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[185180_at]

Description F14D2.11 /REP_DB=WormBase Gene ID /WP=CE16986 /TR=O44803 /GB=AAB94965.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 96      4grp_total > 08      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 245
1 2 3 4 range
A 5.8
(-3)
A 		5.9
(-3)
A 		82.2
(0.3)
A 		143.3
(3)
P 		138
B 8.6
(-3)
A 		9.7
(-3)
A 		56.8
(-1.5)
A 		198.2
(3)
P 		190
C 12.8
(-3)
A 		37.4
(-2.4)
A 		25.8
(-2.7)
A 		250.5
(3)
P 		238
avr 9.1
(-3) 		17.7
(-2.7) 		54.9
(-1.5) 		197.3
(3) 		188

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 215
1 2 3 4 5 6 range
A 10.4
(-2.8)
A 		7.1
(-3)
A 		47.8
(-0.6)
A 		48.2
(-0.6)
A 		72.3
(0.8)
P 		110.3
(3)
P 		103
B 8.7
(-2.9)
A 		6.6
(-3)
A 		63.9
(-0.5)
A 		78
(0.1)
A 		110.8
(1.5)
P 		144.6
(3)
P 		138
C 8.1
(-3)
A 		23.9
(-2.6)
A 		11.6
(-2.9)
A 		43.4
(-2)
A 		171
(1.6)
P 		222
(3)
P 		214
avr 9.1
(-3) 		12.5
(-2.9) 		41.1
(-1.7) 		56.5
(-1.1) 		118
(1.4) 		159
(3) 		150
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 41
1 2 3 4 range
A 2.1
(-2.8)
A 		1.3
(-3)
A 		21.5
(3)
A 		21
(2.9)
P 		20
B 2.7
(-3)
A 		2.4
(-3)
A 		13.2
(-1.4)
A 		42.2
(3)
P 		40
C 4.2
(-3)
A 		10.8
(-2)
A 		9
(-2.2)
A 		42.2
(3)
P 		38
avr 3
(-3) 		4.8
(-2.7) 		14.6
(-0.8) 		35.1
(3) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 72
1 2 3 4 5 6 range
A 1.9
(-3)
A 		2.5
(-2.9)
A 		10.4
(-1)
A 		12.2
(-0.5)
A 		21.8
(1.8)
P 		26.9
(3)
P 		25
B 3
(-2.9)
A 		2.2
(-3)
A 		15.5
(-1.5)
A 		20.7
(-0.9)
A 		45
(2)
P 		53.9
(3)
P 		52
C 3
(-3)
A 		7.3
(-2.6)
A 		2.9
(-3)
A 		8.7
(-2.5)
A 		55.3
(1.4)
P 		74.3
(3)
P 		71
avr 2.6
(-3) 		4
(-2.8) 		9.6
(-2.1) 		13.9
(-1.6) 		40.7
(1.7) 		51.7
(3) 		49
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185180_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4545
Sequence Type Exemplar sequence
Representative Public ID F14D2.11 NCBI
Target Description F14D2.11 /REP_DB=WormBase Gene ID /WP=CE16986 /TR=O44803 /GB=AAB94965.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062110(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062110 NCBI Caenorhabditis elegans F14D2.11 (F14D2.11) mRNA, complete cds. 11/11 None
GENEFINDER00000037081 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3341374:3342066:-1 9/11 None
F14D2.11 ENSEMBL cdna:known chromosome:CEL140:II:3341374:3341762:-1 gene:F14D2.11 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000037059 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3341372-3341761(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F14D2.11
Entrez Gene 184461 Entrez gene
SwissProt O44803 EMBL-EBI
WormBase CE16986 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_494511 F14D2.11 [Caenorhabditis elegans] gb|AAB94965.1| Hypothetical protein F14D2.11 [Caenorhabditis elegans] 8.0E-58
blast NP_494511 F14D2.11 [Caenorhabditis elegans] gb|AAB94965.1| Hypothetical protein F14D2.11 [Caenorhabditis elegans] 1.0E-45
Protein Domains
Database ID Description E-Value
Pfam IPR000626 EMBL-EBI Ubiquitin 3.7E-14
Pfam IPR000626 EMBL-EBI Ubiquitin 3.3E-14
Sequence
Target Sequence 
>C. ELEGANS:185180_AT
actatcgctaaatgagatgctacgtaaaagatgtcttcaagtaaagaatacaaagatgaa
aaattcttccatgccaaagttccagtattttgttcgattaaatggaaaaactcgcactct
taacgttaatgcttcggatacagttgagcaaggaaaaatgcaattatgtcataatgctag
gagtacacggatgagctacggaggaaagccgctctccgatcagatcacttttggcgagta
caacatcagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTATCGCTAAATGAGATGCTACGT 461 101 60 Antisense
GATGCTACGTAAAAGATGTCTTCAA 579 413 75 Antisense
AATTCTTCCATGCCAAAGTTCCAGT 321 177 121 Antisense
GCCAAAGTTCCAGTATTTTGTTCGA 371 277 132 Antisense
ATGGAAAAACTCGCACTCTTAACGT 226 53 161 Antisense
AACTCGCACTCTTAACGTTAATGCT 178 141 168 Antisense
CGTTAATGCTTCGGATACAGTTGAG 441 245 183 Antisense
GGAGTACACGGATGAGCTACGGAGG 565 517 239 Antisense
GCTACGGAGGAAAGCCGCTCTCCGA 490 301 254 Antisense
GCTCTCCGATCAGATCACTTTTGGC 669 299 270 Antisense
CACTTTTGGCGAGTACAACATCAGC 664 195 285 Antisense
Sequence Source Affymetrix Proprietary Database