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[185223_at]

Description K02F3.12 /REP_DB=WormBase Gene ID /WP=CE26682 /TR=Q9TXJ8 /GB=AAK21428.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 90      4grp_total > 54      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 318
1 2 3 4 range
A 322.7
(2.8)
P 		330.5
(3)
A 		166.4
(-1)
A 		83.4
(-3)
A 		247
B 401.8
(3)
P 		188.5
(-2.7)
A 		281.2
(-0.2)
A 		177.8
(-3)
A 		224
C 303.4
(3)
P 		171.6
(-2)
A 		197.6
(-1)
P 		145.9
(-3)
A 		158
avr 342.6
(3) 		230.2
(-0.3) 		215.1
(-0.7) 		135.7
(-3) 		207

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 210
1 2 3 4 5 6 range
A 130
(-2.3)
A 		314
(3)
P 		104.3
(-3)
P 		271.2
(1.8)
P 		127.3
(-2.3)
A 		287
(2.2)
A 		210
B 305.5
(3)
P 		239.5
(0.8)
P 		196.8
(-0.6)
P 		268.2
(1.8)
P 		168
(-1.5)
A 		122.3
(-3)
P 		183
C 213.4
(-0.4)
P 		269.6
(1.8)
P 		260.7
(1.4)
P 		301.9
(3)
A 		275.3
(2)
A 		145.4
(-3)
P 		157
avr 216.3
(-1) 		274.4
(2.6) 		187.3
(-2.8) 		280.4
(3) 		190.2
(-2.7) 		184.9
(-3) 		96
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 116
1 2 3 4 range
A 117.5
(3)
P 		71.7
(0.4)
A 		43.5
(-1.2)
A 		12.2
(-3)
A 		105
B 128.1
(3)
P 		46.6
(-2.4)
A 		65.2
(-1.2)
A 		37.9
(-3)
A 		90
C 100.4
(3)
P 		49.3
(-1)
A 		68.9
(0.5)
P 		24.6
(-3)
A 		76
avr 115.3
(3) 		55.9
(-0.9) 		59.2
(-0.7) 		24.9
(-3) 		90

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 89
1 2 3 4 5 6 range
A 24
(-2.9)
A 		111.7
(3)
P 		22.7
(-3)
P 		68.8
(0.1)
P 		38.4
(-1.9)
A 		69.9
(0.2)
A 		89
B 106.3
(3)
P 		79.7
(0.4)
P 		47.8
(-2.8)
P 		71
(-0.5)
P 		68.3
(-0.8)
A 		45.6
(-3)
P 		61
C 79.1
(1.5)
P 		82.3
(2)
P 		66.4
(-0.4)
P 		60.7
(-1.2)
A 		89.1
(3)
A 		48.7
(-3)
P 		40
avr 69.8
(0.2) 		91.2
(3) 		45.6
(-3) 		66.8
(-0.2) 		65.3
(-0.4) 		54.7
(-1.8) 		46
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185223_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8318
Sequence Type Exemplar sequence
Representative Public ID K02F3.12 NCBI
Target Description K02F3.12 /REP_DB=WormBase Gene ID /WP=CE26682 /TR=Q9TXJ8 /GB=AAK21428.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027485(11), NM_001027486(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027485 NCBI Caenorhabditis elegans K02F3.12a (K02F3.12) mRNA, complete cds. 11/11 None
NM_001027486 NCBI Caenorhabditis elegans K02F3.12b (K02F3.12) mRNA, complete cds. 11/11 None
K02F3.12a ENSEMBL cdna:known chromosome:CEL140:III:856563:860062:1 gene:K02F3.12 11/11 None
K02F3.12b ENSEMBL cdna:known chromosome:CEL140:III:856627:860036:1 gene:K02F3.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:856562-858548(+) 97.84 100.0
Public Domain and Genome References
Ensembl K02F3.12
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK21428 Hypothetical protein K02F3.12a [Caenorhabditis elegans] ref|NP_001022656.1| K02F3.12a [Caenorhabditis elegans] 0.0
blast AAW88393 Hypothetical protein K02F3.12b [Caenorhabditis elegans] ref|NP_001022657.1| K02F3.12b [Caenorhabditis elegans] 0.0
blast P46064 Putative ATP-dependent DNA helicase Q1 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 5.7E-26
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 4.9E-39
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 5.7E-26
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 4.9E-39
Sequence
Target Sequence 
>C. ELEGANS:185223_AT
atatgctcgggatccaagcagctctcacattccgtgccggattcaacagatcaaatttga
agtataaagtggttcaaaaaccaggatccgaagacgagtgtaccgaggaaatcgcaaaga
ccatcaaaagagattttgccggacaaactggcatcatttactgcctatcacgaaatgatt
gtgaaaaagtggcaaaagcgttgaaatcgcatggaatcaaggcaaaacactatcatgcct
atatggaacctgtcgatcgaagtggagctcatcagggttggatttccggaaaaattcaag
tgattgtggcgactgtcgcattcggaatgggcattgataagccaaatgaaagtggaagag
caggtagagatggacagcctgccacgtgtattctatattatcgactcgcagatatcttta
aacaatcgtcaatggtacaacaagaacgaacgggtattcagaatttgtataatatggtta
gatatgcggctgattcgagcacatgtcgacgggtaaagcttgcggagca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATATGCTCGGGATCCAAGCAGCTCT 535 21 866 Antisense
CTCTCACATTCCGTGCCGGATTCAA 376 231 887 Antisense
GGATCCGAAGACGAGTGTACCGAGG 324 511 949 Antisense
ACTGGCATCATTTACTGCCTATCAC 310 97 1012 Antisense
ATGCCTATATGGAACCTGTCGATCG 516 39 1100 Antisense
GAGCTCATCAGGGTTGGATTTCCGG 124 389 1130 Antisense
TGGCGACTGTCGCATTCGGAATGGG 620 543 1172 Antisense
TAGAGATGGACAGCCTGCCACGTGT 576 651 1230 Antisense
AGCCTGCCACGTGTATTCTATATTA 182 83 1241 Antisense
GATATGCGGCTGATTCGAGCACATG 405 425 1346 Antisense
GTCGACGGGTAAAGCTTGCGGAGCA 15 473 1370 Antisense
Sequence Source Affymetrix Proprietary Database