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[185201_s_at]

Description Y116A8C.4 /REP_DB=WormBase Gene ID /WP=CE23315 /TR=Q9U2T1 /GB=CAB55146.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 68      4grp_total > 34      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 4
(-3)
A 		7.8
(-1.3)
A 		11.7
(0.4)
A 		17.5
(3)
A 		14
B 6.5
(-3)
A 		15.5
(3)
A 		7.7
(-2.2)
A 		6.8
(-2.8)
A 		9
C 11.2
(-0.4)
A 		18.6
(3)
A 		5.5
(-3)
A 		15.7
(1.7)
A 		13
avr 7.2
(-3) 		14
(3) 		8.3
(-2) 		13.3
(2.4) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 99
1 2 3 4 5 6 range
A 39
(0.8)
A 		5.1
(-3)
A 		6.4
(-2.9)
A 		12
(-2.2)
A 		9.8
(-2.5)
A 		58
(3)
A 		53
B 6.6
(-2.9)
A 		4.6
(-3)
A 		12.4
(-2.5)
A 		6.7
(-2.9)
A 		11.8
(-2.6)
A 		102.7
(3)
A 		98
C 11.4
(-2.4)
A 		7.4
(-2.7)
A 		4.2
(-3)
A 		10.4
(-2.5)
A 		13.6
(-2.2)
A 		76.3
(3)
A 		72
avr 19
(-1.9) 		5.7
(-3) 		7.7
(-2.8) 		9.7
(-2.7) 		11.7
(-2.5) 		79
(3) 		73
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4
1 2 3 4 range
A 1.4
(-3)
A 		1.7
(-1.9)
A 		3.1
(3)
A 		2.6
(1.2)
A 		2
B 2.1
(-1.4)
A 		3.8
(3)
A 		1.8
(-2.2)
A 		1.5
(-3)
A 		2
C 3.7
(0.2)
A 		5.3
(3)
A 		1.9
(-3)
A 		2.7
(-1.6)
A 		3
avr 2.4
(-2.5) 		3.6
(3) 		2.3
(-3) 		2.3
(-3) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 37
1 2 3 4 5 6 range
A 7.2
(-0.3)
A 		1.8
(-2.8)
A 		1.4
(-3)
A 		3
(-2.2)
A 		3
(-2.2)
A 		14.1
(3)
A 		13
B 2.3
(-2.9)
A 		1.5
(-3)
A 		3
(-2.8)
A 		1.8
(-3)
A 		4.8
(-2.5)
A 		38.3
(3)
A 		37
C 4.2
(-2.2)
A 		2.3
(-2.7)
A 		1.1
(-3)
A 		2.1
(-2.8)
A 		4.4
(-2.2)
A 		25.5
(3)
A 		24
avr 4.6
(-2.3) 		1.9
(-3) 		1.8
(-3) 		2.3
(-2.9) 		4.1
(-2.4) 		26
(3) 		24
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 185201_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8511
Sequence Type Exemplar sequence
Representative Public ID Y116A8C.4 NCBI
Target Description Y116A8C.4 /REP_DB=WormBase Gene ID /WP=CE23315 /TR=Q9U2T1 /GB=CAB55146.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070603(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070603 NCBI Caenorhabditis elegans Y116A8C.4 (Y116A8C.4) mRNA, complete cds. 11/11 None
SNAP00000004073 ENSEMBL cdna:SNAP chromosome:CEL140:IV:16920470:16924030:1 11/11 None
GENEFINDER00000004196 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:16916076:16924224:1 11/11 None
Y116A8C.4.1 ENSEMBL cdna:known chromosome:CEL140:IV:16920342:16924224:1 gene:Y116A8C.4 11/11 None
Y116A8C.4.2 ENSEMBL cdna:known chromosome:CEL140:IV:16920465:16924224:1 gene:Y116A8C.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:16920475-16924230(+) 98.84 100.0
Public Domain and Genome References
Gene Title
Ensembl Y116A8C.4
Entrez Gene 178472 Entrez gene
SwissProt Q9U2T1 EMBL-EBI
WormBase CE23315 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4245 neprilysin activity inferred from electronic annotation QuickGO AmiGO
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB55146 Hypothetical protein Y116A8C.4 [Caenorhabditis elegans] ref|NP_503004.1| Y116A8C.4 [Caenorhabditis elegans] 0.0
blast CAB55147 Hypothetical protein Y116A8C.5 [Caenorhabditis elegans] ref|NP_503005.1| Y116A8C.5 [Caenorhabditis elegans] 1.0E-154
blast CAB55147 Hypothetical protein Y116A8C.5 [Caenorhabditis elegans] ref|NP_503005.1| Y116A8C.5 [Caenorhabditis elegans] 1.0E-153
blast AAB66080 Hypothetical protein F18A12.1 [Caenorhabditis elegans] ref|NP_494537.1| F18A12.1 [Caenorhabditis elegans] 1.0E-153
Protein Domains
Database ID Description E-Value
Pfam IPR008753 EMBL-EBI Peptidase M13 6.6E-6
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 1.1E-58
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 4.0E-59
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 5.9E-10
Pfam IPR008753 EMBL-EBI Peptidase M13 6.6E-6
Pfam IPR000718 EMBL-EBI Peptidase M13, neprilysin 1.1E-58
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503004.1 1 13-35
Y116A8C.4.1 1 13-35
Y116A8C.4.2 1 13-35
SNAP00000004073 1 13-35
GENEFINDER00000004196 3 5-24,309-331,338-360
Sequence
Target Sequence 
>C. ELEGANS:185201_S_AT
cgacctaccccgattatgtgaacattgccatgtctgggaacataattggtcatgaaatgg
gtcacgcatttggtccacacgcaattttgagaactgtcagaggacgggaagtgtggatga
aaccggaagaacttgcagaatacgagagcagggcgcagtgcctggcaaatcagtacagtg
aatatgatgatccagattttggacgagtttttgacggcggcaaagtgatcgatgagcttg
tggcggataatatcggacgggaggtttcttggaaaatgtttaaaagtcttgacttgacaa
atgcgacaaggattattggatttgaagactatgacattgaccaactgtatttccgtgttg
gagccctgacacactgcacccctcatgcgatgcgtgatttgaaagggtctatcgaaagtt
ctcatcctcggaaagcattccgtgtgaatggagtctatgcgaatcttccagagtttgcca
gagcattcaactgcccgacgggatctccaatga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGACCTACCCCGATTATGTGAACAT 499 249 1709 Antisense
ACGCATTTGGTCCACACGCAATTTT 298 91 1772 Antisense
AGGGCGCAGTGCCTGGCAAATCAGT 117 59 1858 Antisense
GTGATCGATGAGCTTGTGGCGGATA 38 483 1933 Antisense
GACATTGACCAACTGTATTTCCGTG 445 369 2041 Antisense
CCCCTCATGCGATGCGTGATTTGAA 329 261 2087 Antisense
GGGTCTATCGAAAGTTCTCATCCTC 35 497 2113 Antisense
AGTTCTCATCCTCGGAAAGCATTCC 150 65 2125 Antisense
GCGAATCTTCCAGAGTTTGCCAGAG 398 287 2167 Antisense
TTTGCCAGAGCATTCAACTGCCCGA 299 669 2182 Antisense
AACTGCCCGACGGGATCTCCAATGA 400 141 2197 Antisense
Sequence Source Affymetrix Proprietary Database