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[185197_at]

Description Y53G8B.4 /REP_DB=WormBase Gene ID /WP=CE25430 /TR=Q9N4S1 /GB=AAF60757.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 49      4grp_total > 12      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 240
1 2 3 4 range
A 214.3
(-0.4)
A 		161.1
(-1.7)
A 		108.8
(-3)
A 		348.3
(3)
A 		240
B 194.2
(-0.7)
A 		117.7
(-3)
A 		141.8
(-2.3)
A 		319.7
(3)
P 		202
C 219.3
(0.3)
A 		215.6
(0.1)
A 		133.6
(-3)
A 		291.2
(3)
M 		158
avr 209.3
(-0.5) 		164.8
(-1.9) 		128.1
(-3) 		319.7
(3) 		192

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 373
1 2 3 4 5 6 range
A 116.1
(-1.9)
A 		205.1
(0.8)
A 		77.8
(-3)
A 		141.8
(-1.1)
A 		121.9
(-1.7)
A 		278.5
(3)
P 		201
B 85.2
(0)
A 		45.1
(-3)
A 		111.8
(2)
A 		75.2
(-0.7)
A 		122.5
(2.8)
A 		125.1
(3)
A 		80
C 387.9
(3)
P 		34.5
(-2.7)
A 		171.2
(-0.5)
A 		15.1
(-3)
A 		230.1
(0.5)
P 		136.3
(-1)
A 		373
avr 196.4
(3) 		94.9
(-2.1) 		120.3
(-0.8) 		77.4
(-3) 		158.2
(1.1) 		180
(2.2) 		119
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 50
1 2 3 4 range
A 78
(3)
A 		35
(-2.2)
A 		28.4
(-3)
A 		51
(-0.3)
A 		50
B 61.9
(2)
A 		29.1
(-3)
A 		32.9
(-2.4)
A 		68.1
(3)
P 		39
C 72.6
(3)
A 		62
(0.6)
A 		46.6
(-3)
A 		49.1
(-2.4)
M 		26
avr 70.8
(3) 		42
(-2) 		36
(-3) 		56.1
(0.5) 		35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 141
1 2 3 4 5 6 range
A 21.4
(-2.5)
A 		72.9
(3)
A 		16.9
(-3)
A 		36
(-1)
A 		36.8
(-0.9)
A 		67.8
(2.5)
P 		56
B 29.7
(-0.5)
A 		15
(-3)
A 		27.2
(-0.9)
A 		19.9
(-2.2)
A 		49.8
(3)
A 		46.6
(2.4)
A 		35
C 143.8
(3)
P 		10.5
(-2.7)
A 		43.6
(-1.3)
A 		3
(-3)
A 		74.4
(0)
P 		45.6
(-1.2)
A 		141
avr 65
(3) 		32.8
(-1.3) 		29.2
(-1.7) 		19.6
(-3) 		53.7
(1.5) 		53.3
(1.5) 		45
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185197_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7383
Sequence Type Exemplar sequence
Representative Public ID Y53G8B.4 NCBI
Target Description Y53G8B.4 /REP_DB=WormBase Gene ID /WP=CE25430 /TR=Q9N4S1 /GB=AAF60757.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065260(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065260 NCBI Caenorhabditis elegans Y53G8B.4 (Y53G8B.4) mRNA, complete cds. 11/11 None
GENEFINDER00000030870 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:3112842:3116995:-1 10/11 None
Y53G8B.4 ENSEMBL cdna:known chromosome:CEL140:III:3112419:3127107:-1 gene:Y53G8B.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3113731-3127086(-) 97.9 97.9
Public Domain and Genome References
Gene Title
Ensembl Y53G8B.4
Entrez Gene 175418 Entrez gene
SwissProt Q9N4S1 EMBL-EBI
WormBase CE33737 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:261164_AT permease-related at
CANINE:1597959_AT similar to non imprinted in Prader-Willi/Angelman syndrome 2 isoform a cfa
CANINE:1588649_AT similar to non imprinted in Prader-Willi/Angelman syndrome 2 isoform a cfa
CANINE:1586982_AT similar to non imprinted in Prader-Willi/Angelman syndrome 2 isoform a cfa
CANINE_2:CFA.15790.1.A1_AT similar to non imprinted in Prader-Willi/Angelman syndrome 2 isoform a cfa
CANINE_2:CFAAFFX.15298.1.S1_S_AT similar to non imprinted in Prader-Willi/Angelman syndrome 2 isoform a cfa
DROSOPHILA_2:1627925_AT dm
DROSGENOME1:153299_AT dm
CHICKEN:GGA.1702.1.S1_AT non imprinted in Prader-Willi/Angelman syndrome 2 gga
HG-U133A:212129_AT non imprinted in Prader-Willi/Angelman syndrome 2 hs
HG-U133A_2:212129_AT non imprinted in Prader-Willi/Angelman syndrome 2 hs
HG-U133_PLUS_2:212129_AT non imprinted in Prader-Willi/Angelman syndrome 2 hs
HUGENEFL:U90904_AT non imprinted in Prader-Willi/Angelman syndrome 2 hs
U133_X3P:HS.83724.0.S1_3P_AT non imprinted in Prader-Willi/Angelman syndrome 2 hs
HG-U95AV2:38452_AT non imprinted in Prader-Willi/Angelman syndrome 2 hs
