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[185208_s_at]

Description Y75B8A.16 /REP_DB=WormBase Gene ID /WP=CE24667 /TR=Q9U1S3 /GB=CAA22103.2 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 99      4grp_highest_8_8 > 19      4grp_high_8_8 > 63      6grp_total > 99      4grp_total > 48      6grp_total_sub > Increase      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 125
1 2 3 4 range
A 106.9
(-3)
P 		166.3
(0.6)
P 		204.9
(3)
P 		148.4
(-0.5)
P 		98
B 164.1
(-0.3)
P 		163.9
(-0.3)
P 		221
(3)
P 		116.2
(-3)
P 		105
C 135.8
(-2.3)
P 		122.9
(-3)
P 		231.4
(3)
P 		166.6
(-0.6)
P 		109
avr 135.6
(-3) 		151
(-1.9) 		219.1
(3) 		143.7
(-2.4) 		84

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 252
1 2 3 4 5 6 range
A 26
(-3)
A 		97
(-0.8)
P 		76
(-1.4)
P 		168.2
(1.4)
P 		199.4
(2.4)
P 		217.9
(3)
P 		192
B 122.1
(-2.5)
P 		142.4
(-1.8)
P 		109.4
(-3)
P 		168.9
(-0.9)
P 		277.8
(3)
P 		219.7
(0.9)
P 		168
C 120.4
(-1.3)
P 		144.8
(-0.3)
P 		79.8
(-2.8)
P 		75.3
(-3)
A 		215.4
(2.4)
P 		230.6
(3)
P 		155
avr 89.5
(-3) 		128.1
(-1.3) 		88.4
(-3) 		137.5
(-0.9) 		230.9
(3) 		222.7
(2.7) 		143
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 59
1 2 3 4 range
A 38.9
(0.2)
P 		36.1
(-0.3)
P 		53.6
(3)
P 		21.7
(-3)
P 		32
B 52.3
(3)
P 		40.5
(0.4)
P 		51.2
(2.8)
P 		24.8
(-3)
P 		28
C 45
(-1.1)
P 		35.3
(-2.2)
P 		80.6
(3)
P 		28.1
(-3)
P 		53
avr 45.4
(0.3) 		37.3
(-1) 		61.8
(3) 		24.9
(-3) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 108
1 2 3 4 5 6 range
A 4.8
(-3)
A 		34.5
(0.2)
P 		16.5
(-1.7)
P 		42.7
(1.1)
P 		60.2
(3)
P 		53.1
(2.2)
P 		55
B 42.5
(-1.9)
P 		47.4
(-1.6)
P 		26.6
(-3)
P 		44.7
(-1.7)
P 		112.9
(3)
P 		81.9
(0.8)
P 		86
C 44.6
(-0.1)
P 		44.2
(-0.2)
P 		20.3
(-2.5)
P 		15.1
(-3)
A 		69.7
(2.3)
P 		77.2
(3)
P 		62
avr 30.6
(-2) 		42
(-0.9) 		21.1
(-3) 		34.2
(-1.7) 		80.9
(3) 		70.7
(2) 		60
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 185208_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7981
Sequence Type Exemplar sequence
Representative Public ID Y75B8A.16 NCBI
Target Description Y75B8A.16 /REP_DB=WormBase Gene ID /WP=CE24667 /TR=Q9U1S3 /GB=CAA22103.2 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067187(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067187 NCBI Caenorhabditis elegans Y75B8A.16 (Y75B8A.16) mRNA, complete cds. 11/11 None
SNAP00000020082 ENSEMBL cdna:SNAP chromosome:CEL140:III:12218935:12223709:1 11/11 None
GENEFINDER00000020169 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:12218935:12223709:1 11/11 None
Y75B8A.16 ENSEMBL cdna:known chromosome:CEL140:III:12218898:12223784:1 gene:Y75B8A.16 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:12218930-12223705(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y75B8A.16
Entrez Gene 176650 Entrez gene
SwissProt Q9U1S3 EMBL-EBI
WormBase CE24667 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:262869_S_AT expressed protein at
CANINE_2:CFAAFFX.17353.1.S1_S_AT similar to G protein-coupled receptor 89 cfa
DROSOPHILA_2:1627473_AT dm
DROSGENOME1:147233_AT dm
CHICKEN:GGA.20065.1.S1_AT G protein-coupled receptor 89 gga
CHICKEN:GGAAFFX.21143.1.S1_S_AT G protein-coupled receptor 89 gga
HG-U133_PLUS_2:220642_X_AT G protein-coupled receptor 89 hs
