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[185234_s_at]

Description K02F3.10 /REP_DB=WormBase Gene ID /WP=CE01346 /TR=Q21154 /GB=AAK21418.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 24      6grp_high1_10_10 > 41      4grp_highest_8_8 > 25      4grp_high_8_8 > 60      6grp_highest_nonorm > 23      6grp_total > 41      4grp_total > 59      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1083
1 2 3 4 range
A 2180.6
(1.3)
P 		2061.2
(0.6)
P 		2465.6
(3)
P 		1462.4
(-3)
P 		1003
B 2116.9
(-1)
P 		2293.7
(0.7)
P 		2524.6
(3)
P 		1910.7
(-3)
P 		614
C 2350.7
(1.6)
P 		2380.3
(1.8)
P 		2545.6
(3)
P 		1736
(-3)
P 		810
avr 2216.1
(0.8) 		2245.1
(1) 		2511.9
(3) 		1703
(-3) 		809

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1398
1 2 3 4 5 6 range
A 1905.1
(-1.1)
P 		2319.9
(1.3)
P 		2617.4
(3)
P 		2263.9
(0.9)
P 		1710.5
(-2.3)
P 		1583.7
(-3)
P 		1034
B 2275.4
(-0.2)
P 		2314.2
(-0.1)
P 		2981.4
(3)
P 		2147.9
(-0.8)
P 		1867.6
(-2.1)
P 		1672.9
(-3)
P 		1309
C 2089.3
(-0.1)
P 		2371.4
(2.5)
P 		2422.3
(3)
P 		2283.6
(1.7)
P 		1879.9
(-2.1)
P 		1784.1
(-3)
P 		638
avr 2089.9
(-0.5) 		2335.2
(1) 		2673.7
(3) 		2231.8
(0.3) 		1819.3
(-2.2) 		1680.2
(-3) 		994
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 673
1 2 3 4 range
A 794
(3)
P 		447.4
(-0.6)
P 		644.6
(1.5)
P 		214.1
(-3)
P 		580
B 675.1
(3)
P 		566.5
(0.6)
P 		585
(1)
P 		407.2
(-3)
P 		268
C 778.1
(1.9)
P 		684.3
(1)
P 		887.2
(3)
P 		292.7
(-3)
P 		595
avr 749.1
(3) 		566.1
(0.5) 		705.6
(2.4) 		304.7
(-3) 		444

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 474
1 2 3 4 5 6 range
A 351.2
(-3)
P 		825.1
(3)
P 		570
(-0.2)
P 		574.2
(-0.2)
P 		516.1
(-0.9)
P 		385.6
(-2.6)
P 		474
B 791.7
(3)
P 		770.3
(2.4)
P 		724
(1.2)
P 		568.9
(-3)
P 		758.8
(2.1)
P 		623.8
(-1.5)
P 		223
C 774.4
(3)
P 		723.6
(2)
P 		616.5
(-0)
P 		459.2
(-3)
P 		608.3
(-0.2)
P 		597.2
(-0.4)
P 		315
avr 639.1
(-0.4) 		773
(3) 		636.8
(-0.4) 		534.1
(-3) 		627.7
(-0.6) 		535.5
(-3) 		239
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 185234_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8332
Sequence Type Exemplar sequence
Representative Public ID K02F3.10 NCBI
Target Description K02F3.10 /REP_DB=WormBase Gene ID /WP=CE01346 /TR=Q21154 /GB=AAK21418.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064874(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064874 NCBI Caenorhabditis elegans K02F3.10 (K02F3.10) mRNA, complete cds. 11/11 None
SNAP00000008341 ENSEMBL cdna:SNAP chromosome:CEL140:III:851434:852384:-1 11/11 None
GENEFINDER00000008348 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:851434:852384:-1 11/11 None
K02F3.10 ENSEMBL cdna:known chromosome:CEL140:III:851387:852384:-1 gene:K02F3.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:851433-852384(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K02F3.10
Entrez Gene 175243 Entrez gene
SwissProt Q21154 EMBL-EBI
WormBase CE01346 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6418 tRNA aminoacylation for protein translation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4812 tRNA ligase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK21418 Hypothetical protein K02F3.10 [Caenorhabditis elegans] ref|NP_497275.1| K02F3.10 [Caenorhabditis elegans] 1.0E-112
blast CAE56474 Hypothetical protein CBG24188 [Caenorhabditis briggsae] 2.0E-97
blast NP_001030203 hypothetical protein LOC506138 [Bos taurus] gb|AAI03215.1| Unknown (protein for MGC:128446) [Bos taurus] 3.0E-9
Sequence
Target Sequence 
>C. ELEGANS:185234_S_AT
tggagacaatttcaaacgccggtgaacaagtgacaaatgtttttggacaattctggcaac
ttgtcacctcgaaaaacacgacgaataatggagattcaaaacctattaagattgagcaac
ttccgatttatgctgaagataatgctccactgaagcagaaattccttccagaagagccac
ttcctcttcaaagagaattcgcgactattcgtatcgcatgtgagcaggagtatgatcgtg
ttgctgaacgcttcaaagttgtcgattgtgcgatgactcaaacaaaaaaagcggcgacca
aatgcaatgcatatctgacagaagaatggactgctcttccaaaagccgcagcaatcaccg
ttggtggaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGAGACAATTTCAAACGCCGGTGA 155 547 35 Antisense
TTCTGGCAACTTGTCACCTCGAAAA 355 693 85 Antisense
AAGATTGAGCAACTTCCGATTTATG 653 155 142 Antisense
ATTCCTTCCAGAAGAGCCACTTCCT 623 7 195 Antisense
AGAGAATTCGCGACTATTCGTATCG 245 69 226 Antisense
ATTCGTATCGCATGTGAGCAGGAGT 395 7 241 Antisense
GAACGCTTCAAAGTTGTCGATTGTG 470 347 280 Antisense
TGTCGATTGTGCGATGACTCAAACA 225 557 294 Antisense
AAAGCGGCGACCAAATGCAATGCAT 359 125 322 Antisense
TGGACTGCTCTTCCAAAAGCCGCAG 167 549 361 Antisense
GCCGCAGCAATCACCGTTGGTGGAA 176 285 379 Antisense
Sequence Source Affymetrix Proprietary Database