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[185177_at]

Description H10E21.1 /REP_DB=WormBase Gene ID /WP=CE19486 /TR=O76669 /GB=AAC26919.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 97      6grp_high1_10_10 > 78      4grp_highest_8_8 > 14      4grp_high_8_8 > 14      6grp_highest_nonorm > 81      6grp_total > 68      4grp_total > 09      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 425
1 2 3 4 range
A 231.8
(-3)
P 		325.3
(-1.7)
P 		242.9
(-2.8)
P 		654.3
(3)
P 		423
B 229.1
(-3)
P 		261.1
(-2.3)
P 		341.2
(-0.6)
P 		506.4
(3)
P 		277
C 253.1
(-3)
P 		303.5
(-1.9)
P 		291.3
(-2.2)
P 		523.2
(3)
P 		270
avr 238
(-3) 		296.6
(-1.9) 		291.8
(-2) 		561.3
(3) 		323

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 207
1 2 3 4 5 6 range
A 304.6
(2.3)
P 		220.6
(-3)
P 		316.3
(3)
P 		254.4
(-0.9)
P 		260.6
(-0.5)
P 		300.8
(2)
P 		96
B 310.6
(1.1)
P 		227.4
(-3)
P 		228.9
(-2.9)
P 		264
(-1.2)
P 		296.7
(0.4)
P 		350.1
(3)
P 		123
C 235.3
(-1.9)
P 		205.5
(-2.8)
P 		198.8
(-3)
P 		264.3
(-1.1)
P 		252.1
(-1.5)
P 		405.4
(3)
P 		207
avr 283.5
(-0.1) 		217.8
(-3) 		248
(-1.7) 		260.9
(-1.1) 		269.8
(-0.7) 		352.1
(3) 		134
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 44
1 2 3 4 range
A 84.4
(0.9)
P 		70.6
(-1.7)
P 		63.5
(-3)
P 		95.8
(3)
P 		32
B 73
(-1.8)
P 		64.5
(-3)
P 		79.1
(-1)
P 		107.9
(3)
P 		43
C 83.8
(-3)
P 		87.3
(-1.8)
P 		101.5
(3)
P 		88.2
(-1.5)
P 		18
avr 80.4
(-1.4) 		74.1
(-3) 		81.4
(-1.1) 		97.3
(3) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 85
1 2 3 4 5 6 range
A 56.1
(-3)
P 		78.4
(2.9)
P 		68.9
(0.4)
P 		64.5
(-0.8)
P 		78.6
(3)
P 		73.2
(1.6)
P 		23
B 108.1
(1.2)
P 		75.7
(-1.4)
P 		55.6
(-3)
P 		69.9
(-1.9)
P 		120.5
(2.2)
P 		130.6
(3)
P 		75
C 87.2
(-0.4)
P 		62.7
(-2.1)
P 		50.6
(-3)
P 		53.1
(-2.8)
P 		81.6
(-0.8)
P 		135.7
(3)
P 		85
avr 83.8
(-0.2) 		72.3
(-1.5) 		58.4
(-3) 		62.5
(-2.6) 		93.6
(0.9) 		113.2
(3) 		55
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185177_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8165
Sequence Type Exemplar sequence
Representative Public ID H10E21.1 NCBI
Target Description H10E21.1 /REP_DB=WormBase Gene ID /WP=CE19486 /TR=O76669 /GB=AAC26919.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064726
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
SNAP00000020264 ENSEMBL cdna:SNAP chromosome:CEL140:III:15816:17003:1 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_064726 8/11 Cross Hyb Matching Probes None
GENEFINDER00000020268 6/11 Cross Hyb Matching Probes None
GENEFINDER00000020267 4/11 Cross Hyb Matching Probes None
H10E21.1 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:15719-17279(+) 100.0 100.0
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE69166 Hypothetical protein CBG15198 [Caenorhabditis briggsae] 1.0E-41
blast AAC26919 Hypothetical protein H10E21.1 [Caenorhabditis elegans] ref|NP_497127.2| H10E21.1 [Caenorhabditis elegans] 2.0E-16
Sequence
Target Sequence 
>C. ELEGANS:185177_AT
tccgactacgaggacgcggtgtcaagtggcgaggagctctcaaaattgtgtgattcctta
aaatctcatctgggtactgtaaccaaaatgcggaggagtaagaaaagtgtctcgaaactt
ttgtcgaatcaggaacggatggctcagtactatagccgcgagtcgagcagcgagcactcg
agtttggaaaagcttcaacccgatgaaatcaaaaaccggagaattacggggccatcaaag
gggcgacggagcaacagtggaagtgaccacagcagtgactggaaagtactcaatttggcg
gtaaattcgaattttttaaccaatttttacccaaaaatttcaaattttcagttccaagaa
atggctcactacccacttttaatgcagctcaatgagcaacttatggaaatttcaaggcag
ctgaatagcgtcgaaactaccaatgcactacaattaaaggttatcatgcatctgttgaat
cgggtttctaagcgccgaaccacgtggactcagtcgatttgccgctttttccggaatttt
caatttttctttttcgcattctcctggcctttcatagcacggttac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCGACTACGAGGACGCGGTGTCAA 663 591 172 Antisense
GGTGTCAAGTGGCGAGGAGCTCTCA 322 499 189 Antisense
ATTCCTTAAAATCTCATCTGGGTAC 213 7 224 Antisense
GGATGGCTCAGTACTATAGCCGCGA 213 515 308 Antisense
ATAGCCGCGAGTCGAGCAGCGAGCA 306 23 323 Antisense
GGGGCCATCAAAGGGGCGACGGAGC 122 497 399 Antisense
AGAAATGGCTCACTACCCACTTTTA 241 71 528 Antisense
ACTACCCACTTTTAATGCAGCTCAA 452 101 539 Antisense
GCGTCGAAACTACCAATGCACTACA 286 289 599 Antisense
ACGTGGACTCAGTCGATTTGCCGCT 604 95 673 Antisense
TCCTGGCCTTTCATAGCACGGTTAC 208 593 733 Antisense
Sequence Source Affymetrix Proprietary Database