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[185156_at]

Description R09E12.2 /REP_DB=WormBase Gene ID /WP=CE12644 /TR=O16260 /GB=AAG24171.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 39      4grp_total > 0      6grp_cgc > 89      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 55
1 2 3 4 range
A 57.3
(-1.4)
A 		42.4
(-3)
A 		79.5
(1.1)
A 		96.9
(3)
A 		55
B 53.8
(-2.2)
A 		82.8
(3)
A 		49.2
(-3)
A 		74.1
(1.4)
A 		34
C 97.2
(3)
A 		64.5
(-3)
A 		81.7
(0.2)
A 		97.2
(3)
A 		33
avr 69.4
(-1.6) 		63.2
(-3) 		70.1
(-1.4) 		89.4
(3) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 124
1 2 3 4 5 6 range
A 79.7
(0.4)
A 		8.9
(-3)
A 		34.3
(-1.8)
A 		14.8
(-2.7)
A 		132.8
(3)
A 		83.5
(0.6)
A 		124
B 66
(1.1)
A 		86.9
(3)
A 		51.9
(-0.2)
A 		40.5
(-1.2)
A 		20.7
(-3)
A 		43.2
(-1)
A 		66
C 47.9
(-0.6)
A 		45.3
(-0.8)
A 		14.7
(-3)
A 		76.5
(1.5)
A 		97.4
(3)
A 		80.2
(1.8)
A 		83
avr 64.5
(0.7) 		47
(-1.4) 		33.6
(-3) 		43.9
(-1.8) 		83.6
(3) 		69
(1.2) 		50
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 20.9
(3)
A 		9.2
(-3)
A 		20.8
(2.9)
A 		14.2
(-0.4)
A 		12
B 17.2
(0.8)
A 		20.5
(3)
A 		11.4
(-3)
A 		15.8
(-0.1)
A 		9
C 32.2
(3)
A 		18.5
(-2.2)
A 		28.5
(1.6)
A 		16.4
(-3)
A 		16
avr 23.4
(3) 		16.1
(-2.5) 		20.2
(0.6) 		15.5
(-3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 37
1 2 3 4 5 6 range
A 14.7
(-1.1)
A 		3.2
(-3)
A 		7.5
(-2.3)
A 		3.7
(-2.9)
A 		40.1
(3)
A 		20.3
(-0.2)
A 		37
B 23
(1.3)
A 		28.9
(3)
A 		12.6
(-1.8)
A 		10.7
(-2.3)
A 		8.4
(-3)
A 		16.1
(-0.7)
A 		21
C 17.8
(0)
A 		13.8
(-0.8)
A 		3.7
(-3)
A 		15.4
(-0.5)
A 		31.5
(3)
A 		26.8
(2)
A 		28
avr 18.5
(0.4) 		15.3
(-0.6) 		7.9
(-3) 		9.9
(-2.4) 		26.7
(3) 		21.1
(1.2) 		19
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185156_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15142
Sequence Type Exemplar sequence
Representative Public ID R09E12.2 NCBI
Target Description R09E12.2 /REP_DB=WormBase Gene ID /WP=CE12644 /TR=O16260 /GB=AAG24171.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070922(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070922 NCBI Caenorhabditis elegans Serpentine Receptor, class BC (class B-like) family member (srbc-58) (srbc-58) mRNA, complete cds. 11/11 None
R09E12.2 ENSEMBL cdna:known chromosome:CEL140:V:774825:777102:1 gene:R09E12.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:774824-777102(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol srbc-58
Ensembl R09E12.2
Entrez Gene 187746 Entrez gene
SwissProt O16260 EMBL-EBI
WormBase CE12644 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAG24171 Serpentine receptor, class bc (class b-like) protein 58 [Caenorhabditis elegans] ref|NP_503323.1| Serpentine Receptor, class BC (class B-like) family member (srbc-58) [Caenorhabditis elegans] 1.0E-158
blast AAW88392 Serpentine receptor, class bc (class b-like) protein 60 [Caenorhabditis elegans] ref|NP_001024074.1| Serpentine Receptor, class BC (class B-like) family member (srbc-60) [Caenorhabditis elegans] 9.0E-76
blast CAE74133 Hypothetical protein CBG21804 [Caenorhabditis briggsae] 5.0E-75
Protein Domains
Database ID Description E-Value
Pfam IPR002892 EMBL-EBI Protein of unknown function DUF40 4.1E-107
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503323.1 5 4-26,46-68,83-102,123-145,175-197
R09E12.2 5 4-26,46-68,83-102,123-145,175-197
Sequence
Target Sequence 
>C. ELEGANS:185156_AT
ttggcccggcttcaacttgggaactattcgctgctggattgtattttttataacatccga
tagaattttcgcaacatgcgctccgatttcatatcacaatcatcgattcaaaattccgct
cgccgtgattatcatcctcatctccgcgtacacaattctcgagcaatatattttattcgg
atattgcgattttgttctagacgttcccgccgattgtgccattttccggtgtgcaattaa
tatttgctatcacgagtattggaaaactttttcgcagacgatgtatttttgcattggaac
gttgacatttattttattttttcgacttttcatatggaatcattttatactacgttccac
taatacagctatttcaagagccacaaaaatcacgcttctcgactccttcatcatattttc
atttgatctattcccggtttttctcacagcccgattcccggacatcaattctcgaactgt
cgggccgttgagtgctttattga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGGCCCGGCTTCAACTTGGGAACT 516 553 267 Antisense
GGGAACTATTCGCTGCTGGATTGTA 222 495 285 Antisense
TTCGCAACATGCGCTCCGATTTCAT 349 697 334 Antisense
CATCGATTCAAAATTCCGCTCGCCG 578 211 367 Antisense
TCTCCGCGTACACAATTCTCGAGCA 275 611 407 Antisense
TGCGATTTTGTTCTAGACGTTCCCG 554 557 451 Antisense
CCGATTGTGCCATTTTCCGGTGTGC 136 265 476 Antisense
CACAAAAATCACGCTTCTCGACTCC 147 195 648 Antisense
TTTGATCTATTCCCGGTTTTTCTCA 700 665 688 Antisense
GATTCCCGGACATCAATTCTCGAAC 434 433 719 Antisense
GTCGGGCCGTTGAGTGCTTTATTGA 288 473 745 Antisense
Sequence Source Affymetrix Proprietary Database