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[185217_at]

Description C32B5.15 /REP_DB=WormBase Gene ID /WP=CE08523 /TR=P91114 /GB=AAB37970.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 36      4grp_total > 28      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 150
1 2 3 4 range
A 101
(-0.1)
P 		80.5
(-1.2)
A 		46
(-3)
A 		161.7
(3)
P 		116
B 100.6
(3)
P 		71
(1)
A 		11.6
(-3)
A 		83.8
(1.9)
M 		89
C 66.3
(0.7)
A 		90.5
(3)
A 		27.1
(-3)
A 		77
(1.7)
P 		63
avr 89.3
(1.6) 		80.7
(1) 		28.2
(-3) 		107.5
(3) 		79

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 79
1 2 3 4 5 6 range
A 78
(3)
A 		46.7
(0.1)
P 		49.3
(0.3)
A 		41.1
(-0.4)
A 		13.1
(-3)
A 		64.4
(1.7)
A 		65
B 82.6
(3)
P 		72.1
(1.9)
P 		25.1
(-3)
A 		33.2
(-2.2)
A 		35.1
(-2)
A 		57.6
(0.4)
A 		58
C 41.1
(-1.5)
A 		71.4
(1.2)
A 		34.3
(-2.1)
A 		91.8
(3)
A 		24.1
(-3)
A 		59
(0.1)
A 		68
avr 67.2
(3) 		63.4
(2.5) 		36.2
(-1.3) 		55.4
(1.4) 		24.1
(-3) 		60.3
(2) 		43
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 34
1 2 3 4 range
A 36.8
(3)
P 		17.5
(-1.7)
A 		12
(-3)
A 		23.7
(-0.2)
P 		25
B 32.1
(3)
P 		17.5
(0)
A 		2.7
(-3)
A 		17.8
(0.1)
M 		29
C 21.9
(1.5)
A 		26
(3)
A 		9.4
(-3)
A 		13
(-1.7)
P 		17
avr 30.3
(3) 		20.3
(0.3) 		8
(-3) 		18.2
(-0.3) 		22

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 25
1 2 3 4 5 6 range
A 14.4
(2)
A 		16.6
(3)
P 		10.7
(0.2)
A 		10.4
(0)
A 		4
(-3)
A 		15.7
(2.6)
A 		13
B 28.7
(3)
P 		24
(1.8)
P 		6.1
(-3)
A 		8.8
(-2.3)
A 		14.3
(-0.8)
A 		21.5
(1.1)
A 		23
C 15.2
(0.2)
A 		21.8
(3)
A 		8.7
(-2.6)
A 		18.5
(1.6)
A 		7.8
(-3)
A 		19.8
(2.1)
A 		14
avr 19.4
(2.3) 		20.8
(3) 		8.5
(-3) 		12.6
(-1) 		8.7
(-2.9) 		19
(2.1) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185217_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4120
Sequence Type Exemplar sequence
Representative Public ID C32B5.15 NCBI
Target Description C32B5.15 /REP_DB=WormBase Gene ID /WP=CE08523 /TR=P91114 /GB=AAB37970.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061469(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061469 NCBI Caenorhabditis elegans C32B5.15 (C32B5.15) mRNA, complete cds. 11/11 None
GENEFINDER00000042186 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:970651:972932:-1 11/11 None
C32B5.15 ENSEMBL cdna:known chromosome:CEL140:II:970651:972956:-1 gene:C32B5.15 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:970649-972955(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C32B5.15
Entrez Gene 183118 Entrez gene
SwissProt P91114 EMBL-EBI
WormBase CE08523 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_493870 C32B5.15 [Caenorhabditis elegans] gb|AAB37970.1| Hypothetical protein C32B5.15 [Caenorhabditis elegans] 1.0E-172
blast NP_493870 C32B5.15 [Caenorhabditis elegans] gb|AAB37970.1| Hypothetical protein C32B5.15 [Caenorhabditis elegans] 1.0E-167
blast AAF39767 Hypothetical protein C39B5.2 [Caenorhabditis elegans] ref|NP_497518.1| C39B5.2 [Caenorhabditis elegans] 8.0E-21
blast AAF39767 Hypothetical protein C39B5.2 [Caenorhabditis elegans] ref|NP_497518.1| C39B5.2 [Caenorhabditis elegans] 9.0E-21
Protein Domains
Database ID Description E-Value
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 0.0014
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 0.0014
Sequence
Target Sequence 
>C. ELEGANS:185217_AT
aacaatggtttactgcgcaactccgtgccatgaagccagcagtataatcaaattttattt
caatggatcaaccttaaaagtagttcacgaaatgcttcgaaacacgattttgaacctcca
ccaaacacatctcaaatcaaatataatatttttttggaagcaaggaatgagtcaacaaaa
gattcaccatttctcaaatattcgaaatgtcaaccacattattctaagtgaaatggttaa
gctagatacaatcccaatcacctttctagatcaaatcttttcgacatggccgaacttagg
cagtttgtcgttgtatccacagatagatggtgaacttaaagatgaatgtgctcccggcta
ccaattgaacgtggaaaacatttatcaagaagttaatgcaaaaccatgggatcctgccac
acgacccatagattattg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACAATGGTTTACTGCGCAACTCCG 335 135 342 Antisense
CTCCGTGCCATGAAGCCAGCAGTAT 395 233 362 Antisense
CTCCACCAAACACATCTCAAATCAA 484 235 457 Antisense
AGCTAGATACAATCCCAATCACCTT 410 79 581 Antisense
AATCCCAATCACCTTTCTAGATCAA 603 167 591 Antisense
AGATCAAATCTTTTCGACATGGCCG 169 67 609 Antisense
GACATGGCCGAACTTAGGCAGTTTG 365 369 624 Antisense
GCAGTTTGTCGTTGTATCCACAGAT 161 313 641 Antisense
TAAAGATGAATGTGCTCCCGGCTAC 606 639 678 Antisense
CTCCCGGCTACCAATTGAACGTGGA 277 231 692 Antisense
CTGCCACACGACCCATAGATTATTG 354 237 755 Antisense
Sequence Source Affymetrix Proprietary Database