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[185145_at]

Description Y46D2A.2 /REP_DB=WormBase Gene ID /WP=CE25300 /TR=Q9N4X1 /GB=AAF60610.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 65      4grp_highest_8_8 > 13      4grp_high_8_8 > 31      6grp_total > 55      4grp_total > 24      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 312
1 2 3 4 range
A 181.9
(-3)
P 		315.3
(-0.4)
P 		414.8
(1.5)
P 		493.8
(3)
P 		312
B 199.2
(-3)
P 		292
(-0.6)
P 		387.6
(1.8)
P 		435.7
(3)
P 		237
C 194.6
(-3)
P 		318.7
(0.4)
P 		322.6
(0.5)
P 		412.9
(3)
P 		218
avr 191.9
(-3) 		308.7
(-0.3) 		375
(1.3) 		447.5
(3) 		256

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 130
1 2 3 4 5 6 range
A 107.5
(-1.7)
P 		105.1
(-1.8)
P 		105.1
(-1.8)
P 		135
(-0.1)
P 		85.3
(-3)
P 		188.6
(3)
P 		103
B 109.8
(0.1)
P 		102.5
(-0.3)
P 		156.5
(3)
P 		58.5
(-3)
P 		60.8
(-2.9)
A 		115.5
(0.5)
P 		98
C 118.8
(0)
P 		94.5
(-2.4)
P 		122
(0.4)
P 		112.9
(-0.6)
P 		88.7
(-3)
P 		148
(3)
P 		59
avr 112
(-0.2) 		100.7
(-1.1) 		127.9
(1.1) 		102.1
(-1) 		78.3
(-3) 		150.7
(3) 		72
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 49
1 2 3 4 range
A 66.2
(-3)
P 		68.4
(-2.7)
P 		108.4
(3)
P 		72.3
(-2.1)
P 		42
B 63.5
(-3)
P 		72.1
(-1.2)
P 		89.8
(2.4)
P 		92.8
(3)
P 		29
C 64.4
(-3)
P 		91.6
(0.4)
P 		112.4
(3)
P 		69.6
(-2.4)
P 		48
avr 64.7
(-3) 		77.4
(-1) 		103.5
(3) 		78.2
(-0.9) 		39

