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[185221_at]

Description Y49F6B.1 /REP_DB=WormBase Gene ID /WP=CE25333 /TR=Q9N4U0 /GB=AAF60728.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 47      4grp_total > 38      6grp_cgc > 07      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 116
1 2 3 4 range
A 121.4
(3)
A 		14.5
(-3)
A 		20.5
(-2.7)
A 		15
(-3)
A 		107
B 106.8
(3)
A 		49.3
(-0.4)
A 		14.2
(-2.5)
A 		5.7
(-3)
A 		101
C 11.4
(-3)
A 		29.6
(-1.6)
A 		19.2
(-2.4)
A 		87.6
(3)
A 		76
avr 79.9
(3) 		31.1
(-1.7) 		18
(-3) 		36.1
(-1.2) 		62

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 81
1 2 3 4 5 6 range
A 22.5
(0.6)
A 		13.4
(-1.1)
A 		34.6
(3)
A 		10.4
(-1.7)
A 		3.9
(-3)
A 		11.5
(-1.5)
A 		31
B 84.2
(3)
A 		9
(-2.6)
A 		15.5
(-2.1)
A 		5.8
(-2.8)
A 		3.2
(-3)
A 		12.8
(-2.3)
A 		81
C 4.6
(-2.9)
A 		5.4
(-2.9)
A 		4.1
(-3)
A 		15.8
(-1.7)
A 		9.7
(-2.4)
A 		56.6
(3)
A 		53
avr 37.1
(3) 		9.3
(-2.3) 		18.1
(-0.6) 		10.7
(-2) 		5.6
(-3) 		27
(1.1) 		32
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 43
1 2 3 4 range
A 44.2
(3)
A 		3.1
(-2.9)
A 		5.4
(-2.5)
A 		2.2
(-3)
A 		42
B 34.1
(3)
A 		12.2
(-1)
A 		3.3
(-2.6)
A 		1.2
(-3)
A 		33
C 3.8
(-3)
A 		8.5
(-0.4)
A 		6.7
(-1.4)
A 		14.8
(3)
A 		11
avr 27.4
(3) 		7.9
(-2.2) 		5.1
(-3) 		6.1
(-2.7) 		22

