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[185233_at]

Description K08D10.7 /REP_DB=WormBase Gene ID /WP=CE07359 /TR=Q21319 /GB=AAA98029.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 96      4grp_total > 32      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 80
1 2 3 4 range
A 56.8
(1.7)
P 		23.7
(-3)
A 		37.8
(-1)
A 		66
(3)
A 		42
B 25.3
(-3)
A 		28.5
(-2.7)
A 		90
(3)
A 		63.5
(0.5)
A 		65
C 9.9
(-3)
A 		34.5
(3)
A 		27.2
(1.2)
A 		21.5
(-0.2)
A 		25
avr 30.7
(-2.5) 		28.9
(-3) 		51.7
(3) 		50.3
(2.6) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 86
1 2 3 4 5 6 range
A 13.2
(-3)
A 		22.7
(-2.3)
A 		44.6
(-0.7)
A 		65.3
(0.8)
A 		66.7
(0.9)
A 		94.7
(3)
P 		82
B 49
(0.4)
A 		8.6
(-3)
A 		28.4
(-1.3)
A 		19.6
(-2.1)
A 		80.1
(3)
A 		58.5
(1.2)
P 		72
C 33.7
(-2.1)
A 		24.1
(-3)
A 		54
(-0.2)
A 		49.8
(-0.6)
A 		67.6
(1.1)
A 		87.6
(3)
P 		64
avr 32
(-1.7) 		18.5
(-3) 		42.3
(-0.7) 		44.9
(-0.4) 		71.5
(2.1) 		80.3
(3) 		62
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 20.7
(3)
P 		5.1
(-3)
A 		9.9
(-1.2)
A 		9.7
(-1.2)
A 		16
B 8.1
(-2.5)
A 		7
(-3)
A 		20.8
(3)
A 		13.5
(-0.2)
A 		14
C 3.3
(-3)
A 		9.9
(3)
A 		9.5
(2.6)
A 		3.6
(-2.7)
A 		7
avr 10.7
(0.3) 		7.3
(-3) 		13.4
(3) 		8.9
(-1.4) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 30
1 2 3 4 5 6 range
A 2.4
(-3)
A 		8.1
(-1.3)
A 		9.7
(-0.9)
A 		16.6
(1.1)
A 		20.1
(2.1)
A 		23.1
(3)
P 		21
B 17
(-0.1)
A 		2.9
(-3)
A 		6.9
(-2.2)
A 		5.2
(-2.5)
A 		32.5
(3)
A 		21.8
(0.8)
P 		30
C 12.5
(-1.6)
A 		7.3
(-3)
A 		13.7
(-1.3)
A 		10
(-2.3)
A 		21.9
(1)
A 		29.3
(3)
P 		22
avr 10.6
(-1.6) 		6.1
(-3) 		10.1
(-1.7) 		10.6
(-1.6) 		24.8
(3) 		24.7
(3) 		19
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185233_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10618
Sequence Type Exemplar sequence
Representative Public ID K08D10.7 NCBI
Target Description K08D10.7 /REP_DB=WormBase Gene ID /WP=CE07359 /TR=Q21319 /GB=AAA98029.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068100(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068100 NCBI Caenorhabditis elegans K08D10.7 (K08D10.7) mRNA, complete cds. 11/11 None
SNAP00000009682 ENSEMBL cdna:SNAP chromosome:CEL140:IV:4164772:4166169:1 11/11 None
GENEFINDER00000009699 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:4164772:4166169:1 11/11 None
K08D10.7 ENSEMBL cdna:known chromosome:CEL140:IV:4164772:4166169:1 gene:K08D10.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:4164777-4166175(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K08D10.7
Entrez Gene 187152 Entrez gene
SwissProt Q21319 EMBL-EBI
WormBase CE07359 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA98029 Hypothetical protein K08D10.7 [Caenorhabditis elegans] ref|NP_500501.1| K08D10.7 [Caenorhabditis elegans] 1.0E-169
blast AAA98028 Hypothetical protein K08D10.8 [Caenorhabditis elegans] ref|NP_500500.1| K08D10.8 [Caenorhabditis elegans] 1.0E-150
blast CAB04311 Hypothetical protein F46A8.10 [Caenorhabditis elegans] ref|NP_492890.2| F46A8.10 [Caenorhabditis elegans] 1.0E-109
Protein Domains
Database ID Description E-Value
Pfam IPR005552 EMBL-EBI Scramblase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:185233_AT
gactccaaatcgctatgtggtccacgacatgtactgtcgtccgctgctttattgtatgga
aagatcaaacgtctttgctcgtcagtaccacggaaacaatcgtaacttcggaatgcagat
catggatacgcacggagcccaggtgatgacttgctttcgaggaagaccatgctgctcatg
cgatgatttcctgagcacccagttcctcgatcagcagattgggatgatgaagagggagtg
ctgtaatcctaacttcgagctcatcggatccggctgcaaccagcccctgctcatccaatc
tccagtttgtgctggctgtggtggtactcagaaatttccagttatgacctacaatggcgc
actcgtcggagaaatcgtccgcctctacccgggattcatgcaggagatgttcaccgatgc
ggacacctatattgttcattttccaatggacatgccgccaatcttgaagcttctgctcgt
cacctcggttttcttgatcgacttcacctactttgaggatcgtaaccaggatcgtcatca
tcatggtggg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACTCCAAATCGCTATGTGGTCCAC 381 373 339 Antisense
CACGACATGTACTGTCGTCCGCTGC 257 199 361 Antisense
GGAGCCCAGGTGATGACTTGCTTTC 136 523 472 Antisense
AAGACCATGCTGCTCATGCGATGAT 199 141 501 Antisense
CATGCGATGATTTCCTGAGCACCCA 280 211 515 Antisense
GTAATCCTAACTTCGAGCTCATCGG 251 463 581 Antisense
TGTGCTGGCTGTGGTGGTACTCAGA 433 557 646 Antisense
TATGACCTACAATGGCGCACTCGTC 236 653 681 Antisense
GGCGCACTCGTCGGAGAAATCGTCC 346 541 694 Antisense
TCGTCACCTCGGTTTTCTTGATCGA 242 605 815 Antisense
CCAGGATCGTCATCATCATGGTGGG 118 273 864 Antisense
Sequence Source Affymetrix Proprietary Database