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 34
1 2 3 4 5 6 range
A 19.8
(-3)
P 		37.4
(1)
P 		22.9
(-2.3)
P 		34.2
(0.3)
P 		25.7
(-1.6)
P 		45.9
(3)
P 		26
B 38.2
(1.9)
P 		34.1
(1)
P 		38
(1.9)
P 		15.5
(-3)
P 		24.7
(-1)
A 		43.1
(3)
P 		28
C 44
(1.8)
P 		28.8
(-1.6)
P 		31.1
(-1.1)
P 		22.7
(-3)
P 		28.7
(-1.7)
P 		49.5
(3)
P 		27
avr 34
(-0.3) 		33.4
(-0.5) 		30.7
(-1.2) 		24.1
(-3) 		26.4
(-2.4) 		46.2
(3) 		22
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 185145_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5370
Sequence Type Exemplar sequence
Representative Public ID Y46D2A.2 NCBI
Target Description Y46D2A.2 /REP_DB=WormBase Gene ID /WP=CE25300 /TR=Q9N4X1 /GB=AAF60610.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062104(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062104 NCBI Caenorhabditis elegans Y46D2A.2 (Y46D2A.2) mRNA, complete cds. 11/11 None
SNAP00000014491 ENSEMBL cdna:SNAP chromosome:CEL140:II:3320556:3323433:-1 11/11 None
GENEFINDER00000014494 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3320556:3323176:-1 11/11 None
Y46D2A.2 ENSEMBL cdna:known chromosome:CEL140:II:3321415:3323176:-1 gene:Y46D2A.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_062103 6/11 Cross Hyb Matching Probes None
SNAP00000014490 4/11 Cross Hyb Matching Probes None
GENEFINDER00000014496 4/11 Cross Hyb Matching Probes None
SNAP00000020872 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3318296-3319937(+) 97.45 97.45
chrII:3321501-3323175(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y46D2A.2
Entrez Gene 189952 Entrez gene
SwissProt Q9N4X1 EMBL-EBI
WormBase CE38645 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_494505 Y46D2A.2 [Caenorhabditis elegans] gb|AAF60610.3| Hypothetical protein Y46D2A.2 [Caenorhabditis elegans] 1.0E-167
blast NP_494505 Y46D2A.2 [Caenorhabditis elegans] gb|AAF60610.3| Hypothetical protein Y46D2A.2 [Caenorhabditis elegans] 1.0E-164
blast AAB70366 Hypothetical protein F39E9.1 [Caenorhabditis elegans] ref|NP_494504.2| F39E9.1 [Caenorhabditis elegans] 1.0E-155
blast AAB70366 Hypothetical protein F39E9.1 [Caenorhabditis elegans] ref|NP_494504.2| F39E9.1 [Caenorhabditis elegans] 1.0E-153
blast NP_494505 Y46D2A.2 [Caenorhabditis elegans] gb|AAF60610.3| Hypothetical protein Y46D2A.2 [Caenorhabditis elegans] 1.0E-135
blast AAB70366 Hypothetical protein F39E9.1 [Caenorhabditis elegans] ref|NP_494504.2| F39E9.1 [Caenorhabditis elegans] 1.0E-126
blast NP_494505 Y46D2A.2 [Caenorhabditis elegans] gb|AAF60610.3| Hypothetical protein Y46D2A.2 [Caenorhabditis elegans] 1.0E-126
blast AAB70366 Hypothetical protein F39E9.1 [Caenorhabditis elegans] ref|NP_494504.2| F39E9.1 [Caenorhabditis elegans] 1.0E-122
blast NP_494506 Y46D2A.1 [Caenorhabditis elegans] gb|AAF60609.2| Hypothetical protein Y46D2A.1 [Caenorhabditis elegans] 8.0E-65
blast NP_494506 Y46D2A.1 [Caenorhabditis elegans] gb|AAF60609.2| Hypothetical protein Y46D2A.1 [Caenorhabditis elegans] 3.0E-64
Protein Domains
Database ID Description E-Value
Pfam IPR005071 EMBL-EBI Protein of unknown function DUF274 1.6E-31
Pfam IPR005071 EMBL-EBI Protein of unknown function DUF274 3.6E-48
Pfam IPR005071 EMBL-EBI Protein of unknown function DUF274 1.4E-48
Pfam IPR005071 EMBL-EBI Protein of unknown function DUF274 3.6E-48
Pfam IPR005071 EMBL-EBI Protein of unknown function DUF274 2.6E-34
Sequence
Target Sequence 
>C. ELEGANS:185145_AT
tatcgtcatcgatggcgggctagtaatttctacaagcaattccaacgcggacacttctgg
cctaaccggcttcctgtaccttaccacgaaaaagcaagctgatgatccagcattctccgt
ctatgtcatcaagcaagctcaaacagtgtccacgactcagccaaactccaccgtcgtaat
cttgaataccgaacttgataatgacgcattcttcgatcacattgaaccacttcaaatcgg
actggactactggtacttcacagcttcagagccaatgagcatgacgatggagagcaagta
tgtgagcgatctggtctacaccactacttcagttaacactaccgggcttgttgtcaatga
ctttctcttccaggagcatgttgtcaagttccagttggatctgacacgtgtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATCGTCATCGATGGCGGGCTAGTA 654 647 252 Antisense
AAGCAATTCCAACGCGGACACTTCT 507 149 285 Antisense
AGCTGATGATCCAGCATTCTCCGTC 277 81 348 Antisense
ATTCTCCGTCTATGTCATCAAGCAA 342 11 363 Antisense
ATAATGACGCATTCTTCGATCACAT 221 25 449 Antisense
AATCGGACTGGACTACTGGTACTTC 595 167 486 Antisense
GGTACTTCACAGCTTCAGAGCCAAT 84 505 503 Antisense
ATGTGAGCGATCTGGTCTACACCAC 410 49 551 Antisense
ACACTACCGGGCTTGTTGTCAATGA 321 109 587 Antisense
TTGTCAATGACTTTCTCTTCCAGGA 546 707 602 Antisense
GTTCCAGTTGGATCTGACACGTGTC 405 441 639 Antisense
Sequence Source Affymetrix Proprietary Database