MOUSE430_2:1456352_A_AT splicing factor 3b, subunit 2 mm
MOE430A:1456040_AT splicing factor 3b, subunit 2 mm
MOUSE430A_2:1456040_AT splicing factor 3b, subunit 2 mm
MOUSE430_2:1456040_AT splicing factor 3b, subunit 2 mm
MOE430B:1429362_A_AT splicing factor 3b, subunit 2 mm
MOUSE430_2:1429362_A_AT splicing factor 3b, subunit 2 mm
MOE430B:1453123_AT splicing factor 3b, subunit 2 mm
MOUSE430_2:1453123_AT splicing factor 3b, subunit 2 mm
MU19KSUBA:TC14259_AT Splicing factor 3b, subunit 2 (Sf3b2), mRNA mm
MU19KSUBB:TC32558_AT Splicing factor 3b, subunit 2 (Sf3b2), mRNA mm
RG-U34C:RC_AI178806_AT splicing factor 3b, subunit 2 (predicted) rn
RAE230A:1377070_AT splicing factor 3b, subunit 2 (predicted) rn
RAT230_2:1377070_AT splicing factor 3b, subunit 2 (predicted) rn
RAE230A:1388614_AT splicing factor 3b, subunit 2 (predicted) rn
RAT230_2:1388614_AT splicing factor 3b, subunit 2 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6397 mRNA processing inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_503141 W03F9.10 [Caenorhabditis elegans] gb|AAG24205.1| Hypothetical protein W03F9.10 [Caenorhabditis elegans] 0.0
blast CAE62369 Hypothetical protein CBG06453 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006568 EMBL-EBI PSP, proline-rich 1.5E-25
Pfam IPR007180 EMBL-EBI Protein of unknown function DUF382 1.3E-94
Sequence
Target Sequence 
>C. ELEGANS:185268_AT
gatcggatccgacgagagcagtgacgaggaagagtcggaggaggaagaacatgccgacga
tgatgatgctgatgttgaaggaggattccagacacctgcaccggttgagggaatgatcac
accatctggaatgacgactggaatcactggaatcgagactcctgacacgattgagctccg
gaaaggaaaagagagctctgtgctcggaacagacactcctgcggctgcctatcatatcat
tccggagaagaagaacgaacgtatcggtggacaaatgatggcatcctcccatacttatga
tctttccaagaagcatgcggttcgtgacgatggagttcagatttcgctggatcctgagtc
tattgatatggatgagcaaggactggcggctcggtacgaggagcagctccgaaagcaaaa
gcatcgggatgatgatgatgaggatcgagaggatttgacggatatggtggctgagcacgc
tgcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCGGATCCGACGAGAGCAGTGAC 559 417 1233 Antisense
GAAGGAGGATTCCAGACACCTGCAC 240 335 1309 Antisense
GACACCTGCACCGGTTGAGGGAATG 41 343 1323 Antisense
TGAGGGAATGATCACACCATCTGGA 594 567 1338 Antisense
GGAATCACTGGAATCGAGACTCCTG 86 533 1372 Antisense
GAGACTCCTGACACGATTGAGCTCC 510 393 1387 Antisense
GTGGACAAATGATGGCATCCTCCCA 209 491 1499 Antisense
GCATCCTCCCATACTTATGATCTTT 63 311 1513 Antisense
AGAAGCATGCGGTTCGTGACGATGG 43 75 1541 Antisense
GGAGTTCAGATTTCGCTGGATCCTG 574 517 1564 Antisense
GGATATGGTGGCTGAGCACGCTGCC 428 509 1692 Antisense
Sequence Source Affymetrix Proprietary Database