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[185220_s_at]

Description D2096.11 /REP_DB=WormBase Gene ID /WP=CE04309 /TR=Q19011 /GB=AAA81498.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 87      6grp_total > 77      4grp_total > 31      6grp_high1_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 140
1 2 3 4 range
A 229.7
(3)
P 		125.5
(-3)
A 		160.5
(-1)
A 		169.1
(-0.5)
P 		104
B 209
(3)
P 		109.6
(-2.1)
A 		91.7
(-3)
A 		182.3
(1.6)
P 		117
C 231.2
(3)
P 		111.6
(-3)
A 		174
(0.1)
A 		163.9
(-0.4)
P 		120
avr 223.3
(3) 		115.6
(-3) 		142.1
(-1.5) 		171.8
(0.1) 		108

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 508
1 2 3 4 5 6 range
A 128
(-2.8)
P 		125.4
(-2.9)
P 		125.7
(-2.9)
P 		121.6
(-3)
A 		285.1
(1.1)
P 		361.9
(3)
P 		240
B 115
(-3)
P 		157.5
(-2.5)
A 		135.5
(-2.7)
P 		232
(-1.5)
P 		177.6
(-2.2)
P 		594.7
(3)
P 		480
C 86.4
(-3)
M 		122.5
(-2.6)
P 		116.7
(-2.6)
P 		122.2
(-2.6)
M 		193.7
(-1.7)
P 		587
(3)
P 		501
avr 109.8
(-3) 		135.1
(-2.6) 		126
(-2.8) 		158.6
(-2.3) 		218.8
(-1.4) 		514.5
(3) 		405
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 62
1 2 3 4 range
A 83.6
(3)
P 		27.2
(-2.7)
A 		42
(-1.2)
A 		24.7
(-3)
P 		59
B 66.7
(3)
P 		27.1
(-2.2)
A 		21.3
(-3)
A 		38.9
(-0.7)
P 		45
C 76.5
(3)
P 		32.1
(-2.4)
A 		60.6
(1)
A 		27.6
(-3)
P 		49
avr 75.6
(3) 		28.8
(-3) 		41.3
(-1.4) 		30.4
(-2.8) 		47

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 198
1 2 3 4 5 6 range
A 23.6
(-3)
P 		44.6
(-1)
P 		27.4
(-2.6)
P 		30.8
(-2.3)
A 		86
(2.8)
P 		88.1
(3)
P 		65
B 40
(-2.8)
P 		52.4
(-2.4)
A 		32.9
(-3)
P 		61.5
(-2.1)
P 		72.2
(-1.8)
P 		221.7
(3)
P 		189
C 32
(-2.7)
M 		37.4
(-2.6)
P 		29.7
(-2.8)
P 		24.6
(-3)
M 		62.7
(-1.7)
P 		196.5
(3)
P 		172
avr 31.9
(-2.9) 		44.8
(-2.4) 		30
(-3) 		39
(-2.6) 		73.6
(-1.1) 		168.8
(3) 		139
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 185220_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10029
Sequence Type Exemplar sequence
Representative Public ID D2096.11 NCBI
Target Description D2096.11 /REP_DB=WormBase Gene ID /WP=CE04309 /TR=Q19011 /GB=AAA81498.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069025(11), NM_069031(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069025 NCBI Caenorhabditis elegans D2096.11 (D2096.11) mRNA, complete cds. 11/11 None
NM_069031 NCBI Caenorhabditis elegans M116.5 (M116.5) mRNA, complete cds. 11/11 A 
SNAP00000025677 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8387899:8393844:-1 11/11 None
GENEFINDER00000025699 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8388661:8393880:-1 11/11 None
D2096.11 ENSEMBL cdna:known chromosome:CEL140:IV:8389129:8392845:-1 gene:D2096.11 11/11 None
M116.5 ENSEMBL cdna:known chromosome:CEL140:IV:8414275:8420990:-1 gene:M116.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8389134-8394189(-) 99.18 100.0
chrIV:8414914-8418802(-) 86.86 87.67
Public Domain and Genome References
Gene Title

Ensembl D2096.11
M116.5
Entrez Gene 177638 Entrez gene
177642 Entrez gene
SwissProt Q19011 EMBL-EBI
Q965L0 EMBL-EBI
WormBase CE33608 Wormbase
CE35345 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7050 cell cycle arrest inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3779 actin binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_501426 D2096.11 [Caenorhabditis elegans] gb|AAA81498.4| Hypothetical protein D2096.11 [Caenorhabditis elegans] 0.0
blast AAK71387 Hypothetical protein M116.5 [Caenorhabditis elegans] ref|NP_501432.2| M116.5 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
scop a.4.1.Centromere-binding All alpha proteins; DNA/RNA-binding 3-helical bundle; Homeodomain-like; Centromere-binding 2.20000004768372
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 1.6E-6
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 1.4E-11
Pfam IPR003108 EMBL-EBI Growth-arrest-specific protein 2 2.3E-34
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 2.9E-31
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 4.8E-31
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 4.5E-7
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 1.4E-11
Pfam IPR003108 EMBL-EBI Growth-arrest-specific protein 2 1.1E-23
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 5.0E-12
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 1.6E-6
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 1.4E-11
Pfam IPR003108 EMBL-EBI Growth-arrest-specific protein 2 2.3E-34
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 5.0E-12
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 1.6E-6
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 1.4E-11
Pfam IPR003108 EMBL-EBI Growth-arrest-specific protein 2 2.3E-34
Sequence
Target Sequence 
>C. ELEGANS:185220_S_AT
ggttcgtatccttcgatcaactgtgatggttcgtgttggcggtggatgggagccacttga
agagtcacttcaaaaacatgatccatgccgtacaaaaggacgtctcaatatcaatatgtt
cccagaagctcgtccaattcatgctcttgacttgatgcaatcgttcactaagaatcggca
tgccaaacagatgcccattgccggaacgcctggaccaattatgaagattcgcgaaaaaac
tgagagaagtgttcccatgagcggaggactcggaggaaccgctggttacacagttacaac
tgaatcgcctagaaaaacgcctcaacgcccgtttcgaattccacgtactccatcggacaa
gacccctggacgccaaagccgtgttggat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTTCGTATCCTTCGATCAACTGTG 197 507 2532 Antisense
ATGGTTCGTGTTGGCGGTGGATGGG 339 51 2557 Antisense
TGGGAGCCACTTGAAGAGTCACTTC 176 553 2578 Antisense
TCGTCCAATTCATGCTCTTGACTTG 334 605 2661 Antisense
GTTCACTAAGAATCGGCATGCCAAA 98 445 2694 Antisense
GCATGCCAAACAGATGCCCATTGCC 18 309 2709 Antisense
GTGTTCCCATGAGCGGAGGACTCGG 459 485 2780 Antisense
GGAACCGCTGGTTACACAGTTACAA 702 529 2806 Antisense
CAGTTACAACTGAATCGCCTAGAAA 225 207 2822 Antisense
TCCACGTACTCCATCGGACAAGACC 405 587 2871 Antisense
CCTGGACGCCAAAGCCGTGTTGGAT 99 267 2896 Antisense
Sequence Source Affymetrix Proprietary Database