HG-U95D:73797_AT Non imprinted in Prader-Willi/Angelman syndrome 2 hs
MOUSE430_2:1421968_A_AT non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) mm
MOE430A:1421968_A_AT non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) mm
MOUSE430A_2:1421968_A_AT non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) mm
MOE430B:1459866_X_AT non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) mm
MOUSE430_2:1459866_X_AT non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) mm
MG-U74BV2:107784_AT non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) mm
MG-U74BV2:105975_AT non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) mm
MG-U74BV2:163969_AT non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) mm
MOE430A:1426717_AT non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) mm
MOUSE430A_2:1426717_AT non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) mm
MOUSE430_2:1426717_AT non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human) mm
MG-U74CV2:166488_R_AT Non imprinted in Prader-Willi/Angelman syndrome 2 homolog (human), mRNA (cDNA clone MGC:46749 IMAGE:4482150) mm
RG-U34C:RC_AI071109_AT similar to hypothetical protein MGC5466 (predicted) rn
RAE230B:1377737_AT Similar to hypothetical protein MGC5466 (predicted) rn
RAT230_2:1377737_AT Similar to hypothetical protein MGC5466 (predicted) rn
RG-U34C:RC_AI232332_AT Similar to hypothetical protein MGC5466 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAF60757 Hypothetical protein Y53G8B.4 [Caenorhabditis elegans] ref|NP_497661.2| Y53G8B.4 [Caenorhabditis elegans] 0.0
blast CAE72971 Hypothetical protein CBG20308 [Caenorhabditis briggsae] 1.0E-150
blast AAF60757 Hypothetical protein Y53G8B.4 [Caenorhabditis elegans] ref|NP_497661.2| Y53G8B.4 [Caenorhabditis elegans] 1.0E-122
blast CAE72971 Hypothetical protein CBG20308 [Caenorhabditis briggsae] 5.0E-95
blast XP_625083 PREDICTED: similar to ENSANGP00000015795 [Apis mellifera] 5.0E-84
blast XP_625083 PREDICTED: similar to ENSANGP00000015795 [Apis mellifera] 3.0E-56
Protein Domains
Database ID Description E-Value
Pfam IPR008521 EMBL-EBI Protein of unknown function DUF803 1.0E-126
Pfam IPR008521 EMBL-EBI Protein of unknown function DUF803 5.8E-37
Pfam IPR008521 EMBL-EBI Protein of unknown function DUF803 8.0E-17
Pfam IPR008521 EMBL-EBI Protein of unknown function DUF803 1.1E-46
Pfam IPR008521 EMBL-EBI Protein of unknown function DUF803 3.8E-54
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497661.2 9 54-76,104-126,131-148,155-176,196-218,225-247,251-273,280-302,317-336
Y53G8B.4 9 54-76,104-126,131-148,155-176,196-218,225-247,251-273,280-302,317-336
GENEFINDER00000030870 6 35-57,240-262,282-304,325-347,357-379,391-409
Sequence
Target Sequence 
>C. ELEGANS:185197_AT
gtgacagcaattctatcatcccgaatgctaaacgaacgtctaaatcttctcggctcaatc
ggttgtgctctttgcctcctcggctccaccgtaatcgtaattcattcgccgaaagaagag
gaagtcggttcgatggcagacttggcgctgaaaatgaaagacgcgggatttttgatctac
gtgatactcattattcttgcgacgggcttcatcgttgtctatgtggctccgcgttacggt
cattcgaatattttagtgtacatttctgtgtgctcactcattggatcattatcagttctt
tcggaaacccttgccggcaaccaacaattcaccaactggctcacctatttttggctggcg
tcagtggcgatgtgtgtctcggtacagttgatttatctcaacaaagctctggatatcttt
aatacttcaatggtgacaccgatttattacgtcttcttcac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGACAGCAATTCTATCATCCCGAA 250 483 436 Antisense
ACGAACGTCTAAATCTTCTCGGCTC 123 93 467 Antisense
TCGGCTCCACCGTAATCGTAATTCA 380 607 515 Antisense
TCGGTTCGATGGCAGACTTGGCGCT 139 607 560 Antisense
GATACTCATTATTCTTGCGACGGGC 21 425 618 Antisense
GGCTTCATCGTTGTCTATGTGGCTC 263 537 640 Antisense
GCTCCGCGTTACGGTCATTCGAATA 456 299 661 Antisense
ATTCACCAACTGGCTCACCTATTTT 102 13 762 Antisense
TCACCTATTTTTGGCTGGCGTCAGT 48 627 776 Antisense
GTCAGTGGCGATGTGTGTCTCGGTA 435 465 795 Antisense
CACCGATTTATTACGTCTTCTTCAC 415 201 872 Antisense
Sequence Source Affymetrix Proprietary Database