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 28
1 2 3 4 5 6 range
A 4.1
(-0.2)
A 		4.8
(0.4)
A 		7.5
(3)
A 		2.6
(-1.7)
A 		1.2
(-3)
A 		2.8
(-1.5)
A 		6
B 29.3
(3)
A 		3
(-2.6)
A 		3.8
(-2.5)
A 		1.5
(-3)
A 		1.3
(-3)
A 		4.8
(-2.3)
A 		28
C 1.7
(-2.8)
A 		1.7
(-2.8)
A 		1
(-3)
A 		3.2
(-2.3)
A 		3.1
(-2.3)
A 		19
(3)
A 		18
avr 11.7
(3) 		3.2
(-2.2) 		4.1
(-1.7) 		2.4
(-2.7) 		1.9
(-3) 		8.9
(1.3) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185221_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4407
Sequence Type Exemplar sequence
Representative Public ID Y49F6B.1 NCBI
Target Description Y49F6B.1 /REP_DB=WormBase Gene ID /WP=CE25333 /TR=Q9N4U0 /GB=AAF60728.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062163(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062163 NCBI Caenorhabditis elegans CYclin H family member (cyh-1) (cyh-1) mRNA, complete cds. 11/11 None
SNAP00000021102 ENSEMBL cdna:SNAP chromosome:CEL140:II:3505014:3512820:1 11/11 None
GENEFINDER00000021118 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3505014:3509444:1 11/11 None
Y49F6B.1 ENSEMBL cdna:known chromosome:CEL140:II:3505012:3509609:1 gene:Y49F6B.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3505564-3509443(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol cyh-1
Ensembl Y49F6B.1
Entrez Gene 190078 Entrez gene
SwissProt Q9N4U0 EMBL-EBI
WormBase CE36037 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:246762_AT cyclin family protein at
CANINE:1601936_AT similar to Cyclin H (MO15-associated protein) (p37) (p34) cfa
CANINE_2:CFAAFFX.13291.1.S1_AT similar to Cyclin H (MO15-associated protein) (p37) (p34) cfa
CANINE_2:CFAAFFX.13291.1.S1_S_AT similar to Cyclin H (MO15-associated protein) (p37) (p34) cfa
DROSOPHILA_2:1640346_AT Cyclin H dm
DROSGENOME1:142767_AT Cyclin H dm
CHICKEN:GGA.11839.1.S1_S_AT cyclin H gga
CHICKEN:GGA.9802.1.S1_AT cyclin H gga
HG-U95AV2:1924_AT cyclin H hs
HC-G110:1924_AT cyclin H hs
HUGENEFL:U11791_AT cyclin H hs
HG-U133A:204093_AT cyclin H hs
HG-U133_PLUS_2:204093_AT cyclin H hs
HG-FOCUS:204093_AT cyclin H hs
HG-U133A_2:204093_AT cyclin H hs
U133_X3P:G4502622_3P_S_AT cyclin H hs
U133_X3P:G4502622_3P_AT cyclin H hs
U133_X3P:204093_3P_AT cyclin H hs
HG-U95AV2:36086_AT cyclin H hs
HG-U95E:71416_AT cyclin H hs
HG-U133_PLUS_2:238243_AT Cyclin H hs
HG-U133B:238243_AT Cyclin H hs
HG-U95E:71558_AT Cyclin H hs
HG-U95D:77853_R_AT Cyclin H hs
HU35KSUBC:RC_N22145_AT Cyclin H hs
U133_X3P:HS.185268.0.A1_3P_AT Cyclin H hs
MOUSE430_2:1456293_S_AT cyclin H mm
MOE430A:1456293_S_AT cyclin H mm
MOUSE430A_2:1456293_S_AT cyclin H mm
MU11KSUBB:MSA.31187.0_S_AT cyclin H mm
MG-U74BV2:116360_AT cyclin H mm
MG-U74BV2:162561_AT cyclin H mm
MOE430A:1418584_AT cyclin H mm
MOUSE430A_2:1418584_AT cyclin H mm
MOUSE430_2:1418584_AT cyclin H mm
MOE430A:1418585_AT cyclin H mm
MOUSE430A_2:1418585_AT cyclin H mm
MOUSE430_2:1418585_AT cyclin H mm
MG-U74BV2:116703_AT Cyclin H, mRNA (cDNA clone MGC:47913 IMAGE:1446473) mm
MG-U74CV2:135537_AT Cyclin H, mRNA (cDNA clone MGC:47913 IMAGE:1446473) mm
MOE430B:1438998_AT Cyclin H, mRNA (cDNA clone MGC:47913 IMAGE:1446473) mm
MOUSE430_2:1438998_AT Cyclin H, mRNA (cDNA clone MGC:47913 IMAGE:1446473) mm
MU19KSUBC:TC41387_AT Cyclin H, mRNA (cDNA clone MGC:47913 IMAGE:1446473) mm
RG-U34C:RC_AI177687_AT cyclin H rn
RG-U34C:RC_AI111344_AT cyclin H rn
RAE230A:1368083_AT cyclin H rn
RAT230_2:1368083_AT cyclin H rn
RAE230B:1379535_AT Cyclin H rn
RAT230_2:1379535_AT Cyclin H rn
RAE230B:1382842_AT Cyclin H rn
RAT230_2:1382842_AT Cyclin H rn
RG-U34B:RC_AI043751_AT Cyclin H rn
RG-U34C:RC_AI073064_AT Cyclin H rn
RG-U34C:RC_AI137532_AT Cyclin H rn
YEAST_2:1771776_AT Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters Sc
Protein Similarities
Method ID Description E-Value
blast NP_494564 CYclin H family member (cyh-1) [Caenorhabditis elegans] gb|AAF60728.2| Cyclin h protein 1 [Caenorhabditis elegans] 0.0
blast CAE70953 Hypothetical protein CBG17764 [Caenorhabditis briggsae] 1.0E-147
blast CAE70953 Hypothetical protein CBG17764 [Caenorhabditis briggsae] 1.0E-143
blast EAL31018 GA20328-PA [Drosophila pseudoobscura] 3.0E-32
Sequence
Target Sequence 
>C. ELEGANS:185221_AT
ttgactgtacattgcccgtataggccatacgaaggccatctaatggatatgaagacccga
atgcttcttctaaatttcgatctcgaatcaatccgccgcgattctatgagattcttccag
aatgcactacaaaccgacgtgcttctcctctatccaccgtcacagattgcactggccgcc
attaatttcgggctccatgctcagggcaaatccgacgagattctacgcgagtttcttcgg
aaacttatcggaatcgaagaggactcgtgggctcatcgtgatgctcgtccagaggatttg
gaaaagctcgaaaagacggttacgagagtcaatcaaataatgcgagaagtcgatcgaaat
ttcgtagcagtgacagagcaagaacgggagaatcacaaaagccagctggcacggattcaa
gcattgattccagcacttgatgaacgacgaaaagagcaatggttggctggtggaggtgtt
ggattgatgcctggagagctcgcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGACTGTACATTGCCCGTATAGGC 108 703 364 Antisense
ATGCTTCTTCTAAATTTCGATCTCG 405 43 424 Antisense
ATCAATCCGCCGCGATTCTATGAGA 303 27 450 Antisense
GCACTACAAACCGACGTGCTTCTCC 379 315 487 Antisense
ATCCACCGTCACAGATTGCACTGGC 244 41 515 Antisense
GCACTGGCCGCCATTAATTTCGGGC 487 313 532 Antisense
GCTCCATGCTCAGGGCAAATCCGAC 58 297 555 Antisense
GACGAGATTCTACGCGAGTTTCTTC 556 379 577 Antisense
CGAAGAGGACTCGTGGGCTCATCGT 582 253 618 Antisense
AAGCCAGCTGGCACGGATTCAAGCA 432 149 762 Antisense
TGGATTGATGCCTGGAGAGCTCGCC 319 551 843 Antisense
Sequence Source Affymetrix Proprietary Database