HG-U133A:220642_X_AT G protein-coupled receptor 89 hs
HG-U133A_2:220642_X_AT G protein-coupled receptor 89 hs
HG-U133B:223531_X_AT G protein-coupled receptor 89 hs
HG-U133_PLUS_2:223531_X_AT G protein-coupled receptor 89 hs
HU35KSUBA:RC_AA232535_S_AT G protein-coupled receptor 89 hs
HU35KSUBA:W73805_AT G protein-coupled receptor 89 hs
U133_X3P:HS.16085.2.A1_3P_A_AT G protein-coupled receptor 89 hs
HG-U133A_2:222140_S_AT G protein-coupled receptor 89 hs
HG-FOCUS:222140_S_AT G protein-coupled receptor 89 hs
HG-U133_PLUS_2:222140_S_AT G protein-coupled receptor 89 hs
HG-U133A:222140_S_AT G protein-coupled receptor 89 hs
HG-U133_PLUS_2:225463_X_AT G protein-coupled receptor 89 hs
HG-U133B:225463_X_AT G protein-coupled receptor 89 hs
HG-U95B:44714_AT G protein-coupled receptor 89 hs
MOE430A:1424696_AT G protein-coupled receptor 89 mm
MOUSE430A_2:1424696_AT G protein-coupled receptor 89 mm
MOUSE430_2:1424696_AT G protein-coupled receptor 89 mm
MG-U74BV2:108985_AT G protein-coupled receptor 89 mm
MU19KSUBA:TC19144_AT G protein-coupled receptor 89 (Gpr89), mRNA mm
RG-U34C:RC_AI070732_AT similar to RIKEN cDNA 4933412D19 (predicted) rn
RAE230A:1372313_AT similar to RIKEN cDNA 4933412D19 (predicted) rn
RAT230_2:1372313_AT similar to RIKEN cDNA 4933412D19 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast CAA22103 Hypothetical protein Y75B8A.16 [Caenorhabditis elegans] ref|NP_499588.1| Y75B8A.16 [Caenorhabditis elegans] 0.0
blast CAE75120 Hypothetical protein CBG23048 [Caenorhabditis briggsae] 0.0
blast CAA94117 Hypothetical protein C11H1.2 [Caenorhabditis elegans] ref|NP_510384.2| C11H1.2 [Caenorhabditis elegans] 1.0E-180
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499588.1 9 13-30,43-65,80-102,114-131,151-173,297-319,347-369,389-411,436-455
Y75B8A.16 9 13-30,43-65,80-102,114-131,151-173,297-319,347-369,389-411,436-455
SNAP00000020082 9 13-30,43-65,80-102,114-131,151-173,297-319,347-369,389-411,436-455
GENEFINDER00000020169 9 13-30,43-65,80-102,114-131,151-173,297-319,347-369,389-411,436-455
Sequence
Target Sequence 
>C. ELEGANS:185208_S_AT
agaaataattccactcgagaccctcgcccgattccttttcctagatctcgtcgaacttcg
ccaaatgttgaatcgtgtggagttcagcaagacattcatgggcatttatttcaacattct
cggacattttttctcaatttattgcatctggaaaatctttatctcgtttattaatatcgt
gttcgatcgagtcggaaaagttgatccggtcacgaaaaccattgaaatcggtgtacattg
gatgggtattccattggatatatcattctggtcacaatatatttcgttttttctcgtcgg
cgttattgcagtcacttctgtcagaggtcttctgatcacaatgacaaagttttttgtgtc
gattagcaatgccacgtcatcattgtcgaatattattgcacttttgatggcacagattat
gggaatgtacttcgtttcgagcgtcctattaatgcgtatgaacgtgcccgaggagtatcg
aacgattctgacgagaattctcggcgatttgaaattcaacttctaccatcgctggttcga
cgtgattttcctaatatccgccgtctcttctattgttttcttgac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAAATAATTCCACTCGAGACCCTC 537 71 786 Antisense
TTCCTAGATCTCGTCGAACTTCGCC 176 699 823 Antisense
TGGGCATTTATTTCAACATTCTCGG 181 555 884 Antisense
ACATTCTCGGACATTTTTTCTCAAT 334 105 899 Antisense
TTCTCGTCGGCGTTATTGCAGTCAC 63 691 1076 Antisense
GCAGTCACTTCTGTCAGAGGTCTTC 109 313 1093 Antisense
GTCGATTAGCAATGCCACGTCATCA 399 473 1143 Antisense
GAATGTACTTCGTTTCGAGCGTCCT 12 331 1208 Antisense
AATTCAACTTCTACCATCGCTGGTT 32 177 1298 Antisense
ACGTGATTTTCCTAATATCCGCCGT 260 95 1325 Antisense
CCGTCTCTTCTATTGTTTTCTTGAC 338 265 1346 Antisense
Sequence Source Affymetrix Proprietary Database