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[185193_at]

Description Y50E8A.12 /REP_DB=WormBase Gene ID /WP=CE22319 /TR=Q9NAE6 /GB=CAB55060.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 95      4grp_total > 28      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 68
1 2 3 4 range
A 29
(-1.9)
A 		39.8
(-0.6)
M 		20
(-3)
A 		69.8
(3)
P 		50
B 38.9
(3)
A 		25.5
(0.8)
A 		1.7
(-3)
A 		30.7
(1.7)
A 		37
C 30.4
(-0.5)
A 		50
(2.5)
A 		14.1
(-3)
A 		53.2
(3)
P 		39
avr 32.8
(0.2) 		38.4
(1) 		11.9
(-3) 		51.2
(3) 		39

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 125
1 2 3 4 5 6 range
A 45.6
(-2.4)
A 		44.9
(-2.4)
A 		36.6
(-3)
M 		67.2
(-0.9)
P 		122
(3)
P 		72.4
(-0.5)
P 		85
B 21
(-2.1)
A 		8
(-2.8)
A 		3.6
(-3)
A 		64.6
(0.3)
P 		108.5
(2.6)
P 		115
(3)
P 		111
C 32.1
(-2.8)
A 		74.2
(-0.3)
P 		29.1
(-3)
A 		43.1
(-2.2)
A 		93.3
(0.9)
P 		128.5
(3)
P 		99
avr 32.9
(-2.3) 		42.4
(-1.6) 		23.1
(-3) 		58.3
(-0.5) 		107.9
(3) 		105.3
(2.8) 		85
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 10.6
(3)
A 		8.6
(0.8)
M 		5.2
(-3)
A 		10.2
(2.6)
P 		5
B 12.4
(3)
A 		6.3
(-0.1)
A 		0.4
(-3)
A 		6.5
(0)
A 		12
C 10.1
(0.3)
A 		14.4
(3)
A 		4.9
(-3)
A 		9
(-0.4)
P 		10
avr 11
(3) 		9.8
(2) 		3.5
(-3) 		8.6
(1.1) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 43
1 2 3 4 5 6 range
A 8.4
(-2.9)
A 		16
(-1.3)
A 		8
(-3)
M 		17
(-1.1)
P 		36.8
(3)
P 		17.6
(-1)
P 		29
B 7.3
(-2.1)
A 		2.7
(-2.8)
A 		0.9
(-3)
A 		17.1
(-0.8)
P 		44.1
(3)
P 		42.9
(2.8)
P 		43
C 11.9
(-2.2)
A 		22.6
(-0.4)
P 		7.4
(-3)
A 		8.7
(-2.8)
A 		30.2
(0.8)
P 		43
(3)
P 		36
avr 9.2
(-2.3) 		13.8
(-1.4) 		5.4
(-3) 		14.3
(-1.3) 		37
(3) 		34.5
(2.5) 		32
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185193_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13661
Sequence Type Exemplar sequence
Representative Public ID Y50E8A.12 NCBI
Target Description Y50E8A.12 /REP_DB=WormBase Gene ID /WP=CE22319 /TR=Q9NAE6 /GB=CAB55060.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074248(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074248 NCBI Caenorhabditis elegans Y50E8A.12 (Y50E8A.12) mRNA, complete cds. 11/11 None
SNAP00000008995 ENSEMBL cdna:SNAP chromosome:CEL140:V:14785301:14786590:-1 11/11 None
GENEFINDER00000009976 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14784893:14786590:-1 11/11 None
Y50E8A.12 ENSEMBL cdna:known chromosome:CEL140:V:14785301:14786590:-1 gene:Y50E8A.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14785300-14786590(-) 99.09 100.0
Public Domain and Genome References
Gene Title
Ensembl Y50E8A.12
Entrez Gene 190112 Entrez gene
SwissProt Q9NAE6 EMBL-EBI
WormBase CE22319 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB55060 Hypothetical protein Y50E8A.12 [Caenorhabditis elegans] ref|NP_506649.1| Y50E8A.12 [Caenorhabditis elegans] 1.0E-101
blast CAE66360 Hypothetical protein CBG11617 [Caenorhabditis briggsae] 1.0E-28
blast CAE66360 Hypothetical protein CBG11617 [Caenorhabditis briggsae] 2.0E-28
Sequence
Target Sequence 
>C. ELEGANS:185193_AT
ctctccgaagcttcttctgtgcaatcaccgactgtttcgattcgacgtcgaacttgtgtc
gtttgtggatcctcgacgcttaacagctacgtcacttctttcacaagagttccagacaaa
caagaacaatgggtgactgtgttggcgaatggagacgcaggattcgaagatcagttgaag
gcttcattggcgactggcagaaaatacatctgctacgatcatttcgacaggcagtatttt
gcacaaaggaagactgacgacggatttgaattgatccggaacaggaacccgatggcattt
aaaaacgctactttcatccgacacgtggatctcagaagcacttcatcatttgtgccagac
gactcatcagaggaaccgatccctgctaaacgtctgaaaataatgagtgatttgaagggt
agaggtggagaattggatccgaattcgatgactttcctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTCTCCGAAGCTTCTTCTGTGCAAT 689 229 16 Antisense
TCACCGACTGTTTCGATTCGACGTC 45 627 40 Antisense
GAACTTGTGTCGTTTGTGGATCCTC 187 351 65 Antisense
GATCCTCGACGCTTAACAGCTACGT 455 415 83 Antisense
TTAACAGCTACGTCACTTCTTTCAC 388 683 95 Antisense
TTGAAGGCTTCATTGGCGACTGGCA 182 703 190 Antisense
TAAAAACGCTACTTTCATCCGACAC 547 639 315 Antisense
GACACGTGGATCTCAGAAGCACTTC 256 367 335 Antisense
TCATTTGTGCCAGACGACTCATCAG 379 619 361 Antisense
TCATCAGAGGAACCGATCCCTGCTA 302 621 379 Antisense
GGATCCGAATTCGATGACTTTCCTG 462 513 450 Antisense
Sequence Source Affymetrix Proprietary Database